PMID- 36873027
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230307
IS  - 2470-1343 (Electronic)
IS  - 2470-1343 (Linking)
VI  - 8
IP  - 8
DP  - 2023 Feb 28
TI  - High Production of gamma-Aminobutyric Acid by Activating the xyl Operon of 
      Lactobacillus brevis.
PG  - 8101-8109
LID - 10.1021/acsomega.2c08272 [doi]
AB  - gamma-Aminobutyric acid (GABA) is an inhibitory neurotransmitter with important 
      physiological functions such as sleep assistance and anti-depression. In this 
      study, we developed a fermentation process for the high-efficiency production of 
      GABA by Lactobacillus brevis (Lb. brevis) CE701. First, xylose was found as the 
      optimal carbon source that could improve the GABA production and OD(600) in shake 
      flasks to 40.35 g/L and 8.64, respectively, which were 1.78-fold and 1.67-fold of 
      the glucose. Subsequently, the analysis of the carbon source metabolic pathway 
      indicated that xylose activated the expression of the xyl operon, and xylose 
      metabolism produced more ATP and organic acids than glucose, which significantly 
      promoted the growth and GABA production of Lb. brevis CE701. Then, an efficient 
      GABA fermentation process was developed by optimizing the medium components using 
      response surface methodology. Finally, the production of GABA reached 176.04 g/L 
      in a 5 L fermenter, which was 336% higher than that in a shake flask. This work 
      enables the efficient synthesis of GABA using xylose, which will provide guidance 
      for the industrial production of GABA.
CI  - (c) 2023 The Authors. Published by American Chemical Society.
FAU - Cha, Xingchang
AU  - Cha X
AUID- ORCID: 0000-0003-3104-3620
AD  - Chemical Engineering Research Center, School of Chemical Engineering and 
      Technology, Tianjin University, Tianjin 300072, P. R. China.
FAU - Ding, Juanjuan
AU  - Ding J
AD  - Chemical Engineering Research Center, School of Chemical Engineering and 
      Technology, Tianjin University, Tianjin 300072, P. R. China.
FAU - Ba, Wenyan
AU  - Ba W
AD  - Chemical Engineering Research Center, School of Chemical Engineering and 
      Technology, Tianjin University, Tianjin 300072, P. R. China.
FAU - You, Shengping
AU  - You S
AUID- ORCID: 0000-0003-3546-1555
AD  - Chemical Engineering Research Center, School of Chemical Engineering and 
      Technology, Tianjin University, Tianjin 300072, P. R. China.
AD  - Tianjin Key Laboratory of Membrane Science and Desalination Technology, Tianjin 
      University, Tianjin 300072, P. R. China.
FAU - Qi, Wei
AU  - Qi W
AUID- ORCID: 0000-0002-7378-1392
AD  - Chemical Engineering Research Center, School of Chemical Engineering and 
      Technology, Tianjin University, Tianjin 300072, P. R. China.
AD  - State Key Laboratory of Chemical Engineering, Tianjin University, Tianjin 300072, 
      P. R. China.
AD  - Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), 
      Tianjin 300072, P. R. China.
AD  - Tianjin Key Laboratory of Membrane Science and Desalination Technology, Tianjin 
      University, Tianjin 300072, P. R. China.
FAU - Su, Rongxin
AU  - Su R
AUID- ORCID: 0000-0001-9778-9113
AD  - Chemical Engineering Research Center, School of Chemical Engineering and 
      Technology, Tianjin University, Tianjin 300072, P. R. China.
AD  - State Key Laboratory of Chemical Engineering, Tianjin University, Tianjin 300072, 
      P. R. China.
AD  - Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), 
      Tianjin 300072, P. R. China.
AD  - Tianjin Key Laboratory of Membrane Science and Desalination Technology, Tianjin 
      University, Tianjin 300072, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20230217
PL  - United States
TA  - ACS Omega
JT  - ACS omega
JID - 101691658
PMC - PMC9979331
COIS- The authors declare no competing financial interest.
EDAT- 2023/03/07 06:00
MHDA- 2023/03/07 06:01
PMCR- 2023/02/17
CRDT- 2023/03/06 03:36
PHST- 2022/12/30 00:00 [received]
PHST- 2023/02/09 00:00 [accepted]
PHST- 2023/03/06 03:36 [entrez]
PHST- 2023/03/07 06:00 [pubmed]
PHST- 2023/03/07 06:01 [medline]
PHST- 2023/02/17 00:00 [pmc-release]
AID - 10.1021/acsomega.2c08272 [doi]
PST - epublish
SO  - ACS Omega. 2023 Feb 17;8(8):8101-8109. doi: 10.1021/acsomega.2c08272. eCollection 
      2023 Feb 28.