PMID- 36858599
OWN - NLM
STAT- MEDLINE
DCOM- 20230526
LR  - 20230526
IS  - 1884-2836 (Electronic)
IS  - 1344-6304 (Linking)
VI  - 76
IP  - 3
DP  - 2023 May 24
TI  - Ubiquitin-Specific Peptidase 8 Is Critical for the Onset of Infectious 
      Osteomyelitis by Targeting RIPK2 Ubiquitination.
PG  - 197-203
LID - 10.7883/yoken.JJID.2022.515 [doi]
AB  - Receptor-interacting serine/threonine kinase (RIPK) is associated with cellular 
      inflammation and immune regulation. The current study explored the role of RIPK2 
      in osteomyelitis and the potential upstream targets of RIPK2. A Staphylococcus 
      aureus-induced osteomyelitis mouse model was established using wild-type (WT) and 
      ubiquitin-specific peptidase 8 (USP8)-deficient (USP(-/-)) mice, and the 
      osteomyelitis-related symptoms were evaluated. Bone marrow-derived macrophages 
      (BMDMs) were isolated from the WT and USP(-/-) mice. Enzyme-linked immunosorbent 
      assays, quantitative polymerase chain reaction, and immunoblot analysis were used 
      to determine the levels of target biomarkers, which were induced by 
      lipopolysaccharide (LPS), CpG, or PAM3CSK4. USP8 promoted RIPK2-mediated NF-kappaB 
      activation. USP8 is indispensable for RIPK2-mediated LPS-induced NF-kappaB activation 
      in BMDMs. USP8 is required for the production of inflammatory cytokines induced 
      by LPS, CpG, or PAM3CSK4 in BMDMs. In addition, USP(-/-) mice exhibited 
      ameliorated symptoms, including less body weight and cortical bone loss, and 
      reduced bacterial load and reactive bone formation in the S. aureus-induced 
      osteomyelitis mouse model. USP8 is critical in the S. aureus-induced 
      osteomyelitis mouse model by targeting RIPK2 ubiquitination.
FAU - Chen, Yuanliang
AU  - Chen Y
AD  - Department of Orthopedic Surgery, Haikou Orthopedic and Diabetes Hospital of 
      Shanghai Sixth People's Hospital, China.
FAU - Wan, Yongbai
AU  - Wan Y
AD  - Department of Orthopedic Surgery, Haikou Orthopedic and Diabetes Hospital of 
      Shanghai Sixth People's Hospital, China.
FAU - Shan, Haojie
AU  - Shan H
AD  - Department of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to 
      Shanghai Jiao Tong University School of Medicine, China.
FAU - Lin, Yiwei
AU  - Lin Y
AD  - Department of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to 
      Shanghai Jiao Tong University School of Medicine, China.
FAU - Xia, Wenyang
AU  - Xia W
AD  - Department of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to 
      Shanghai Jiao Tong University School of Medicine, China.
FAU - Yin, Fuli
AU  - Yin F
AD  - Department of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to 
      Shanghai Jiao Tong University School of Medicine, China.
FAU - Jiang, Chaolai
AU  - Jiang C
AD  - Department of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to 
      Shanghai Jiao Tong University School of Medicine, China.
FAU - Shi, Zhongmin
AU  - Shi Z
AD  - Department of Orthopedic Surgery, Haikou Orthopedic and Diabetes Hospital of 
      Shanghai Sixth People's Hospital, China.
AD  - Department of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to 
      Shanghai Jiao Tong University School of Medicine, China.
LA  - eng
PT  - Journal Article
DEP - 20230228
PL  - Japan
TA  - Jpn J Infect Dis
JT  - Japanese journal of infectious diseases
JID - 100893704
RN  - 0 (NF-kappa B)
RN  - 0 (Lipopolysaccharides)
RN  - EC 3.4.19.12 (Ubiquitin-Specific Proteases)
RN  - EC 2.7.11.1 (Ripk2 protein, mouse)
RN  - EC 2.7.11.1 (Receptor-Interacting Protein Serine-Threonine Kinase 2)
SB  - IM
MH  - Mice
MH  - Animals
MH  - NF-kappa B
MH  - Lipopolysaccharides/pharmacology
MH  - Staphylococcus aureus
MH  - Ubiquitination
MH  - *Osteomyelitis
MH  - *Communicable Diseases
MH  - Ubiquitin-Specific Proteases/genetics
MH  - Receptor-Interacting Protein Serine-Threonine Kinase 2
OTO - NOTNLM
OT  - NF-kappaB
OT  - RIPK2
OT  - USP8
OT  - osteomyelitis
OT  - ubiquitination
EDAT- 2023/03/02 06:00
MHDA- 2023/05/26 06:42
CRDT- 2023/03/01 20:54
PHST- 2023/05/26 06:42 [medline]
PHST- 2023/03/02 06:00 [pubmed]
PHST- 2023/03/01 20:54 [entrez]
AID - 10.7883/yoken.JJID.2022.515 [doi]
PST - ppublish
SO  - Jpn J Infect Dis. 2023 May 24;76(3):197-203. doi: 10.7883/yoken.JJID.2022.515. 
      Epub 2023 Feb 28.