PMID- 36858416
OWN - NLM
STAT- MEDLINE
DCOM- 20230303
LR  - 20230303
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 48
IP  - 2
DP  - 2023 Feb 25
TI  - [Effects of different acupuncture and moxibustion methods on articular cartilage 
      morphology and NF-kappaB p65/NLRP3 pathway in rats with knee osteoarthritis].
PG  - 185-91
LID - 10.13702/j.1000-0607.20220246 [doi]
AB  - OBJECTIVE: To compare the effects of filiform needling, electroacupuncture (EA) 
      and moxibustion on articular cartilage morphology and nuclear transcription 
      factor-kappaB (NF-kappaB) p65/ Nod-like receptor protein 3 (NLRP3) pathway in rats with 
      knee osteoarthritis (KOA). METHODS: Wistar rats were randomly divided into blank 
      control， model, filiform needling, EA and moxibustion groups, with 8 rats in each 
      group. The KOA model was established by injecting sodium iodoacetate into the 
      right knee cavity. Filiform needling, EA and moxibustion groups were treated with 
      the right side of "Dubi" (ST35) and "Neixiyan" (EX-LE5), and were given filiform 
      needling, EA and mild moxibustion therapies for 15 min respectively, once every 
      other day, for a total of 4 weeks. The diameter of the right knee joint was 
      observed. The ultrastructure of knee chondrocytes was observed by transmission 
      electron microscope. The contents of serum tumor necrosis factor-alpha (TNF-alpha), 
      interleukin (IL)-1beta and IL-18 were determined by ELISA. The expression levels of 
      NF-kappaB p65, NLRP3, apoptosis-related speckle like protein (ASC) and TNF-alpha in knee 
      cartilage were dectected by Western blot. RESULTS: Compared with the blank 
      control group, the right knee joint cavity was narrowed, chondrocytes were 
      constricted significantly, mitochondria were moderately swollen, the diameter of 
      the right knee was increased, and the contents of serum TNF-alpha, IL-1beta and IL-18 
      were increased (P<0.01), the expressions of NF-kappaB p65, NLRP3 and TNF-alpha in knee 
      cartilage were increased (P<0.01) while the expression of ASC was decreased 
      (P<0.01) in the model group.Compared with model group, the narrowed knee joint 
      cavity and chondrocyte injury were improved， knee diameter decreased (P<0.01, 
      P<0.05), and the content of serum TNF-alpha and the expression of NLRP3 in knee 
      cartilage were decreased (P<0.05, P<0.01) in 3 treatment groups; the contents of 
      serum IL-1beta and IL-18, and the expression of TNF-alpha in knee cartilage were 
      decreased (P<0.01, P<0.05), while the expression of ASC in knee cartilage was 
      increased (P<0.01) in EA and moxibustion groups; the expression of NF-kappaB p65 in 
      knee cartilage was decreased (P<0.01) in moxibustion group. Compared with EA 
      group, the content of serum IL-18 was decreased (P<0.05) in moxibustion group. 
      CONCLUSION: Filiform needling, EA and moxibustion can all reduce the formation of 
      knee osteophytosis in KOA rats, alleviate joint cavity narrowing, improve the 
      ultrastructure of articular cartilage, reduce the level of inflammatory factors, 
      regulate the NF-kappaB p65/NLRP3 pathway. Moxibustion has the most obvious regulatory 
      effect among the 3 treatment methods.
FAU - Zhang, Xiao-Qin
AU  - Zhang XQ
AD  - Institute of Acupuncture Moxibustion and Tuina, Hebei University of Chinese 
      Medicine, Shijiazhuang 050200, China.
FAU - Li, Qing
AU  - Li Q
AD  - Hebei Key Laboratory of Acupuncture and Moxibustion Specificity Effects, 
      Shijiazhuang 050091.
FAU - Xue, Ping-Ju
AU  - Xue PJ
AD  - Institute of Acupuncture Moxibustion and Tuina, Hebei University of Chinese 
      Medicine, Shijiazhuang 050200, China.
FAU - Jia, Ye-Juan
AU  - Jia YJ
AD  - Institute of Acupuncture Moxibustion and Tuina, Hebei University of Chinese 
      Medicine, Shijiazhuang 050200, China.
FAU - Xing, Hai-Jiao
AU  - Xing HJ
AD  - Hebei Key Laboratory of Acupuncture and Moxibustion Specificity Effects, 
      Shijiazhuang 050091.
FAU - Xu, Jing
AU  - Xu J
AD  - Institute of Acupuncture Moxibustion and Tuina, Hebei University of Chinese 
      Medicine, Shijiazhuang 050200, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
RN  - 0 (NF-kappa B)
RN  - 0 (Interleukin-18)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Nlrp3 protein, rat)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Moxibustion
MH  - *Cartilage, Articular
MH  - *Osteoarthritis, Knee
MH  - NF-kappa B
MH  - Interleukin-18
MH  - NLR Family, Pyrin Domain-Containing 3 Protein
MH  - Tumor Necrosis Factor-alpha
MH  - Rats, Wistar
MH  - *Acupuncture Therapy
OTO - NOTNLM
OT  - Electroacupuncture
OT  - Filiform needling
OT  - Inflammatory factor
OT  - Knee osteoarthritis
OT  - Moxibustion
OT  - Nuclear transcription factor-kappaB p65/Nod-like receptor protein 3 pathway
EDAT- 2023/03/02 06:00
MHDA- 2023/03/04 06:00
CRDT- 2023/03/01 20:25
PHST- 2023/03/01 20:25 [entrez]
PHST- 2023/03/02 06:00 [pubmed]
PHST- 2023/03/04 06:00 [medline]
AID - 10.13702/j.1000-0607.20220246 [doi]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2023 Feb 25;48(2):185-91. doi: 10.13702/j.1000-0607.20220246.