PMID- 36852700
OWN - NLM
STAT- MEDLINE
DCOM- 20230307
LR  - 20230319
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Print)
IS  - 0963-6897 (Linking)
VI  - 32
DP  - 2023 Jan-Dec
TI  - Hypermethylation-Mediated lncRNA MAGI2-AS3 Downregulation Facilitates Malignant 
      Progression of Laryngeal Squamous Cell Carcinoma via Interacting With SPT6.
PG  - 9636897231154574
LID - 10.1177/09636897231154574 [doi]
LID - 09636897231154574
AB  - Long noncoding RNAs (lncRNAs) have an effect on the occurrence and progression of 
      a considerable number of diseases, especially cancer. Existing research has 
      suggested that MAGI2 antisense RNA 3 (MAGI2-AS3) takes on a critical significance 
      in the development of hepatocellular carcinoma and lung cancer. However, the 
      functions of MAGI2-AS3 in laryngeal squamous cell carcinoma (LSCC) remain 
      unclear. In this study, MAGI2-AS3 expression level in LSCC tissue and cell lines 
      was detected, and the effect of MAGI2-AS3 overexpressed on LSCC phenotypes and 
      the possible influence mechanisms were examined. MAGI2-AS3 was downregulated in 
      the tissues of LSCC patients versus non-tumor tissues, and it was correlated with 
      advanced TNM (tumor, node, metastasis) stage and lymph node metastases, as 
      indicated by the results of this study. MAGI2-AS3 inhibited the proliferation, 
      migration, and invasion of LSCC cells in vitro and in vivo. Furthermore, the 
      hypermethylation level of the MAGI2-AS3 promoter region was indicated by 
      bisulfite genomic sequencing and methylation-specific polymerase chain reaction, 
      such that MAGI2-AS3 expression was downregulated. Besides, MAGI2-AS3 promoter 
      hypermethylation was regulated by DNA methyltransferase 1 (DNMT1), and MAGI2-AS3 
      expression was reversed by 5-Aza-2'-deoxycytidine (5-Aza). Moreover, the result 
      of the RNA pull-down experiment suggested that 38 proteins were enriched in the 
      MAGI2-AS3 group versus the control group in TU177 cells. To be specific, SPT6 
      (ie, a conserved protein) was enriched by fold change >10. SPT6 knockdown reduced 
      the antitumor effect of MAGI2-AS3 in TU177 and AMC-HN-8 cells. Meanwhile, SPT6 
      overexpression inhibited the proliferation, metastasis, and invasion of TU177 and 
      AMC-HN-8 cells. As revealed by the above findings, DNMT1-regulated MAGI2-AS3 
      promoter hypermethylation led to downregulated MAGI2-AS3 expression, such that 
      the presence and progression of LSCC were inhibited in an SPT6 binding-dependent 
      manner.
FAU - Wang, Jiantao
AU  - Wang J
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, China.
FAU - Yang, Chuan
AU  - Yang C
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, China.
FAU - Cao, Huan
AU  - Cao H
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, China.
FAU - Yang, Jianwang
AU  - Yang J
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, China.
FAU - Meng, Wenxia
AU  - Meng W
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, China.
FAU - Yu, Miaomiao
AU  - Yu M
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, China.
FAU - Yu, Lei
AU  - Yu L
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, China.
FAU - Wang, Baoshan
AU  - Wang B
AUID- ORCID: 0000-0002-2455-8338
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 776B62CQ27 (Decitabine)
RN  - EC 2.7.4.8 (Guanylate Kinases)
RN  - EC 2.7.4.8 (MAGI2 protein, human)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (SUPT6H protein, human)
SB  - IM
MH  - Humans
MH  - Adaptor Proteins, Signal Transducing/genetics
MH  - Decitabine
MH  - Down-Regulation/genetics
MH  - Guanylate Kinases
MH  - *Head and Neck Neoplasms
MH  - *Liver Neoplasms
MH  - *RNA, Long Noncoding/genetics
MH  - Squamous Cell Carcinoma of Head and Neck
PMC - PMC9986895
OTO - NOTNLM
OT  - DNA methylation
OT  - MAGI2-AS3
OT  - SPT6
OT  - biomarker
OT  - laryngeal squamous cell carcinoma
COIS- The author(s) declared no potential conflicts of interest with respect to the 
      research, authorship, and/or publication of this article.
EDAT- 2023/03/01 06:00
MHDA- 2023/03/03 06:00
PMCR- 2023/02/28
CRDT- 2023/02/28 05:14
PHST- 2023/02/28 05:14 [entrez]
PHST- 2023/03/01 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2023/02/28 00:00 [pmc-release]
AID - 10.1177_09636897231154574 [pii]
AID - 10.1177/09636897231154574 [doi]
PST - ppublish
SO  - Cell Transplant. 2023 Jan-Dec;32:9636897231154574. doi: 
      10.1177/09636897231154574.