PMID- 36852887
OWN - NLM
STAT- MEDLINE
DCOM- 20230602
LR  - 20240229
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 192
IP  - 2
DP  - 2023 May 31
TI  - Altered expression of SELF-PRUNING disrupts homeostasis and facilitates signal 
      delivery to meristems.
PG  - 1517-1531
LID - 10.1093/plphys/kiad126 [doi]
AB  - Meristem maintenance, achieved through the highly conserved CLAVATA-WUSCHEL 
      (CLV-WUS) regulatory circuit, is fundamental in balancing stem cell proliferation 
      with cellular differentiation. Disruptions to meristem homeostasis can alter 
      meristem size, leading to enlarged organs. Cotton (Gossypium spp.), the world's 
      most important fiber crop, shows inherent variation in fruit size, presenting 
      opportunities to explore the networks regulating meristem homeostasis and to 
      impact fruit size and crop value. We identified and characterized the cotton 
      orthologs of genes functioning in the CLV-WUS circuit. Using virus-based gene 
      manipulation in cotton, we altered the expression of each gene to perturb 
      meristem regulation and increase fruit size. Targeted alteration of individual 
      components of the CLV-WUS circuit modestly fasciated flowers and fruits. 
      Unexpectedly, controlled expression of meristem regulator SELF-PRUNING (SP) 
      increased the impacts of altered CLV-WUS expression on flower and fruit 
      fasciation. Meristem transcriptomics showed SP and genes of the CLV-WUS circuit 
      are expressed independently from each other, suggesting these gene products are 
      not acting in the same path. Virus-induced silencing of GhSP facilitated the 
      delivery of other signals to the meristem to alter organ specification. SP has a 
      role in cotton meristem homeostasis, and changes in GhSP expression increased 
      access of virus-derived signals to the meristem.
CI  - (c) American Society of Plant Biologists 2023. All rights reserved. For 
      permissions, please e-mail: journals.permissions@oup.com.
FAU - McGarry, Roisin C
AU  - McGarry RC
AUID- ORCID: 0000-0002-8832-8080
AD  - Department of Biological Sciences and BioDiscovery Institute, University of North 
      Texas, Denton, TX 76203-5017, USA.
FAU - Kaur, Harmanpreet
AU  - Kaur H
AUID- ORCID: 0000-0002-3032-8387
AD  - Department of Biological Sciences and BioDiscovery Institute, University of North 
      Texas, Denton, TX 76203-5017, USA.
FAU - Lin, Yen-Tung
AU  - Lin YT
AUID- ORCID: 0000-0002-3667-5072
AD  - Department of Biological Sciences and BioDiscovery Institute, University of North 
      Texas, Denton, TX 76203-5017, USA.
FAU - Puc, Guadalupe Lopez
AU  - Puc GL
AUID- ORCID: 0000-0003-2961-5511
AD  - Centro de Investigacion y Asistencia en Tecnologia y Diseno del Estado de 
      Jalisco, Biotecnologia Vegetal, subsede Sureste, 97302 Merida, Mexico.
FAU - Eshed Williams, Leor
AU  - Eshed Williams L
AUID- ORCID: 0000-0002-2766-2608
AD  - The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, 
      Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 
      Rehovot 76100, Israel.
FAU - van der Knaap, Esther
AU  - van der Knaap E
AUID- ORCID: 0000-0003-4963-7427
AD  - Center for Applied Genetic Technologies, College of Agricultural and 
      Environmental Sciences, University of Georgia, Athens, GA 30602, USA.
FAU - Ayre, Brian G
AU  - Ayre BG
AUID- ORCID: 0000-0002-7399-200X
AD  - Department of Biological Sciences and BioDiscovery Institute, University of North 
      Texas, Denton, TX 76203-5017, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Homeodomain Proteins)
SB  - IM
MH  - *Meristem/metabolism
MH  - *Arabidopsis Proteins/metabolism
MH  - Flowers/genetics/metabolism
MH  - Fruit/genetics/metabolism
MH  - Homeostasis
MH  - Gene Expression Regulation, Plant
MH  - Homeodomain Proteins/genetics
PMC - PMC10231363
COIS- Conflict of interest statement. None declared.
EDAT- 2023/03/01 06:00
MHDA- 2023/06/02 06:43
PMCR- 2024/02/28
CRDT- 2023/02/28 07:36
PHST- 2022/10/03 00:00 [received]
PHST- 2023/02/06 00:00 [accepted]
PHST- 2023/06/02 06:43 [medline]
PHST- 2023/03/01 06:00 [pubmed]
PHST- 2023/02/28 07:36 [entrez]
PHST- 2024/02/28 00:00 [pmc-release]
AID - 7059621 [pii]
AID - kiad126 [pii]
AID - 10.1093/plphys/kiad126 [doi]
PST - ppublish
SO  - Plant Physiol. 2023 May 31;192(2):1517-1531. doi: 10.1093/plphys/kiad126.