PMID- 36863558 OWN - NLM STAT- MEDLINE DCOM- 20230411 LR - 20230411 IS - 1873-6351 (Electronic) IS - 0278-6915 (Linking) VI - 175 DP - 2023 May TI - Taurine reduces apoptosis mediated by endoplasmic reticulum stress in islet beta-cells induced by high-fat and -glucose diets. PG - 113700 LID - S0278-6915(23)00102-3 [pii] LID - 10.1016/j.fct.2023.113700 [doi] AB - Poor eating habits, especially high-fat and -glucose diets intake, can lead to endoplasmic reticulum (ER) stress in islet beta-cells, insulin resistance, and islet beta-cell dysfunction and cause islet beta-cell apoptosis, which leads to type 2 diabetes mellitus (T2DM). Taurine is a crucial amino acid in the human body. In this study, we aimed to explore the mechanism through which taurine reduces glycolipid toxicity. INS-1 islet beta-cell lines were cultured with a high concentration of fat and glucose. SD rats were fed a high-fat and -glucose diet. MTS, Transmission electron microscopy, Flow cytometry, Hematoxylin-eosin, TUNEL, Western blotting analysis and other methods were used to detect relevant indicators. The research found that taurine increases the cell activity, reduces the apoptosis rate, alleviates the structural changes of ER under high-fat and -glucose exposure models. In addition, taurine improves blood lipid content and islets pathological changes, regulates the relative protein expression in ER stress and apoptosis, increases the insulin sensitivity index (HOMA-IS), and reduces the insulin resistance index (HOMAC-IR) of SD rats fed with a high-fat and -glucose diet. CI - Copyright (c) 2023 Elsevier Ltd. All rights reserved. FAU - Zhao, Dongdong AU - Zhao D AD - Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: 2019200148@stu.syau.edu.cn. FAU - Zhang, Xiaozhou AU - Zhang X AD - Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: 2018240337@stu.syau.edu.cn. FAU - Bian, Yongxin AU - Bian Y AD - Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: 2018240352@stu.syau.edu.cn. FAU - Meng, Lu AU - Meng L AD - Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: 20162505@stu.syau.edu.cn. FAU - Wu, Yutong AU - Wu Y AD - Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: 2018220490@stu.syau.edu.cn. FAU - Ma, Yidi AU - Ma Y AD - Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: 2019220524@stu.syau.edu.cn. FAU - Li, Chen AU - Li C AD - Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: 2020220580@stu.syau.edu.cn. FAU - Wang, Jingjing AU - Wang J AD - Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: 2019240403@stu.syau.edu.cn. FAU - Fu, Zizheng AU - Fu Z AD - Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: 2020240601@stu.syau.edu.cn. FAU - Dai, Jingyuan AU - Dai J AD - Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: 2019240399@stu.syau.edu.cn. FAU - Yin, Deqi AU - Yin D AD - Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: 2018200150@stu.syau.edu.cn. FAU - Lin, Shumei AU - Lin S AD - Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: 2004500035@syau.edu.cn. FAU - Hu, Jianmin AU - Hu J AD - Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address: Hujianmin59@syau.edu.cn. LA - eng PT - Journal Article DEP - 20230228 PL - England TA - Food Chem Toxicol JT - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JID - 8207483 RN - IY9XDZ35W2 (Glucose) RN - 1EQV5MLY3D (Taurine) SB - IM MH - Rats MH - Humans MH - Animals MH - Glucose/metabolism MH - *Insulin Resistance MH - *Diabetes Mellitus, Type 2/metabolism MH - Taurine/pharmacology MH - *Insulin-Secreting Cells/pathology MH - Rats, Sprague-Dawley MH - Diet, High-Fat/adverse effects MH - Apoptosis MH - Endoplasmic Reticulum Stress OTO - NOTNLM OT - Apoptosis OT - Endoplasmic reticulum stress OT - Glucolipotoxicity OT - Islet beta-cells OT - Taurine OT - Type 2 diabetes mellitus COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2023/03/03 06:00 MHDA- 2023/04/11 06:41 CRDT- 2023/03/02 19:25 PHST- 2022/04/19 00:00 [received] PHST- 2023/02/14 00:00 [revised] PHST- 2023/02/27 00:00 [accepted] PHST- 2023/04/11 06:41 [medline] PHST- 2023/03/03 06:00 [pubmed] PHST- 2023/03/02 19:25 [entrez] AID - S0278-6915(23)00102-3 [pii] AID - 10.1016/j.fct.2023.113700 [doi] PST - ppublish SO - Food Chem Toxicol. 2023 May;175:113700. doi: 10.1016/j.fct.2023.113700. Epub 2023 Feb 28.