PMID- 36865477
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230304
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 9
IP  - 2
DP  - 2023 Feb
TI  - Forsythiaside A prevents zymosan A-induced cell migration in 
      neutrophil-differentiated HL-60 cells via PD-1/PD-L1 pathway.
PG  - e13490
LID - 10.1016/j.heliyon.2023.e13490 [doi]
LID - e13490
AB  - Neutrophils, which account for more than 80% of leukocyte, play an important role 
      in resolution of inflammation. Immune checkpoint molecules could be potential 
      biomarkers in immunosuppression. Forsythiaside A (FTA), a main constituent of 
      Forsythia suspensa (Thunb.) Vahl, provides a very significant anti-inflammatory 
      activity. Here we defined the immunological mechanisms of FTA by taking 
      programmed cell death-1 (PD-1)/programmed cell death-Ligand 1 (PD-L1) pathway 
      into consideration. FTA could inhibited cell migration in HL-60-derived 
      neutrophils in vitro, and this action appeared to be mediated via PD-1/PD-L1 
      depended JNK and p38 MAPK pathways. In vivo, FTA prevented PD-L1(+) neutrophils 
      infiltration and reduced the levels of tumor necrosis factor alpha (TNF-alpha), 
      interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1) and 
      interferon-gamma (IFN-gamma) after zymosan A-induced peritonitis. PD-1/PD-L1 
      inhibitor could abolish the suppression of FTA. The expression of inflammatory 
      cytokines and chemokines were positively correlated with PD-L1. Molecular docking 
      showed that FTA could bind to PD-L1. Taken together, FTA might prevent 
      neutrophils infiltration to exert inflammation resolution through PD-1/PD-L1 
      pathway.
CI  - (c) 2023 The Authors.
FAU - Zhang, Xinyu
AU  - Zhang X
AD  - Experiment Center for Teaching and Learning, Shanghai University of Traditional 
      Chinese Medicine, Shanghai, China.
FAU - Li, Aiyun
AU  - Li A
AD  - School of Pharmacy, Shanghai University of Traditional Chinese Medicine, 
      Shanghai, China.
FAU - Xu, Yue
AU  - Xu Y
AD  - School of Pharmacy, Shanghai University of Traditional Chinese Medicine, 
      Shanghai, China.
FAU - Lan, Jinshuai
AU  - Lan J
AD  - Experiment Center for Teaching and Learning, Shanghai University of Traditional 
      Chinese Medicine, Shanghai, China.
FAU - Liu, Yun
AU  - Liu Y
AD  - Experiment Center for Teaching and Learning, Shanghai University of Traditional 
      Chinese Medicine, Shanghai, China.
FAU - Li, Ling
AU  - Li L
AD  - Experiment Center for Teaching and Learning, Shanghai University of Traditional 
      Chinese Medicine, Shanghai, China.
FAU - Kang, Ping
AU  - Kang P
AD  - School of Pharmacy, Shanghai University of Traditional Chinese Medicine, 
      Shanghai, China.
FAU - Zhang, Tong
AU  - Zhang T
AD  - Experiment Center for Teaching and Learning, Shanghai University of Traditional 
      Chinese Medicine, Shanghai, China.
LA  - eng
PT  - Journal Article
DEP - 20230208
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC9970906
OTO - NOTNLM
OT  - Forsythiaside A
OT  - Immunosuppression
OT  - Inflammation
OT  - Neutrophils
OT  - PD-L1
COIS- The authors declare no competing interests.
EDAT- 2023/03/04 06:00
MHDA- 2023/03/04 06:01
PMCR- 2023/02/08
CRDT- 2023/03/03 02:31
PHST- 2022/06/10 00:00 [received]
PHST- 2023/01/09 00:00 [revised]
PHST- 2023/01/31 00:00 [accepted]
PHST- 2023/03/03 02:31 [entrez]
PHST- 2023/03/04 06:00 [pubmed]
PHST- 2023/03/04 06:01 [medline]
PHST- 2023/02/08 00:00 [pmc-release]
AID - S2405-8440(23)00697-7 [pii]
AID - e13490 [pii]
AID - 10.1016/j.heliyon.2023.e13490 [doi]
PST - epublish
SO  - Heliyon. 2023 Feb 8;9(2):e13490. doi: 10.1016/j.heliyon.2023.e13490. eCollection 
      2023 Feb.