PMID- 36862211
OWN - NLM
STAT- MEDLINE
DCOM- 20230306
LR  - 20230310
IS  - 1432-072X (Electronic)
IS  - 0302-8933 (Linking)
VI  - 205
IP  - 3
DP  - 2023 Mar 2
TI  - Utilization of Bacillus cereus strain CGK5 associated with cow feces in the 
      degradation of commercially available high-density polyethylene (HDPE).
PG  - 101
LID - 10.1007/s00203-023-03448-5 [doi]
AB  - The accumulation and mismanagement of high-density polyethylene (HDPE) waste in 
      the environment is a complex problem in the present scenario. Biodegradation of 
      this thermoplastic polymer is a promising environmentally sustainable method that 
      offers a significant opportunity to address plastic waste management with minimal 
      negative repercussion on the environment. In this framework, HDPE-degrading 
      bacterium strain CGK5 was isolated from the fecal matter of cow. The 
      biodegradation efficiency of strain was assessed, including percentage reduction 
      in HDPE weight, cell surface hydrophobicity, extracellular biosurfactant 
      production, viability of surface adhered cells, as well as biomass in terms of 
      protein content. Through molecular techniques, strain CGK5 was identified as 
      Bacillus cereus. Significant weight loss of 1.83% was observed in the HDPE film 
      treated with strain CGK5 for 90 days. The FE-SEM analysis revealed the profused 
      bacterial growth which ultimately caused the distortions in HDPE films. 
      Furthermore, EDX study indicated a significant decrease in percentage carbon 
      content at atomic level, whereas FTIR analysis confirmed chemical groups' 
      transformation as well as an increment in the carbonyl index supposedly caused by 
      bacterial biofilm biodegradation. Our findings shed light on the ability of our 
      strain B. cereus CGK5 to colonize and use HDPE as a sole carbon source, 
      demonstrating its applicability for future eco-friendly biodegradation processes.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Gupta, Kartikey Kumar
AU  - Gupta KK
AD  - Department of Botany and Microbiology, Gurukula Kangri (Deemed to be University), 
      Uttarakhand, Haridwar, India.
FAU - Sharma, Kamal Kant
AU  - Sharma KK
AD  - Department of Botany and Microbiology, Gurukula Kangri (Deemed to be University), 
      Uttarakhand, Haridwar, India. kamal.sharma@gkv.ac.in.
FAU - Chandra, Harish
AU  - Chandra H
AD  - Department of Botany and Microbiology, Gurukula Kangri (Deemed to be University), 
      Uttarakhand, Haridwar, India.
LA  - eng
PT  - Journal Article
DEP - 20230302
PL  - Germany
TA  - Arch Microbiol
JT  - Archives of microbiology
JID - 0410427
RN  - 9002-88-4 (Polyethylene)
RN  - 7440-44-0 (Carbon)
RN  - 0 (Plastics)
SB  - IM
MH  - Animals
MH  - Female
MH  - Cattle
MH  - *Bacillus cereus/genetics
MH  - *Polyethylene
MH  - Biodegradation, Environmental
MH  - Carbon
MH  - Feces
MH  - Plastics
OTO - NOTNLM
OT  - Biodegradation
OT  - Biofilm
OT  - Biosurfactant
OT  - Cow dung
OT  - HDPE
OT  - Hydrophobicity
EDAT- 2023/03/03 06:00
MHDA- 2023/03/07 06:00
CRDT- 2023/03/02 11:15
PHST- 2022/11/03 00:00 [received]
PHST- 2023/02/21 00:00 [accepted]
PHST- 2022/12/13 00:00 [revised]
PHST- 2023/03/02 11:15 [entrez]
PHST- 2023/03/03 06:00 [pubmed]
PHST- 2023/03/07 06:00 [medline]
AID - 10.1007/s00203-023-03448-5 [pii]
AID - 10.1007/s00203-023-03448-5 [doi]
PST - epublish
SO  - Arch Microbiol. 2023 Mar 2;205(3):101. doi: 10.1007/s00203-023-03448-5.