PMID- 36866688
OWN - NLM
STAT- MEDLINE
DCOM- 20230322
LR  - 20230322
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 14
IP  - 6
DP  - 2023 Mar 20
TI  - In vivo administration of gut bacterial consortia replicates urolithin 
      metabotypes A and B in a non-urolithin-producing rat model.
PG  - 2657-2667
LID - 10.1039/d2fo03957e [doi]
AB  - Urolithin (Uro) production capacity and, consequently, at least partly, the 
      health effects attributed to ellagitannin and ellagic acid consumption vary among 
      individuals. The reason is that not all individuals have the gut bacterial 
      ecology needed to produce the different Uro metabolites. Three human urolithin 
      metabotypes (UM-A, UM-B, and UM-0) based on dissimilar Uro production profiles 
      have been described in populations worldwide. Recently, the gut bacterial 
      consortia involved in ellagic acid metabolism to yield the urolithin-producing 
      metabotypes (UM-A and UM-B) in vitro have been identified. However, the ability 
      of these bacterial consortia to customize urolithin production to mimic UM-A and 
      UM-B in vivo is still unknown. In the present study, two bacterial consortia were 
      assessed for their capacity to colonize the intestine of rats and convert UM-0 
      (Uro non-producers) animals into Uro-producers that mimic UM-A and UM-B, 
      respectively. Two consortia of Uro-producing bacteria were orally administered to 
      non-urolithin-producing Wistar rats for 4 weeks. Uro-producing bacterial strains 
      effectively colonized the rats' gut, and the ability to produce Uros was also 
      effectively transferred. Bacterial strains were well tolerated. No changes in 
      other gut bacteria, except Streptococcus reduction, or adverse effects on 
      haematological and biochemical parameters were observed. Besides, two novel qPCR 
      procedures were designed and successfully optimized to detect and quantify 
      Ellagibacter and Enterocloster genera in faecal samples. These results suggest 
      that the bacterial consortia are safe and could be potential probiotics for human 
      trials, which is especially relevant for UM-0 individuals, who cannot produce 
      bioactive Uros.
FAU - Iglesias-Aguirre, Carlos E
AU  - Iglesias-Aguirre CE
AUID- ORCID: 0000-0001-7164-7934
AD  - Laboratory of Food & Health, Research Group on Quality, Safety and Bioactivity of 
      Plant Foods, CEBAS-CSIC, Campus de Espinardo, Murcia 30100, Spain. 
      mvselma@cebas.csic.es.
FAU - Gonzalez-Sarrias, Antonio
AU  - Gonzalez-Sarrias A
AUID- ORCID: 0000-0002-3407-0678
AD  - Laboratory of Food & Health, Research Group on Quality, Safety and Bioactivity of 
      Plant Foods, CEBAS-CSIC, Campus de Espinardo, Murcia 30100, Spain. 
      mvselma@cebas.csic.es.
FAU - Cortes-Martin, Adrian
AU  - Cortes-Martin A
AUID- ORCID: 0000-0001-5542-4984
AD  - Laboratory of Food & Health, Research Group on Quality, Safety and Bioactivity of 
      Plant Foods, CEBAS-CSIC, Campus de Espinardo, Murcia 30100, Spain. 
      mvselma@cebas.csic.es.
AD  - APC Microbiome Ireland & School of Microbiology, University College Cork, T12 
      YT20 Cork, Ireland.
FAU - Romo-Vaquero, Maria
AU  - Romo-Vaquero M
AUID- ORCID: 0000-0002-0084-6060
AD  - Laboratory of Food & Health, Research Group on Quality, Safety and Bioactivity of 
      Plant Foods, CEBAS-CSIC, Campus de Espinardo, Murcia 30100, Spain. 
      mvselma@cebas.csic.es.
FAU - Osuna-Galisteo, Leire
AU  - Osuna-Galisteo L
AUID- ORCID: 0000-0002-0059-0181
AD  - Laboratory of Food & Health, Research Group on Quality, Safety and Bioactivity of 
      Plant Foods, CEBAS-CSIC, Campus de Espinardo, Murcia 30100, Spain. 
      mvselma@cebas.csic.es.
FAU - Ceron, Jose Joaquin
AU  - Ceron JJ
AD  - Interdisciplinary Laboratory of Clinical Analysis, Interlab-UMU, Regional Campus 
      of International Excellence Campus Mare Nostrum, University of Murcia, 30100 
      Espinardo, Murcia, Spain.
FAU - Espin, Juan Carlos
AU  - Espin JC
AUID- ORCID: 0000-0002-1068-8692
AD  - Laboratory of Food & Health, Research Group on Quality, Safety and Bioactivity of 
      Plant Foods, CEBAS-CSIC, Campus de Espinardo, Murcia 30100, Spain. 
      mvselma@cebas.csic.es.
FAU - Selma, Maria Victoria
AU  - Selma MV
AUID- ORCID: 0000-0002-6780-974X
AD  - Laboratory of Food & Health, Research Group on Quality, Safety and Bioactivity of 
      Plant Foods, CEBAS-CSIC, Campus de Espinardo, Murcia 30100, Spain. 
      mvselma@cebas.csic.es.
LA  - eng
PT  - Journal Article
DEP - 20230320
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
RN  - 19YRN3ZS9P (Ellagic Acid)
RN  - 0 (Coumarins)
RN  - 0 (Hydrolyzable Tannins)
SB  - IM
MH  - Humans
MH  - Animals
MH  - Rats
MH  - *Gastrointestinal Microbiome
MH  - Ellagic Acid/metabolism
MH  - Rats, Wistar
MH  - Feces/microbiology
MH  - Bacteria/genetics/metabolism
MH  - Coumarins/metabolism
MH  - Hydrolyzable Tannins/metabolism
EDAT- 2023/03/04 06:00
MHDA- 2023/03/23 06:00
CRDT- 2023/03/03 05:12
PHST- 2023/03/04 06:00 [pubmed]
PHST- 2023/03/23 06:00 [medline]
PHST- 2023/03/03 05:12 [entrez]
AID - 10.1039/d2fo03957e [doi]
PST - epublish
SO  - Food Funct. 2023 Mar 20;14(6):2657-2667. doi: 10.1039/d2fo03957e.