PMID- 36871346
OWN - NLM
STAT- MEDLINE
DCOM- 20230314
LR  - 20230314
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1219
DP  - 2023 Mar 15
TI  - Preparation of magnetic poly(ethylene glycol 
      dimethacrylate-N-Methacryloyl-(L)-glutamic acid) particles for thrombin 
      purification.
PG  - 123653
LID - S1570-0232(23)00063-6 [pii]
LID - 10.1016/j.jchromb.2023.123653 [doi]
AB  - In this study, magnetic poly(ethylene glycol 
      dimethacrylate-N-methacryloyl-(L)-glutamic acid) (mPEGDMA-MAGA) particles were 
      prepared by the dispersion polymerization in order to purify thrombin 
      effectively. mPEGDMA-MAGA particles were synthesized by adding different ratios 
      of magnetite (Fe(3)O(4)) to the medium in addition to the monomer phases EGDMA 
      and MAGA. The characterization studies of mPEGDMA-MAGA particles were used by 
      fourier transform infrared spectroscopy, zeta size measurement, scanning electron 
      microscopy and electron spin resonance. mPEGDMA-MAGA particles were used in 
      thrombin adsorption studies from aqueous thrombin solutions in both batch and 
      magnetically stabilized fluidized bed (MSFB) system. Maximum adsorption capacity 
      in pH 7.4 phosphate buffer solution is 964 IU/g polymer and 134 IU/g polymer in 
      MSFB system and batch system, respectively. The developed magnetic affinity 
      particles enabled the separation of thrombin from different patient serum samples 
      in one step. It has also been observed that magnetic particles can be used 
      repeatedly without significant reduction in adsorption capacity.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Cimen, Duygu
AU  - Cimen D
AD  - Hacettepe University, Department of Chemistry, Ankara, Turkey.
FAU - Bereli, Nilay
AU  - Bereli N
AD  - Hacettepe University, Department of Chemistry, Ankara, Turkey.
FAU - Gunaydin, Serdar
AU  - Gunaydin S
AD  - University of Health Sciences, Department of Cardiovascular Surgery, Ankara City 
      Hospital, Ankara, Turkey.
FAU - Denizli, Adil
AU  - Denizli A
AD  - Hacettepe University, Department of Chemistry, Ankara, Turkey. Electronic 
      address: denizli@hacettepe.edu.tr.
LA  - eng
PT  - Journal Article
DEP - 20230228
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 7BK5G69305 (ethylene dimethacrylate)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - EC 3.4.21.5 (Thrombin)
RN  - 0 (Methacrylates)
RN  - 0 (poly(ethylene glycol)-dimethacrylate)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
SB  - IM
MH  - Humans
MH  - *Glutamic Acid
MH  - *Thrombin
MH  - Hydrogen-Ion Concentration
MH  - Methacrylates/chemistry
MH  - Polyethylene Glycols
MH  - Adsorption
MH  - Magnetic Phenomena
OTO - NOTNLM
OT  - Magnetically stabilized fluidized bed (MSFB) system
OT  - Purification
OT  - Thrombin
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/03/06 06:00
MHDA- 2023/03/15 06:00
CRDT- 2023/03/05 18:06
PHST- 2023/01/05 00:00 [received]
PHST- 2023/02/21 00:00 [revised]
PHST- 2023/02/23 00:00 [accepted]
PHST- 2023/03/06 06:00 [pubmed]
PHST- 2023/03/15 06:00 [medline]
PHST- 2023/03/05 18:06 [entrez]
AID - S1570-0232(23)00063-6 [pii]
AID - 10.1016/j.jchromb.2023.123653 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2023 Mar 15;1219:123653. doi: 
      10.1016/j.jchromb.2023.123653. Epub 2023 Feb 28.