PMID- 36876004
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230307
IS  - 2297-1769 (Print)
IS  - 2297-1769 (Electronic)
IS  - 2297-1769 (Linking)
VI  - 10
DP  - 2023
TI  - Evaluating oral swab samples for PRRSV surveillance in weaning-age pigs under 
      field conditions.
PG  - 1072682
LID - 10.3389/fvets.2023.1072682 [doi]
LID - 1072682
AB  - INTRODUCTION: The use of serum and family oral fluids for porcine reproductive 
      and respiratory syndrome virus (PRRSV) surveillance in weaning-age pigs has been 
      previously characterized. Characterizing more sample types similarly offers 
      veterinarians and producers additional validated sample options for PRRSV 
      surveillance in this subpopulation of pigs. Oral swab sampling is relatively easy 
      and convenient; however, there is sparse information on how it compares to the 
      reference sample type for PRRSV surveillance under field conditions. Therefore, 
      this study's objective was to compare the PRRSV reverse-transcription real-time 
      polymerase chain reaction (RT-rtPCR) test outcomes of oral swabs (OS) and sera 
      samples obtained from weaning-age pig litters. METHOD: At an eligible breeding 
      herd, six hundred twenty-three weaning-age piglets from 51 litters were each 
      sampled for serum and OS and tested for PRRSV RNA by RT-rtPCR. RESULTS AND 
      DISCUSSION: PRRSV RT-rtPCR positivity rate was higher in serum samples (24 of 51 
      litters, 83 of 623 pigs, with a mean cycle threshold (Ct) value of 
      RT-rtPCR-positive samples per litter ranging from 18.9 to 32.0) compared to OS 
      samples (15 of 51 litters, 33 of 623 pigs, with a mean Ct of RT-rtPCR positive 
      samples per litter ranging from 28.2 to 36.9); this highlights the importance of 
      interpreting negative RT-rtPCR results from OS samples with caution. Every litter 
      with a positive PRRSV RT-rtPCR OS had at least one viremic piglet, highlighting 
      the authenticity of positive PRRSV RT-rtPCR tests using OS; in other words, there 
      was no evidence of environmental PRRSV RNA being detected in OS. Cohen's kappa 
      analysis (Ck = 0.638) indicated a substantial agreement between both sample types 
      for identifying the true PRRSV status of weaning-age pigs.
CI  - Copyright (c) 2023 Osemeke, VanKley, LeFevre, Peterson and Linhares.
FAU - Osemeke, Onyekachukwu Henry
AU  - Osemeke OH
AD  - Fieldepi, Iowa State University College of Veterinary Medicine, Ames, IA, United 
      States.
FAU - VanKley, Nathan
AU  - VanKley N
AD  - College of Veterinary Medicine, Michigan State University, Lansing, MI, United 
      States.
FAU - LeFevre, Claire
AU  - LeFevre C
AD  - Carthage Veterinary Service, Carthage, IL, United States.
FAU - Peterson, Christina
AU  - Peterson C
AD  - Fieldepi, Iowa State University College of Veterinary Medicine, Ames, IA, United 
      States.
FAU - Linhares, Daniel C L
AU  - Linhares DCL
AD  - Fieldepi, Iowa State University College of Veterinary Medicine, Ames, IA, United 
      States.
LA  - eng
PT  - Journal Article
DEP - 20230203
PL  - Switzerland
TA  - Front Vet Sci
JT  - Frontiers in veterinary science
JID - 101666658
PMC - PMC9976936
OTO - NOTNLM
OT  - PRRSV
OT  - sampling
OT  - serum
OT  - surveillance
OT  - swabs
OT  - weaning-age
COIS- CL was employed by Carthage Veterinary Services, Limited. The remaining authors 
      declare that the research was conducted in the absence of any commercial or 
      financial relationships that could be construed as a potential conflict of 
      interest.
EDAT- 2023/03/07 06:00
MHDA- 2023/03/07 06:01
PMCR- 2023/01/01
CRDT- 2023/03/06 04:18
PHST- 2022/10/17 00:00 [received]
PHST- 2023/01/16 00:00 [accepted]
PHST- 2023/03/06 04:18 [entrez]
PHST- 2023/03/07 06:00 [pubmed]
PHST- 2023/03/07 06:01 [medline]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 10.3389/fvets.2023.1072682 [doi]
PST - epublish
SO  - Front Vet Sci. 2023 Feb 3;10:1072682. doi: 10.3389/fvets.2023.1072682. 
      eCollection 2023.