PMID- 36879492
OWN - NLM
STAT- MEDLINE
DCOM- 20230503
LR  - 20230508
IS  - 1544-2217 (Electronic)
IS  - 0300-9858 (Linking)
VI  - 60
IP  - 3
DP  - 2023 May
TI  - Pathogenesis of Kasokero virus in experimentally infected Egyptian rousette bats 
      (Rousettus aegyptiacus).
PG  - 324-335
LID - 10.1177/03009858231158076 [doi]
AB  - Egyptian rousette bats (ERBs; Rousettus aegyptiacus; family Pteropodidae) are 
      associated with a growing number of bunyaviruses of public health importance, 
      including Kasokero virus (KASV), which was first identified as a zoonosis in 
      Uganda in 1977. In this study, formalin-fixed paraffin-embedded tissues from a 
      previous experiment in which KASV infection was confirmed in 18 experimentally 
      infected ERBs were used for an in-depth analysis using histopathology, in situ 
      hybridization (ISH) for detection of viral RNA, immunohistochemistry (IHC) to 
      assess the mononuclear phagocyte system response, and quantitative digital image 
      analysis to investigate virus clearance from the liver and spleen within a 
      spatial context. Significant gross and histological lesions were limited to the 
      liver, where KASV-infected bats developed mild to moderate, acute viral 
      hepatitis, which was first observed at 3 days postinfection (DPI), peaked at 6 
      DPI, and was resolved by 20 DPI. A subset of bats had glycogen depletion (n = 10) 
      and hepatic necrosis (n = 3), rarely with intralesional bacteria (n = 1). Virus 
      replication was confirmed by ISH in the liver, spleen, lymph nodes, and tongue. 
      In the liver, KASV replicated in the cytoplasm of hepatocytes, to a lesser extent 
      in mononuclear phagocytes, and rarely in presumptive endothelial cells. Most KASV 
      RNA, as detected by ISH, was cleared from the spleen and liver by 6 DPI. It is 
      concluded that ERBs have effective mechanisms to respond to this virus, clearing 
      it without evidence of clinical disease.
FAU - Kirejczyk, Shannon G M
AU  - Kirejczyk SGM
AUID- ORCID: 0000-0002-5900-4239
AD  - University of Georgia College of Veterinary Medicine, Athens, GA.
AD  - US Centers for Disease Control and Prevention, Atlanta, GA.
FAU - Schuh, Amy J
AU  - Schuh AJ
AD  - US Centers for Disease Control and Prevention, Atlanta, GA.
AD  - US Public Health Service Commissioned Corps, Rockville, MD.
FAU - Zhang, Jian
AU  - Zhang J
AD  - University of Georgia College of Veterinary Medicine, Athens, GA.
FAU - Amman, Brian R
AU  - Amman BR
AD  - US Centers for Disease Control and Prevention, Atlanta, GA.
FAU - Guito, Jonathan C
AU  - Guito JC
AD  - US Centers for Disease Control and Prevention, Atlanta, GA.
FAU - Sealy, Tara K
AU  - Sealy TK
AD  - US Centers for Disease Control and Prevention, Atlanta, GA.
FAU - Graziano, James C
AU  - Graziano JC
AD  - US Centers for Disease Control and Prevention, Atlanta, GA.
FAU - Brown, Corrie C
AU  - Brown CC
AUID- ORCID: 0000-0002-0332-6159
AD  - University of Georgia College of Veterinary Medicine, Athens, GA.
FAU - Towner, Jonathan S
AU  - Towner JS
AD  - University of Georgia College of Veterinary Medicine, Athens, GA.
AD  - US Centers for Disease Control and Prevention, Atlanta, GA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20230306
PL  - United States
TA  - Vet Pathol
JT  - Veterinary pathology
JID - 0312020
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - *Chiroptera
MH  - Disease Reservoirs
MH  - Endothelial Cells
MH  - *Virus Diseases/veterinary
MH  - Liver/pathology
MH  - RNA, Viral
OTO - NOTNLM
OT  - Egyptian rousette
OT  - Kasokero virus
OT  - bats
OT  - bunyavirus
OT  - nairovirus
OT  - pathogenesis
OT  - viral hepatitis
OT  - zoonosis
EDAT- 2023/03/08 06:00
MHDA- 2023/05/03 06:42
CRDT- 2023/03/07 01:04
PHST- 2023/05/03 06:42 [medline]
PHST- 2023/03/08 06:00 [pubmed]
PHST- 2023/03/07 01:04 [entrez]
AID - 10.1177/03009858231158076 [doi]
PST - ppublish
SO  - Vet Pathol. 2023 May;60(3):324-335. doi: 10.1177/03009858231158076. Epub 2023 Mar 
      6.