PMID- 36876081 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20230403 IS - 1664-302X (Print) IS - 1664-302X (Electronic) IS - 1664-302X (Linking) VI - 14 DP - 2023 TI - Improvement of antioxidant activity and active ingredient of Dendrobium officinale via microbial fermentation. PG - 1061970 LID - 10.3389/fmicb.2023.1061970 [doi] LID - 1061970 AB - This study used brewer's yeast to ferment Dendrobium officinale and single-factor and orthogonal experiments were conducted to determine the optimal fermentation conditions. The antioxidant capacity of Dendrobium fermentation solution was also investigated by in vitro experiments, which showed that different concentrations of fermentation solution could effectively enhance the total antioxidant capacity of cells. The fermentation liquid was found to contain seven sugar compounds including glucose, galactose, rhamnose, arabinose, and xylose using gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography-quadrupole-time of flight mass spectrometry (HPLC-Q-TOF-MS), with the highest concentrations of glucose and galactose at 194.628 and 103.899 mug/ml, respectively. The external fermentation liquid also contained six flavonoids with apigenin glycosides as the main structure and four phenolic acids including gallic acid, protocatechuic acid, catechol, and sessile pentosidine B. CI - Copyright (c) 2023 Yu, Xie, Su, Dai, Deng, Gu, Liu, Yun, Xiang, Xiong, Yang, Ren and Li. FAU - Yu, Gen AU - Yu G AD - Key Specialty of Clinical Pharmacy, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China. AD - Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, China. FAU - Xie, QingFen AU - Xie Q AD - Key Specialty of Clinical Pharmacy, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China. FAU - Su, WenFeng AU - Su W AD - Key Specialty of Clinical Pharmacy, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China. FAU - Dai, Shuang AU - Dai S AD - Key Specialty of Clinical Pharmacy, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China. FAU - Deng, XinYi AU - Deng X AD - Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, China. FAU - Gu, QuLiang AU - Gu Q AD - Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, China. FAU - Liu, Shan AU - Liu S AD - Guangzhou Base Clean Cosmetics Manufacturer Co., Ltd., Guangzhou, China. FAU - Yun, JeonYun AU - Yun J AD - Guangzhou Base Clean Cosmetics Manufacturer Co., Ltd., Guangzhou, China. FAU - Xiang, WenHao AU - Xiang W AD - Guangzhou Base Clean Cosmetics Manufacturer Co., Ltd., Guangzhou, China. FAU - Xiong, Yang AU - Xiong Y AD - Guangzhou Base Clean Cosmetics Manufacturer Co., Ltd., Guangzhou, China. FAU - Yang, GuanDong AU - Yang G AD - CAS Testing Technical Services (Guangzhou) Co., Ltd., Guangzhou, China. FAU - Ren, Yifei AU - Ren Y AD - School of Life Sciences and Biopharmaceuticals, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China. AD - Guangzhou Huashuo Biotechnology Co., Ltd., Guangzhou, China. FAU - Li, He AU - Li H AD - School of Life Sciences and Biopharmaceuticals, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, China. LA - eng PT - Journal Article DEP - 20230208 PL - Switzerland TA - Front Microbiol JT - Frontiers in microbiology JID - 101548977 EIN - Front Microbiol. 2023 Mar 16;14:1179511. PMID: 37007470 PMC - PMC9979217 OTO - NOTNLM OT - Dendrobium officinale OT - GC-MS OT - antioxidant OT - condition optimization OT - fermentation COIS- SL, JY, WX, and YX were employed by Guangzhou Base Clean Cosmetics Manufacturer Co., Ltd., GY was employed by CAS Testing Technical Services (Guangzhou) Co., Ltd., YR was employed by Guangzhou Huashuo Biotechnology Co. Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2023/03/07 06:00 MHDA- 2023/03/07 06:01 PMCR- 2023/02/08 CRDT- 2023/03/06 04:19 PHST- 2022/10/06 00:00 [received] PHST- 2023/01/09 00:00 [accepted] PHST- 2023/03/06 04:19 [entrez] PHST- 2023/03/07 06:00 [pubmed] PHST- 2023/03/07 06:01 [medline] PHST- 2023/02/08 00:00 [pmc-release] AID - 10.3389/fmicb.2023.1061970 [doi] PST - epublish SO - Front Microbiol. 2023 Feb 8;14:1061970. doi: 10.3389/fmicb.2023.1061970. eCollection 2023.