PMID- 36873549
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230307
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 9
IP  - 3
DP  - 2023 Mar
TI  - Effect of alkynyloxy derivatives of lawsone as an antifungal spray for acrylic 
      denture base: An in vitro study.
PG  - e13919
LID - 10.1016/j.heliyon.2023.e13919 [doi]
LID - e13919
AB  - OBJECTIVE: The purpose of this study was to (i) synthesize and develop an 
      alkynyloxy derivative of lawsone as an antifungal spray and (ii) assess the 
      antifungal spray's effectiveness in reducing the viability of Candida albicans 
      (C. albicans) on polymethylmethacrylate (PMMA) specimens. METHODS: Lawsone methyl 
      ether (LME) and its derivative, 2-(prop-2-ynyloxy)naphthalene-1,4-dione (compound 
      1) were synthesized and characterized. The synthetic compounds were screened for 
      antimicrobial activities against C. albicans using the microtiter broth dilution 
      method to determine the minimum inhibitory concentration (MIC) and minimum 
      fungicidal concentration (MFC). Compound 1 was further formulated as an 
      antifungal spray in three concentrations (100, 200, and 400 mug/mL). C. albicans 
      biofilms were developed for 48 h on PMMA specimens. The efficacy of using an 
      antifungal spray for 1 and 3 min to remove biofilm was assessed using colony 
      counting and scanning electron microscopy (SEM). Chlorhexidine gluconate (CHX), 
      polident(R), and distilled water were used as positive and negative control 
      cleansing solutions, respectively. RESULTS: LME and compound 1 showed comparable 
      inhibition against C. albicans with a MIC of 25 mug/mL and MFC of 50 mug/mL. For 
      immediate treatment, C. albicans was not detected on PMMA specimens when expose 
      to 2% CHX and compound 1 (100, 200, and 400 mug/mL) antifungal spray for 3 min. 
      However, after recolonization, a small number of viable cells were observed in 
      denture soaked in compound 1 antifungal spray for 3 min group. Following 
      recolonization, polident(R) and distilled water had comparable viable cell counts 
      of C. albicans to the no treatment group. Scanning electron microscope (SEM) 
      images revealed that CHX, polident(R), and compound 1 caused cell damage in various 
      forms. CONCLUSION: Denture spray containing synthetic alkynyloxy derivative of 
      lawsone is a promising antifungal agent for C. albicans biofilm removal from the 
      PMMA surface.
CI  - (c) 2023 The Authors.
FAU - Lomlim, Luelak
AU  - Lomlim L
AD  - Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, 
      Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.
AD  - Phytomedicine and Pharmaceutical Biotechnology Excellent Center (PPBEC), Faculty 
      of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, 
      90112, Thailand.
FAU - Manuschai, Jutharat
AU  - Manuschai J
AD  - Department of Conservative Dentistry, Faculty of Dentistry, Prince of Songkla 
      University, Hat Yai, Songkhla, 90112, Thailand.
FAU - Ratti, Pichayaporn
AU  - Ratti P
AD  - Department of Dental Public Health, Sirindhorn College of Public Health Yala, 
      Muang Yala, Yala, 95000, Thailand.
FAU - Kara, Jiraporn
AU  - Kara J
AD  - Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, 
      Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.
AD  - Phytomedicine and Pharmaceutical Biotechnology Excellent Center (PPBEC), Faculty 
      of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, 
      90112, Thailand.
FAU - Sakunphueak, Athip
AU  - Sakunphueak A
AD  - Phytomedicine and Pharmaceutical Biotechnology Excellent Center (PPBEC), Faculty 
      of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, 
      90112, Thailand.
AD  - Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical 
      Sciences, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.
FAU - Panichayupakaranant, Pharkphoom
AU  - Panichayupakaranant P
AD  - Phytomedicine and Pharmaceutical Biotechnology Excellent Center (PPBEC), Faculty 
      of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, 
      90112, Thailand.
AD  - Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical 
      Sciences, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.
FAU - Naorungroj, Supawadee
AU  - Naorungroj S
AD  - Department of Conservative Dentistry, Faculty of Dentistry, Prince of Songkla 
      University, Hat Yai, Songkhla, 90112, Thailand.
LA  - eng
PT  - Journal Article
DEP - 20230220
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC9982616
OTO - NOTNLM
OT  - Antifungal activity
OT  - Biofilm removal
OT  - Candida species
OT  - Denture hygiene
OT  - Lawsone derivative
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2023/03/07 06:00
MHDA- 2023/03/07 06:01
PMCR- 2023/02/20
CRDT- 2023/03/06 03:42
PHST- 2022/07/23 00:00 [received]
PHST- 2023/02/04 00:00 [revised]
PHST- 2023/02/15 00:00 [accepted]
PHST- 2023/03/06 03:42 [entrez]
PHST- 2023/03/07 06:00 [pubmed]
PHST- 2023/03/07 06:01 [medline]
PHST- 2023/02/20 00:00 [pmc-release]
AID - S2405-8440(23)01126-X [pii]
AID - e13919 [pii]
AID - 10.1016/j.heliyon.2023.e13919 [doi]
PST - epublish
SO  - Heliyon. 2023 Feb 20;9(3):e13919. doi: 10.1016/j.heliyon.2023.e13919. eCollection 
      2023 Mar.