PMID- 36879410
OWN - NLM
STAT- MEDLINE
DCOM- 20230529
LR  - 20230529
IS  - 1460-2180 (Electronic)
IS  - 0143-3334 (Linking)
VI  - 44
IP  - 2
DP  - 2023 May 26
TI  - Enhancer RNA IRS2e is essential for IRS2 expression and the oncogenic properties 
      in oral squamous cell carcinoma.
PG  - 119-128
LID - 10.1093/carcin/bgad010 [doi]
AB  - The crucial roles of enhancer RNAs (eRNAs) in the regulation of gene expression 
      in human diseases have drawn wider and wider attention in recent years. However, 
      the specific expression profile and function of eRNAs are still rarely discussed 
      in oral squamous cell carcinoma (OSCC), the most common subtype of head and neck 
      squamous cell carcinoma (HNSC). In this study, we aimed to investigate the 
      expression and function of an uncharacterized eRNA, insulin receptor substrate 2 
      enhancer RNA (IRS2e), in OSCC. We found that IRS2e was overexpressed in HNSC and 
      its overexpression was positively correlated with a poor prognosis. The 
      downregulation of IRS2e by short hairpin RNA significantly inhibited cell growth 
      and induced cellular apoptosis and cell-cycle arrest in OSCC cells. Furthermore, 
      the ablation of IRS2e inhibited tumor growth in vivo. Mechanically, IRS2e is 
      essential for the expression of insulin receptor substrate 2 (IRS2), an oncogene 
      nearby IRS2e in chromosome 13. Altogether, our study demonstrated that IRS2e is a 
      novel oncogenic eRNA required for oncogene IRS2 expression in OSCC.
CI  - (c) The Author(s) 2023. Published by Oxford University Press. All rights reserved. 
      For Permissions, please email: journals.permissions@oup.com.
FAU - Yan, Lingyan
AU  - Yan L
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School 
      and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.
FAU - Wang, Jiayun
AU  - Wang J
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School 
      and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.
FAU - Guo, Jihua
AU  - Guo J
AUID- ORCID: 0000-0002-6713-4439
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School 
      and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.
AD  - Department of Endodontics, School and Hospital of Stomatology, Wuhan University, 
      Wuhan, 430079, China.
FAU - Jia, Rong
AU  - Jia R
AUID- ORCID: 0000-0001-6960-6199
AD  - The State Key Laboratory Breeding Base of Basic Science of Stomatology 
      (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School 
      and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (IRS2 protein, human)
SB  - IM
MH  - Humans
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
MH  - *Carcinoma, Squamous Cell/pathology
MH  - *Mouth Neoplasms/pathology
MH  - Insulin Receptor Substrate Proteins/genetics/metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - RNA, Small Interfering
MH  - *Head and Neck Neoplasms/genetics
MH  - Gene Expression Regulation, Neoplastic
EDAT- 2023/03/08 06:00
MHDA- 2023/05/29 06:42
CRDT- 2023/03/07 00:13
PHST- 2022/11/15 00:00 [received]
PHST- 2023/02/18 00:00 [revised]
PHST- 2023/03/01 00:00 [accepted]
PHST- 2023/05/29 06:42 [medline]
PHST- 2023/03/08 06:00 [pubmed]
PHST- 2023/03/07 00:13 [entrez]
AID - 7069775 [pii]
AID - 10.1093/carcin/bgad010 [doi]
PST - ppublish
SO  - Carcinogenesis. 2023 May 26;44(2):119-128. doi: 10.1093/carcin/bgad010.