PMID- 36851679
OWN - NLM
STAT- MEDLINE
DCOM- 20230301
LR  - 20230410
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 15
IP  - 2
DP  - 2023 Feb 7
TI  - Global Lipidome Profiling Revealed Multifaceted Role of Lipid Species in 
      Hepatitis C Virus Replication, Assembly, and Host Antiviral Response.
LID - 10.3390/v15020464 [doi]
LID - 464
AB  - Hepatitis C virus (HCV) is a major human pathogen that requires a better 
      understanding of its interaction with host cells. There is a close association of 
      HCV life cycle with host lipid metabolism. Lipid droplets (LDs) have been found 
      to be crucial organelles that support HCV replication and virion assembly. In 
      addition to their role in replication, LDs also have protein-mediated antiviral 
      properties that are activated during HCV infection. Studies have shown that HCV 
      replicates well in cholesterol and sphingolipid-rich membranes, but the ways in 
      which HCV alters host cell lipid dynamics are not yet known. In this study, we 
      performed a kinetic study to check the enrichment of LDs at different time points 
      of HCV infection. Based on the LD enrichment results, we selected early and later 
      time points of HCV infection for global lipidomic study. Early infection 
      represents the window period for HCV sensing and host immune response while later 
      infection represents the establishment of viral RNA replication, virion assembly, 
      and egress. We identified the dynamic profile of lipid species at early and later 
      time points of HCV infection by global lipidomic study using mass spectrometry. 
      At early HCV infection, phosphatidylinositol phospholipids (PIPs), 
      lysophosphatidic acid (LPA), triacyl glycerols (TAG), phosphatidylcholine (PC), 
      and trihexosylceramides (Hex3Cer) were observed to be enriched. Similarly, free 
      fatty acids (FFA), phosphatidylethanolamine (PE), N-acylphosphatidylethanolamines 
      (NAPE), and tri acylglycerols were enriched at later time points of HCV 
      infection. Lipids enriched at early time of infection may have role in HCV 
      sensing, viral attachment, and immune response as LPA and PIPs are important for 
      immune response and viral attachment, respectively. Moreover, lipid species 
      observed at later infection may contribute to HCV replication and virion assembly 
      as PE, FFA, and triacylglycerols are known for the similar function. In 
      conclusion, we identified lipid species that exhibited dynamic profile across 
      early and later time points of HCV infection compared to mock cells, which could 
      be therapeutically relevant in the design of more specific and effective 
      anti-viral therapies.
FAU - Islam, Khursheed Ul
AU  - Islam KU
AD  - Multidisciplinary Center for Advanced Research and Studies, Jamia Millia Islamia, 
      Jamia Nagar, New Delhi 110025, India.
FAU - Anwar, Saleem
AU  - Anwar S
AD  - Multidisciplinary Center for Advanced Research and Studies, Jamia Millia Islamia, 
      Jamia Nagar, New Delhi 110025, India.
FAU - Patel, Ayyub A
AU  - Patel AA
AUID- ORCID: 0000-0002-5320-3202
AD  - Department of Clinical Biochemistry, College of Medicine, King Khalid University, 
      Abha 62529, Saudi Arabia.
FAU - Mirdad, Mohammed Tarek
AU  - Mirdad MT
AD  - College of Medicine, King Khalid University, Abha 62529, Saudi Arabia.
FAU - Mirdad, Mahmoud Tarek
AU  - Mirdad MT
AD  - College of Medicine, King Khalid University, Abha 62529, Saudi Arabia.
FAU - Azmi, Md Iqbal
AU  - Azmi MI
AD  - Multidisciplinary Center for Advanced Research and Studies, Jamia Millia Islamia, 
      Jamia Nagar, New Delhi 110025, India.
FAU - Ahmad, Tanveer
AU  - Ahmad T
AD  - Multidisciplinary Center for Advanced Research and Studies, Jamia Millia Islamia, 
      Jamia Nagar, New Delhi 110025, India.
FAU - Fatima, Zeeshan
AU  - Fatima Z
AD  - Department of Medical Laboratory Sciences, College of Applied Medical Sciences, 
      University of Bisha, Bisha 61922, Saudi Arabia.
AD  - Amity Institute of Biotechnology, Amity University Haryana, Manesar, Gurugram 
      122413, India.
FAU - Iqbal, Jawed
AU  - Iqbal J
AD  - Multidisciplinary Center for Advanced Research and Studies, Jamia Millia Islamia, 
      Jamia Nagar, New Delhi 110025, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230207
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Antiviral Agents)
RN  - PDC6A3C0OX (Glycerol)
SB  - IM
MH  - Humans
MH  - *Hepacivirus
MH  - Lipidomics
MH  - *Hepatitis C
MH  - Antiviral Agents/pharmacology
MH  - Glycerol
PMC - PMC9965260
OTO - NOTNLM
OT  - antiviral response
OT  - fatty acids
OT  - glycerolipids
OT  - glycerophospholipids
OT  - hepatitis C virus
OT  - lipidome
COIS- Authors have declared no conflict of interest.
EDAT- 2023/03/01 06:00
MHDA- 2023/03/03 06:00
PMCR- 2023/02/07
CRDT- 2023/02/28 01:41
PHST- 2022/12/20 00:00 [received]
PHST- 2023/01/29 00:00 [revised]
PHST- 2023/02/01 00:00 [accepted]
PHST- 2023/02/28 01:41 [entrez]
PHST- 2023/03/01 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2023/02/07 00:00 [pmc-release]
AID - v15020464 [pii]
AID - viruses-15-00464 [pii]
AID - 10.3390/v15020464 [doi]
PST - epublish
SO  - Viruses. 2023 Feb 7;15(2):464. doi: 10.3390/v15020464.