PMID- 36845520
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230228
IS  - 2590-1575 (Electronic)
IS  - 2590-1575 (Linking)
VI  - 17
DP  - 2023 Mar 30
TI  - Investigation of the differences in the effect of (-)-epigallocatechin gallate 
      and proanthocyanidins on the functionality and allergenicity of soybean protein 
      isolate.
PG  - 100566
LID - 10.1016/j.fochx.2023.100566 [doi]
LID - 100566
AB  - In this study, the differences in effects of (-)-epigallocatechin gallate (EGCG) 
      and proanthocyanidins (PC) on the functionality and allergenicity of soybean 
      protein isolate (SPI) were studied. SDS-PAGE demonstrated that SPI-PC conjugates 
      exhibited more high-molecular-weight polymers (>180 kDa) than SPI-EGCG 
      conjugates. Structural analysis showed that SPI-PC conjugates exhibited more 
      disordered structures and protein-unfolding, improving the accessibility of PC to 
      modify SPI, compared to SPI-EGCG conjugates. LC/MS-MS demonstrated that PC caused 
      more modification of SPI and major soybean allergens than EGCG, resulting in a 
      lower abundance of epitopes. The successful attachment of EGCG and PC to SPI 
      significantly increased antioxidant capacity in conjugates. Furthermore, SPI-PC 
      conjugates exhibited greater emulsifying activity and lower immunoglobulin E 
      (IgE) binding capacity than SPI-EGCG conjugates, which was attributed to more 
      disordered structure and protein-unfolding in SPI-PC conjugates. It is implied 
      that proanthocyanidins may be promising compounds to interact with soybean 
      proteins to produce functional and hypoallergenic foods.
CI  - (c) 2023 The Authors.
FAU - Pi, Xiaowen
AU  - Pi X
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
FAU - Liu, Jiafei
AU  - Liu J
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
FAU - Sun, Yuxue
AU  - Sun Y
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
AD  - Key Laboratory of Soybean Biology of Chinese Education Ministry, Harbin, 
      Heilongjiang 150030, China.
FAU - Sun, Xiaomeng
AU  - Sun X
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
FAU - Sun, Zhigang
AU  - Sun Z
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
FAU - Cheng, Jianjun
AU  - Cheng J
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
FAU - Guo, Mingruo
AU  - Guo M
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
AD  - Department of Nutrition and Food Science, College of Agriculture and Life 
      Sciences, University of Vermont, Burlington 05405, United States.
LA  - eng
PT  - Journal Article
DEP - 20230107
PL  - Netherlands
TA  - Food Chem X
JT  - Food chemistry: X
JID - 101751436
PMC - PMC9945447
OTO - NOTNLM
OT  - (-)-epigallocatechin gallate
OT  - Functional properties
OT  - IgE binding capacity
OT  - Proanthocyanidins
OT  - Soybean protein isolate
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2023/02/28 06:00
MHDA- 2023/02/28 06:01
PMCR- 2023/01/07
CRDT- 2023/02/27 05:11
PHST- 2022/08/02 00:00 [received]
PHST- 2022/12/24 00:00 [revised]
PHST- 2023/01/05 00:00 [accepted]
PHST- 2023/02/27 05:11 [entrez]
PHST- 2023/02/28 06:00 [pubmed]
PHST- 2023/02/28 06:01 [medline]
PHST- 2023/01/07 00:00 [pmc-release]
AID - S2590-1575(23)00008-1 [pii]
AID - 100566 [pii]
AID - 10.1016/j.fochx.2023.100566 [doi]
PST - epublish
SO  - Food Chem X. 2023 Jan 7;17:100566. doi: 10.1016/j.fochx.2023.100566. eCollection 
      2023 Mar 30.