PMID- 36864471
OWN - NLM
STAT- MEDLINE
DCOM- 20230306
LR  - 20230306
IS  - 1749-799X (Electronic)
IS  - 1749-799X (Linking)
VI  - 18
IP  - 1
DP  - 2023 Mar 2
TI  - Effect study of exosomes derived from platelet-rich plasma in the treatment of 
      knee cartilage defects in rats.
PG  - 160
LID - 10.1186/s13018-023-03576-0 [doi]
LID - 160
AB  - BACKGROUND: The repair of articular cartilage defects has always been a difficult 
      problem. We aimed to investigate the therapeutic effect of intra-articular 
      injection of platelet-rich plasma (RPR) and PRP-derived exosomes (PRP-Exos) on 
      cartilage defects in rat knee joints and then provide experience for the use of 
      PRP-exos in cartilage defect repair. METHODS: Rat abdominal aortic blood was 
      collected, and PRP was extracted by two-step centrifugation. PRP-exos were 
      obtained by kit extraction, and PRP-exos were identified by various methods. 
      After the rats were anesthetized, a cartilage defect subchondral bone was created 
      at the proximal end of the origin of the femoral cruciate ligament with a drill. 
      SD rats were divided into 4 groups, including PRP group, 50 mug/ml PRP-exos group, 
      5 mug/ml PRP-exos group, and control group. One week after the operation, 50 mug/ml 
      PRP, 50 mug/ml PRP-exos, 5 mug/ml PRP-exos and normal saline were injected into the 
      knee joint cavity of rats in each group, once a week. A total of two injections 
      were given. On the 5th and 10th week after drug injection, the serum levels of 
      matrix metalloproteinase 3 (MMP-3) and tissue inhibitor of matrix 
      metalloproteinase 1 (TIMP-1) were detected by each treatment method, 
      respectively. The rats were killed at the 5th and 10th weeks, respectively, and 
      the cartilage defect repair was observed and scored. The defect repair tissue 
      sections were used for HE staining and type II collagen immunohistochemical 
      staining. RESULTS: The histological results showed that both PRP-exos and PRP 
      could promote cartilage defect repair and type II collagen formation, and the 
      promoting effect of PRP-exos was significantly better than that of PRP. In 
      addition, enzyme-linked immunosorbent assay (ELISA) results showed that compared 
      with PRP, PRP-exos could significantly increase serum TIMP-1 and decrease serum 
      MMP-3 in rats. And the promoting effect of PRP-exos was concentration dependent. 
      CONCLUSION: Intra-articular injection of PRP-exos and PRP can promote the repair 
      of articular cartilage defects, and the therapeutic effect of PRP-exos is better 
      than the same concentration of PRP. PRP-exos are expected to be an effective 
      treatment for cartilage repair and regeneration.
CI  - (c) 2023. The Author(s).
FAU - Zhao, Hangyu
AU  - Zhao H
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, No. 246, Xuefu Road, Nangang District, Harbin, 150001, Heilongjiang, 
      China.
AD  - Department of Orthopedics, Chengdu Seventh People's Hospital, No. 1188, 
      Shuangxing Avenue, Shuangliu District, Chengdu, 610044, Sichuan, China.
FAU - Zhao, Zihang
AU  - Zhao Z
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, No. 246, Xuefu Road, Nangang District, Harbin, 150001, Heilongjiang, 
      China.
FAU - Li, Dailuo
AU  - Li D
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, No. 246, Xuefu Road, Nangang District, Harbin, 150001, Heilongjiang, 
      China.
FAU - Wang, Xin
AU  - Wang X
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, No. 246, Xuefu Road, Nangang District, Harbin, 150001, Heilongjiang, 
      China.
FAU - Dai, Dehao
AU  - Dai D
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, No. 246, Xuefu Road, Nangang District, Harbin, 150001, Heilongjiang, 
      China.
FAU - Fu, Hailiang
AU  - Fu H
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, No. 246, Xuefu Road, Nangang District, Harbin, 150001, Heilongjiang, 
      China. 106860696@qq.com.
LA  - eng
PT  - Journal Article
DEP - 20230302
PL  - England
TA  - J Orthop Surg Res
JT  - Journal of orthopaedic surgery and research
JID - 101265112
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - 0 (Collagen Type II)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
SB  - IM
MH  - Animals
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Matrix Metalloproteinase 3
MH  - Collagen Type II
MH  - *Exosomes
MH  - Tissue Inhibitor of Metalloproteinase-1
MH  - Knee Joint
MH  - *Platelet-Rich Plasma
PMC - PMC9983202
OTO - NOTNLM
OT  - Cartilage defect
OT  - Cartilage repair
OT  - Exosome
OT  - Platelet-rich plasma
COIS- The authors declare that they have no conflict of interest.
EDAT- 2023/03/03 06:00
MHDA- 2023/03/07 06:00
PMCR- 2023/03/02
CRDT- 2023/03/02 23:35
PHST- 2022/09/03 00:00 [received]
PHST- 2023/02/03 00:00 [accepted]
PHST- 2023/03/02 23:35 [entrez]
PHST- 2023/03/03 06:00 [pubmed]
PHST- 2023/03/07 06:00 [medline]
PHST- 2023/03/02 00:00 [pmc-release]
AID - 10.1186/s13018-023-03576-0 [pii]
AID - 3576 [pii]
AID - 10.1186/s13018-023-03576-0 [doi]
PST - epublish
SO  - J Orthop Surg Res. 2023 Mar 2;18(1):160. doi: 10.1186/s13018-023-03576-0.