PMID- 36870027
OWN - NLM
STAT- MEDLINE
DCOM- 20231115
LR  - 20231122
IS  - 1559-0291 (Electronic)
IS  - 0273-2289 (Linking)
VI  - 195
IP  - 11
DP  - 2023 Nov
TI  - Integrated Gut Microbiota and Urine Metabolite Analyses of T2DM with NAFLD Rat 
      Model.
PG  - 6478-6494
LID - 10.1007/s12010-023-04419-8 [doi]
AB  - Globally 80% type 2 diabetes mellitus (T2DM) patients suffer nonalcoholic fatty 
      liver disease (NAFLD). The interplay of gut microbiota and endogenous metabolic 
      networks has not yet been reported in the setting of T2DM with NAFLD. As such, 
      this study utilized 16S rRNA gene sequencing to assess the changes in intestinal 
      flora and nuclear magnetic resonance spectroscopy ((1)H NMR) to identify 
      potential metabolites in a T2DM with NAFLD rat model. Spearman correlation 
      analysis was performed to explore the relationship between gut microbiota and 
      metabolites. Results revealed that among T2DM with NAFLD rats, diversity indexes 
      of intestinal microbiota were distinctly decreased while levels of 18 bacterial 
      genera within the intestinal tract were significantly altered. In addition, 
      levels of eight metabolites mainly involved in the synthesis and degradation of 
      ketone bodies, the TCA cycle, and butanoate metabolism were altered. Correlation 
      analysis revealed that gut bacteria such as Blautia, Ruminococcus torques group, 
      Allobaculum, and Lachnoclostridium strongly associate with 3-hydroxybutyrate, 
      acetone, acetoacetate, 2-oxoglutarate, citrate, creatinine, hippurate, and 
      allantoin. Our findings can provide a basis for future development of targeted 
      treatments.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Qin, Jinghua
AU  - Qin J
AD  - Pharmaceutical College, Guangxi Medical University, Nanning, 530021, China.
FAU - Ling, Xue
AU  - Ling X
AD  - Pharmaceutical College, Guangxi Medical University, Nanning, 530021, China.
FAU - Wang, Qianyi
AU  - Wang Q
AD  - Pharmaceutical College, Guangxi Medical University, Nanning, 530021, China.
FAU - Huang, Zheng
AU  - Huang Z
AD  - Pharmaceutical College, Guangxi Medical University, Nanning, 530021, China.
FAU - Guo, Bingjian
AU  - Guo B
AD  - Pharmaceutical College, Guangxi Medical University, Nanning, 530021, China.
FAU - Zhang, Chi
AU  - Zhang C
AD  - Pharmaceutical College, Guangxi Medical University, Nanning, 530021, China.
FAU - Meng, Mingwei
AU  - Meng M
AD  - Pharmaceutical College, Guangxi Medical University, Nanning, 530021, China.
FAU - Feng, Shisui
AU  - Feng S
AD  - Pharmaceutical College, Guangxi Medical University, Nanning, 530021, China.
FAU - Guo, Yue
AU  - Guo Y
AD  - Pharmaceutical College, Guangxi Medical University, Nanning, 530021, China.
AD  - Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, Guangxi 
      Institute of Traditional Medical and Pharmaceutical Sciences, Nanning, 530022, 
      People's Republic of China.
FAU - Zheng, Hua
AU  - Zheng H
AD  - Life Sciences Institute, Guangxi Medical University, Nanning, 530021, China. 
      215831305@qq.com.
FAU - Liang, Yonghong
AU  - Liang Y
AD  - Pharmaceutical College, Guangxi Medical University, Nanning, 530021, China. 
      lyhpharmacy@126.com.
FAU - Su, Zhiheng
AU  - Su Z
AUID- ORCID: 0000-0002-3908-3208
AD  - Pharmaceutical College, Guangxi Medical University, Nanning, 530021, China. 
      suzhiheng915@126.com.
AD  - Guangxi Key Laboratory of Bioactive Molecules Research and Evaluation, Nanning, 
      530021, China. suzhiheng915@126.com.
AD  - Guangxi Engineering Research Center for Beibu Gulf Marine Biomedicine Precision 
      Development and High-Value Utilization, Nanning, 530021, China. 
      suzhiheng915@126.com.
AD  - Guangxi Health Commission Key Laboratory of Basic Research On Antigeriatric 
      Drugs, Nanning, 530021, China. suzhiheng915@126.com.
LA  - eng
GR  - 81660697/Natural Science Foundation of China/
GR  - 81603533/Natural Science Foundation of China/
GR  - KY2016LX04/Program for Teacher's Promotion of Guangxi Higher Education 
      Institutions/
GR  - GXMUYSF13/the Youth Science Foundation of Guangxi Medical University/
GR  - GXMUYSF18/the Youth Science Foundation of Guangxi Medical University/
GR  - GXFCDP-PS-2018/Guangxi First-class Discipline Project for Pharmaceutical 
      Sciences/
GR  - 202002/Open Project of Guangxi Key Laboratory of Regenerative Medicine/
PT  - Journal Article
DEP - 20230304
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Humans
MH  - Rats
MH  - Animals
MH  - *Gastrointestinal Microbiome/genetics
MH  - *Diabetes Mellitus, Type 2/metabolism
MH  - *Non-alcoholic Fatty Liver Disease
MH  - RNA, Ribosomal, 16S/genetics
MH  - Magnetic Resonance Spectroscopy
OTO - NOTNLM
OT  - 16S rRNA gene sequencing
OT  - Gut microbiota
OT  - Metabolites
OT  - Metabolomics
OT  - T2DM with NAFLD
EDAT- 2023/03/05 06:00
MHDA- 2023/11/15 06:43
CRDT- 2023/03/04 11:19
PHST- 2023/02/17 00:00 [accepted]
PHST- 2023/11/15 06:43 [medline]
PHST- 2023/03/05 06:00 [pubmed]
PHST- 2023/03/04 11:19 [entrez]
AID - 10.1007/s12010-023-04419-8 [pii]
AID - 10.1007/s12010-023-04419-8 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2023 Nov;195(11):6478-6494. doi: 
      10.1007/s12010-023-04419-8. Epub 2023 Mar 4.