PMID- 36866416
OWN - NLM
STAT- MEDLINE
DCOM- 20231114
LR  - 20231122
IS  - 1932-8494 (Electronic)
IS  - 1932-8486 (Linking)
VI  - 306
IP  - 12
DP  - 2023 Dec
TI  - Effects of electroacupuncture on glial scar generation in SCI model rats.
PG  - 3156-3168
LID - 10.1002/ar.25132 [doi]
AB  - Spinal cord injury (SCI) is a commonly occurring and severe form of central 
      nervous system (CNS) injury. Previous studies have demonstrated that 
      electroacupuncture (EA) therapy promotes recovery from SCI. In this study, we 
      observed changes in the glial scars of rats with SCI to gain insight into how EA 
      therapy positively influences locomotor function. The experimental rats were 
      randomly divided into three groups: the sham group, the SCI group and the 
      SCI + EA group. Rats in the SCI + EA group received a 28-day treatment course 
      using the Dazhui (GV14) acupoint and the Mingmen (GV4) acupoint for 20 min/day. 
      The Basso-Beattie-Bresnahan (BBB) score was used to estimate the neural function 
      of rats in all groups. We found that before sacrifice on Day 28, the BBB score 
      was significantly improved in the SCI + EA group, which was higher than that 
      observed in the SCI group. Hematoxylin-eosin staining revealed morphological 
      improvements in spinal cord tissues of the rats in the EA + SCI group with 
      reduced glial scars and cavities. Based on immunofluorescence staining, reactive 
      astrocytes overpopulated both the SCI and SCI + EA groups following SCI. 
      Moreover, improved generation of reactive astrocytes at lesions was observed in 
      the SCI + EA group compared with the SCI group. After treatment, EA inhibited 
      glial scar generation. EA effectively downregulated fibrillary acidic protein 
      (GFAP) and vimentin protein and mRNA expression levels, according to the results 
      from Western blot assays and reverse transcription-polymerase chain reaction 
      (RT-PCR). We hypothesized that these findings described might represent the 
      mechanism underlying EA inhibition of glial scar generation, morphological 
      improvements in tissues and promotion of neural recovery from SCI in rats.
CI  - (c) 2023 American Association for Anatomy.
FAU - Hu, Yu
AU  - Hu Y
AUID- ORCID: 0000-0003-3770-0791
AD  - School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing, People's Republic of China.
FAU - Zhao, Haobin
AU  - Zhao H
AUID- ORCID: 0000-0001-8976-7520
AD  - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, 
      Beijing, People's Republic of China.
FAU - Shi, Suhua
AU  - Shi S
AUID- ORCID: 0000-0002-1787-8878
AD  - Department of Rehabilitation, The Third Affiliated Hospital of Beijing University 
      of Chinese Medicine, Beijing, People's Republic of China.
FAU - Zhao, Yali
AU  - Zhao Y
AD  - School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing, People's Republic of China.
FAU - Gao, Xiaoming
AU  - Gao X
AD  - School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing, People's Republic of China.
FAU - Sun, Jingwen
AU  - Sun J
AD  - School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing, People's Republic of China.
FAU - Li, Zhigang
AU  - Li Z
AD  - School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese 
      Medicine, Beijing, People's Republic of China.
FAU - Yao, Haijiang
AU  - Yao H
AD  - Treatment Center of Traditional Chinese Medicine, Beijing Bo'ai Hospital, China 
      Rehabilitation Research Center, School of Rehabilitation, Capital Medical 
      University, Beijing, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230302
PL  - United States
TA  - Anat Rec (Hoboken)
JT  - Anatomical record (Hoboken, N.J. : 2007)
JID - 101292775
SB  - IM
MH  - Rats
MH  - Animals
MH  - Rats, Sprague-Dawley
MH  - Gliosis/pathology
MH  - *Electroacupuncture
MH  - *Spinal Cord Injuries/pathology
MH  - Spinal Cord/pathology
MH  - Cicatrix/therapy/pathology
OTO - NOTNLM
OT  - electroacupuncture therapy
OT  - glial scar
OT  - locomotive function
OT  - spinal cord injury
OT  - traditional Chinese medicine therapy
OT  - 中医传统疗法
OT  - 电针疗法
OT  - 胶质瘢痕
OT  - 脊髓损伤
OT  - 运动功能
EDAT- 2023/03/04 06:00
MHDA- 2023/11/14 06:43
CRDT- 2023/03/03 02:46
PHST- 2022/11/04 00:00 [revised]
PHST- 2022/07/04 00:00 [received]
PHST- 2022/11/15 00:00 [accepted]
PHST- 2023/11/14 06:43 [medline]
PHST- 2023/03/04 06:00 [pubmed]
PHST- 2023/03/03 02:46 [entrez]
AID - 10.1002/ar.25132 [doi]
PST - ppublish
SO  - Anat Rec (Hoboken). 2023 Dec;306(12):3156-3168. doi: 10.1002/ar.25132. Epub 2023 
      Mar 2.