PMID- 36864443
OWN - NLM
STAT- MEDLINE
DCOM- 20230310
LR  - 20230310
IS  - 1479-5876 (Electronic)
IS  - 1479-5876 (Linking)
VI  - 21
IP  - 1
DP  - 2023 Mar 3
TI  - CD206(+) tumor-associated macrophages interact with CD4(+) tumor-infiltrating 
      lymphocytes and predict adverse patient outcome in human laryngeal squamous cell 
      carcinoma.
PG  - 167
LID - 10.1186/s12967-023-03910-4 [doi]
LID - 167
AB  - BACKGROUND: Tumor-associated macrophages (TAMs) are major component in the tumor 
      microenvironment (TME) and play regulatory role in tumor progression. We aimed to 
      investigate the infiltration and prognostic value of TAMs in laryngeal squamous 
      cell carcinoma (LSCC) and to reveal the underlying mechanism of TAM subgroups in 
      tumorigenesis. METHODS: Hematoxylin and eosin (HE) staining were performed to 
      define the tumor nest and stroma of LSCC tissue microarrays. CD206 + /CD163 + and 
      iNOS + TAM infiltrating profiles were obtained and analyzed through 
      double-labeling immunofluorescence and immunohistochemical staining. The 
      recurrence-free (RFS) and overall survival (OS) curves based on the infiltration 
      of TAMs were plotted using the Kaplan-Meier method. Infiltration of macrophages, 
      T lymphocytes and their corresponding subgroups were analyzed in fresh LSCC 
      tissue samples by flow cytometry. RESULTS: We found that CD206(+) rather than 
      CD163(+) M2-like TAMs were the most enriched population in the TME of human LSCC. 
      CD206(+) macrophages localized mostly in the tumor stroma (TS) rather than the 
      tumor nest (TN) region. In contrast, relatively low infiltration of iNOS(+) 
      M1-like TAMs were found in the TS and almost none in the TN region. High level of 
      TS CD206(+) TAM infiltration correlated with poor prognosis. Interestingly, we 
      identified a HLA-DR(high) CD206(+) macrophage subgroup that was significantly 
      associated with the tumor-infiltrating CD4(+) T lymphocytes and showed different 
      surface costimulatory molecule expression than that of the HLA-DR(low/)-CD206(+) 
      subgroup. Taken together, our results indicate that HLA-DR(high)-CD206(+) is a 
      highly activated subgroup of CD206 + TAMs that may interact with CD4 + T cells 
      through MHC-II axis and promote tumorigenesis.
CI  - (c) 2023. The Author(s).
FAU - Heng, Yu
AU  - Heng Y
AD  - ENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan 
      University, Shanghai, 200031, People's Republic of China.
FAU - Zhu, Xiaoke
AU  - Zhu X
AD  - ENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan 
      University, Shanghai, 200031, People's Republic of China.
FAU - Lin, Hanqing
AU  - Lin H
AD  - ENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan 
      University, Shanghai, 200031, People's Republic of China.
FAU - Jingyu, Ma
AU  - Jingyu M
AD  - ENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan 
      University, Shanghai, 200031, People's Republic of China.
FAU - Ding, Xuping
AU  - Ding X
AD  - Shanghai Institute of Immunology, Department of Immunology and Microbiology, 
      Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, People's 
      Republic of China.
FAU - Tao, Lei
AU  - Tao L
AD  - ENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, Fudan 
      University, Shanghai, 200031, People's Republic of China. lei.tao@fdeent.org.
FAU - Lu, Liming
AU  - Lu L
AD  - Shanghai Institute of Immunology, Department of Immunology and Microbiology, 
      Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, People's 
      Republic of China. youlanda2009@sjtu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230303
PL  - England
TA  - J Transl Med
JT  - Journal of translational medicine
JID - 101190741
RN  - 0 (MRC1 protein, human)
SB  - IM
MH  - Humans
MH  - Carcinogenesis
MH  - CD4-Positive T-Lymphocytes
MH  - Cell Transformation, Neoplastic
MH  - *Head and Neck Neoplasms
MH  - Lymphocytes, Tumor-Infiltrating
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Tumor Microenvironment
MH  - *Tumor-Associated Macrophages
PMC - PMC9983170
OTO - NOTNLM
OT  - CD4+ T cell interaction
OT  - Immunosuppressive microenvironment
OT  - Laryngeal squamous cell carcinoma
OT  - Macrophage infiltrating pattern
OT  - Prognosis
OT  - Tumor-associated macrophages
COIS- There is no competing interests.
EDAT- 2023/03/03 06:00
MHDA- 2023/03/07 06:00
PMCR- 2023/03/03
CRDT- 2023/03/02 23:34
PHST- 2022/02/09 00:00 [received]
PHST- 2023/01/21 00:00 [accepted]
PHST- 2023/03/02 23:34 [entrez]
PHST- 2023/03/03 06:00 [pubmed]
PHST- 2023/03/07 06:00 [medline]
PHST- 2023/03/03 00:00 [pmc-release]
AID - 10.1186/s12967-023-03910-4 [pii]
AID - 3910 [pii]
AID - 10.1186/s12967-023-03910-4 [doi]
PST - epublish
SO  - J Transl Med. 2023 Mar 3;21(1):167. doi: 10.1186/s12967-023-03910-4.