PMID- 36846309
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230228
IS  - 2590-0064 (Electronic)
IS  - 2590-0064 (Linking)
VI  - 19
DP  - 2023 Apr
TI  - Human urine-derived stem cell exosomes delivered via injectable GelMA templated 
      hydrogel accelerate bone regeneration.
PG  - 100569
LID - 10.1016/j.mtbio.2023.100569 [doi]
LID - 100569
AB  - The key to critical bone regeneration in tissue engineering relies on an ideal 
      bio-scaffold coated with a controlled release of growth factors. Gelatin 
      methacrylate (GelMA) and Hyaluronic acid methacrylate (HAMA) have been a novel 
      topic of interest in bone regeneration while introducing appropriate 
      nano-hydroxyapatite (nHAP) to improve its mechanical properties. And the exosomes 
      derived from human urine-derived stem cells (human (USC)EXOs) have also been 
      reported to promote osteogenesis in tissue engineering. The present study aimed 
      to design a new GelMA-HAMA/nHAP composite hydrogel as a drug delivery system. The 
      (USC)EXOs were encapsulated and slow-released in the hydrogel for better 
      osteogenesis. The characterization of the GelMA-based hydrogel showed excellent 
      controlled release performance and appropriate mechanical properties. The in 
      vitro studies showed that the (USC)EXOs/GelMA-HAMA/nHAP composite hydrogel could 
      promote the osteogenesis of bone marrow mesenchymal stem cells (BMSCs) and the 
      angiogenesis of endothelial progenitor cells (EPCs), respectively. Meanwhile, the 
      in vivo results confirmed that this composite hydrogel could significantly 
      promote the defect repair of cranial bone in the rat model. In addition, we also 
      found that (USC)EXOs/GelMA-HAMA/nHAP composite hydrogel can promote the formation 
      of H-type vessels in the bone regeneration area, enhancing the therapeutic 
      effect. In conclusion, our findings suggested that this controllable and 
      biocompatible (USC)EXOs/GelMA-HAMA/nHAP composite hydrogel may effectively 
      promote bone regeneration by coupling osteogenesis and angiogenesis.
CI  - (c) 2023 The Authors.
FAU - Lu, Wei
AU  - Lu W
AD  - Department of Orthopedic Surgery, National Clinical Research Center of Geriatric 
      Disorders, Xiangya Hospital, Central South University, Changsha, China.
AD  - Hunan Engineering Research Center of Biomedical Metal and Ceramic Implants, 
      Changsha, China.
FAU - Zeng, Min
AU  - Zeng M
AD  - Department of Orthopedic Surgery, National Clinical Research Center of Geriatric 
      Disorders, Xiangya Hospital, Central South University, Changsha, China.
AD  - Hunan Engineering Research Center of Biomedical Metal and Ceramic Implants, 
      Changsha, China.
FAU - Liu, Wenbin
AU  - Liu W
AD  - Department of Orthopedic Surgery, National Clinical Research Center of Geriatric 
      Disorders, Xiangya Hospital, Central South University, Changsha, China.
AD  - Hunan Engineering Research Center of Biomedical Metal and Ceramic Implants, 
      Changsha, China.
FAU - Ma, Tianliang
AU  - Ma T
AD  - Department of Orthopedic Surgery, National Clinical Research Center of Geriatric 
      Disorders, Xiangya Hospital, Central South University, Changsha, China.
AD  - Hunan Engineering Research Center of Biomedical Metal and Ceramic Implants, 
      Changsha, China.
FAU - Fan, Xiaolei
AU  - Fan X
AD  - Department of Orthopedics, Honghui Hospital, Xi'an Jiaotong University, Xi'an, 
      China.
FAU - Li, Hui
AU  - Li H
AD  - Department of Orthopedics, The First Affiliated Hospital, Medical College of 
      Zhejiang University, Hangzhou, China.
FAU - Wang, Yinan
AU  - Wang Y
AD  - Department of Orthopedic Surgery, National Clinical Research Center of Geriatric 
      Disorders, Xiangya Hospital, Central South University, Changsha, China.
AD  - Hunan Engineering Research Center of Biomedical Metal and Ceramic Implants, 
      Changsha, China.
FAU - Wang, Haoyi
AU  - Wang H
AD  - Department of Orthopedic Surgery, National Clinical Research Center of Geriatric 
      Disorders, Xiangya Hospital, Central South University, Changsha, China.
AD  - Hunan Engineering Research Center of Biomedical Metal and Ceramic Implants, 
      Changsha, China.
FAU - Hu, Yihe
AU  - Hu Y
AD  - Department of Orthopedic Surgery, National Clinical Research Center of Geriatric 
      Disorders, Xiangya Hospital, Central South University, Changsha, China.
AD  - Department of Orthopedics, The First Affiliated Hospital, Medical College of 
      Zhejiang University, Hangzhou, China.
FAU - Xie, Jie
AU  - Xie J
AD  - Department of Orthopedic Surgery, National Clinical Research Center of Geriatric 
      Disorders, Xiangya Hospital, Central South University, Changsha, China.
AD  - Department of Orthopedics, The First Affiliated Hospital, Medical College of 
      Zhejiang University, Hangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20230201
PL  - England
TA  - Mater Today Bio
JT  - Materials today. Bio
JID - 101757228
PMC - PMC9945756
OTO - NOTNLM
OT  - Bone regeneration
OT  - Exosomes
OT  - GelMA
OT  - H-type vessels
OT  - HAMA
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2023/02/28 06:00
MHDA- 2023/02/28 06:01
PMCR- 2023/02/01
CRDT- 2023/02/27 05:47
PHST- 2022/11/06 00:00 [received]
PHST- 2023/01/15 00:00 [revised]
PHST- 2023/01/29 00:00 [accepted]
PHST- 2023/02/27 05:47 [entrez]
PHST- 2023/02/28 06:00 [pubmed]
PHST- 2023/02/28 06:01 [medline]
PHST- 2023/02/01 00:00 [pmc-release]
AID - S2590-0064(23)00029-7 [pii]
AID - 100569 [pii]
AID - 10.1016/j.mtbio.2023.100569 [doi]
PST - epublish
SO  - Mater Today Bio. 2023 Feb 1;19:100569. doi: 10.1016/j.mtbio.2023.100569. 
      eCollection 2023 Apr.