PMID- 36845326
OWN - NLM
STAT- MEDLINE
DCOM- 20230228
LR  - 20231116
IS  - 2046-1402 (Electronic)
IS  - 2046-1402 (Linking)
VI  - 11
DP  - 2022
TI  - Submicron 3-D mass spectrometry imaging reveals an asymmetric molecular 
      distribution on chemotaxing cells.
PG  - 1017
LID - 10.12688/f1000research.124273.1 [doi]
LID - 1017
AB  - Background: Dictyostelium discoideum is a ~10 microm diameter unicellular eukaryote 
      that lives on soil surfaces. When starved, D. discoideum cells aggregate into 
      streams of cells in a process called chemotaxis. In this report, we studied D. 
      discoideum cells during chemotaxis using 3D - mass spectrometry imaging (3D-MSI). 
      Methods: The 3D-MSI consisted of the sequential generation of 2D molecular maps 
      using burst alignment coupled to delayed extraction time-of flight secondary ion 
      mass spectrometry (TOF-SIMS) combined with a soft sputtering beam to access the 
      different layers. Results: Molecular maps with sub-cellular high spatial 
      resolution (~300 nm) indicated the presence of ions at m/z = 221 and 236 at the 
      front and sides, but reduced levels at the back, of cells moving toward of 
      aggregation streams. The 3D-MSI also detected an ion at m/z = 240 at the edges 
      and back, but reduced levels at the front, of aggregating cells. Other ions 
      showed an even distribution across the cells. Conclusions: Together, these 
      results demonstrate the utility of sub-micron MSI to study eukaryotic chemotaxis.
CI  - Copyright: (c) 2022 Castellanos A et al.
FAU - Castellanos, Anthony
AU  - Castellanos A
AD  - Chemistry and Biochemistry, Florida International University, Miami, Florida, 
      33199, USA.
FAU - Gomer, Richard H
AU  - Gomer RH
AUID- ORCID: 0000-0003-2361-4307
AD  - Biology, Texas A&M University, College Station, Texas, 77843-3474, USA.
FAU - Fernandez-Lima, Francisco
AU  - Fernandez-Lima F
AUID- ORCID: 0000-0002-1283-4390
AD  - Chemistry and Biochemistry, Florida International University, Miami, Florida, 
      33199, USA.
AD  - Biomolecular Science Institute, Florida International University, Miami, Florida, 
      33199, USA.
LA  - eng
SI  - figshare/10.6084/m9.figshare.20346711.v1
GR  - R35 GM139486/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20220908
PL  - England
TA  - F1000Res
JT  - F1000Research
JID - 101594320
RN  - 0 (Ions)
SB  - IM
MH  - *Dictyostelium
MH  - Spectrometry, Mass, Secondary Ion/methods
MH  - Imaging, Three-Dimensional
MH  - Eukaryotic Cells
MH  - Ions
PMC - PMC9947426
OTO - NOTNLM
OT  - Dictyostelium
OT  - aggregation
OT  - chemoattraction
OT  - mass spectrometry imaging
OT  - molecular imaging
COIS- No competing interests were disclosed.
EDAT- 2023/02/28 06:00
MHDA- 2023/03/03 06:00
PMCR- 2022/09/08
CRDT- 2023/02/27 05:04
PHST- 2022/08/17 00:00 [accepted]
PHST- 2023/02/27 05:04 [entrez]
PHST- 2023/02/28 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2022/09/08 00:00 [pmc-release]
AID - 10.12688/f1000research.124273.1 [doi]
PST - epublish
SO  - F1000Res. 2022 Sep 8;11:1017. doi: 10.12688/f1000research.124273.1. eCollection 
      2022.