PMID- 36854397
OWN - NLM
STAT- MEDLINE
DCOM- 20230302
LR  - 20230302
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 40
IP  - 3
DP  - 2023 Mar 10
TI  - [Application of fluorescence in situ hybridization technique to verify the 
      clonalities of non-clonal cytogenetic abnormalities identified in Myelodysplastic 
      syndrome].
PG  - 257-262
LID - 10.3760/cma.j.cn511374-20220228-00137 [doi]
AB  - OBJECTIVE: To assess the value of fluorescence in situ hybridization (FISH) 
      technique for the verification of the clonalities of non-clonal cytogenetic 
      abnormalities (n-CCA) identified by conventional chromosome banding analysis 
      (CBA) in patients with Myelodysplastic syndrome (MDS). METHODS: Clinical data and 
      results of karyotyping and FISH assays for 91 patients of MDS with n-CCA 
      identified by CBA were retrospectively analyzed. In total 94 non-clonal +8, 5q-, 
      -7/7q- or 20q- were detected by CBA, among which 43 (45.7%) were verified to be 
      clonal abnormalities by FISH. RESULTS: The detection rates for +8, 5q-, -7/7q- 
      and 20q- by FISH were 47.6% (30/63), 25% (2/8), 41.7% (5/12), 40% (2/5) and 66.7% 
      (4/6), respectively, with the positive cells accounting for 4% to 90% of all 
      counted cells, with a median value of 7%. The 91 patients were divided into three 
      groups including >/= 20, 10 ~< 20 and < 10 based on the numbers of metaphase cells 
      in CBA, and the detection rates by FISH for the three groups were 43.7% (31/71), 
      33.3% (3/9) and 63.6% (7/11), respectively, which showed no statistically 
      difference (P > 0.05). Continuous CBA and FISH surveys were conducted for 26 
      patients who received supportive treatment, and the results revealed that 91.7% 
      (11/12) of FISH-verified positive abnormalities had persisted, whereas 92.9% 
      (13/14) of the n-CCA verified as negative by FISH was transient. CONCLUSION: 
      Nearly half of the CBA identified n-CCA have been verified as clonal aberrations 
      by FISH, and the FISH detection rate showed no correlation with the number of 
      metaphase cells. FISH test is strongly recommended for verifying the clonalities 
      of n-CCA detected by CBA, and continuous cytogenetic survey of the patients with 
      MDS is necessary.
FAU - Wang, Zheng
AU  - Wang Z
AD  - Peking University Institute of Hematology, National Clinical Research Center for 
      Hematologic Disease, Beijing Key Laboratory of Hematopoietic Stem Cell 
      Transplantation, Peking University People's Hospital, Beijing 100044, China. 
      laiyueyun1008@sina.com.
FAU - Wang, Yanlin
AU  - Wang Y
FAU - Song, Wenjie
AU  - Song W
FAU - Feng, Lin
AU  - Feng L
FAU - Gao, Lu
AU  - Gao L
FAU - Li, Ye
AU  - Li Y
FAU - Huang, Xiaojun
AU  - Huang X
FAU - Lai, Yueyun
AU  - Lai Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Retrospective Studies
MH  - *Chromosome Aberrations
MH  - Karyotyping
MH  - *Myelodysplastic Syndromes/genetics
EDAT- 2023/03/01 06:00
MHDA- 2023/03/03 06:00
CRDT- 2023/02/28 19:23
PHST- 2023/02/28 19:23 [entrez]
PHST- 2023/03/01 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
AID - 940640049 [pii]
AID - 10.3760/cma.j.cn511374-20220228-00137 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2023 Mar 10;40(3):257-262. doi: 
      10.3760/cma.j.cn511374-20220228-00137.