PMID- 36880860
OWN - NLM
STAT- Publisher
LR - 20240216
IS - 0191-2917 (Print)
IS - 0191-2917 (Linking)
DP - 2023 Mar 7
TI - First Report of Anthracnose Caused by Colletotrichum camelliae on Amorphophallus
konjac in Hunan Province of China.
LID - 10.1094/PDIS-01-23-0150-PDN [doi]
AB - Amorphophallus konjac is a crop in the family Araceae, which is widely cultivated
in Hunan, Yunnan, and Guizhou in China. A. konjac flour is highly valuable
economically as a product for weight reduction. In June 2022, a new leaf disease
was discovered in an understory A. konjac plantation in Xupu County, Hunan
Province, China, where 2,000 hm(2) of A. konjac had been planted. Approximately
40% of the total cultivated area exhibited symptoms. The disease outbreaks
occurred during warm and wet weather (May to June). In the early stages of
infection, small brown spots appeared on the leaves and then gradually spread
into irregular lesions. There was a light yellow halo around the brown lesions.
In severe cases, the whole plant gradually turned yellow and died. Six
symptomatic leaf samples were collected from three different fields in Xupu
County to isolate the causal agent. They were rinsed with sterile water, and the
lesions were cut off. The lesions were rinsed in 3% hydrogen peroxide for 30 s
followed by a 90 s treatment in 75% alcohol. They were then rinsed five times in
sterile water, placed on water agar plates and incubated for 2-3 days at 28 degrees C.
After the mycelium had grown, they were transferred to potato dextrose agar (PDA)
plates and incubated for 3-5 days at 28 degrees C. In total, ten isolates were obtained,
and seven of them were Colletotrichum (isolation frequency 70%). Three
representative isolates (HY1, HY2, and HY3) were selected for further study. This
fungus grew as circular white colonies, which then became grey. The older
colonies looked like cotton and had dense aerial hyphae. The conidia were
cylindrical, lacked a septum, and were thin-walled. They measured 14.04 to 21.58
x 5.89 to 10.40 mum (n=100). To further confirm its fungal identity, the fungus
was amplified and sequenced using six genetic regions, including beta-tubulin
(TUB2), actin (ACT), the internal transcribed spacer (ITS), glyceraldehyde
3-phosphate dehydrogenase (GAPDH), calmodulin (CAL) and chitin synthase (CHS).
The universal primers BT2a/TUB2R, ACT512F/ACT783R, ITS4/ITS5, GDF/GDR, CL1C/CL2C,
CHS79F/CHS345R were used for amplification (Weir et al. 2012), sequenced by the
Sanger chain termination method, and submitted to GenBank (TUB2: OQ506549,
OQ506544, OP604480; ACT: OQ506551, OQ506546, OP604482; ITS: OQ457036, OQ457498,
OP458555; GAPDH: OQ506553, OQ506548, OP604484; CAL: OQ506552, OQ506547, OP604483;
CHS: OQ506550, OQ506545, OP604481). An analysis of a joint phylogenetic tree that
was constructed using the six genes showed that the three isolates clearly
clustered with Colletotrichum camelliae (syn. Glomerella cingulata f. sp.
camelliae) strain ICMP 10646 (GenBank: JX010437.1, JX009563.1, JX010225.1,
JX009993.1, JX009629.1, JX009892.1) and HUN1A4 (GenBank: KU252173.1, KU251646.1,
KU251565.1, KU252019.1, KU251838.1, KU251913.1). HY3 was used as a representative
strain for the pathogenicity test on the leaves of A. konjac from the whole
plant. PDA blocks that were 6 x 6 mm and had been cultured for 5 d were placed on
the leaf surface, and sterile PDA blocks were used as the control group. The
climate chamber was maintained at 28 degrees C at all times, and 90% relative humidity
was maintained. The pathogenic lesions appeared after 10 days of inoculation. The
pathogen that was re-isolated from the diseased tissues had the same
morphological characteristics as HY3. Thus, Koch's postulates were fulfilled. C.
camelliae has been shown to be the primary pathogenic fungus responsible for
anthracnose in tea (Ca. sinensis (L.) O. Kuntze) (Wang et al. 2016) and camellia
oleifera (Ca. oleifera Abel.) (Li et al. 2016). Anthracnose caused by
Colletotrichum gloeosporioides has been reported on A. konjac (Li. 2021).
However, to our knowledge, this is the first report in China and worldwide that
C. camelliae causes anthracnose on A. konjac. This research lays the foundation
for future research to control this disease.
FAU - Sun, Lei
AU - Sun L
AD - Huaihua University, 71205, No.180 Huaidong Road, Huaihua, China, 418000;
leisun248@126.com.
FAU - Wu, Si
AU - Wu S
AD - Huaihua, China; 2483104255@qq.com.
FAU - Lu, YeZi
AU - Lu Y
AD - HuaiHua, China; 2717366796@qq.com.
FAU - Wu, FuJie
AU - Wu F
AD - Huaihua, China; 2716954552@qq.com.
FAU - Chen, Xu
AU - Chen X
AD - Huaihua, China; chenxu0227@126.com.
LA - eng
PT - Journal Article
DEP - 20230307
PL - United States
TA - Plant Dis
JT - Plant disease
JID - 9882809
SB - IM
OTO - NOTNLM
OT - Amorphophallus konjac
OT - Colletotrichum camelliae
OT - Anthracnose
EDAT- 2023/03/08 06:00
MHDA- 2023/03/08 06:00
CRDT- 2023/03/07 09:43
PHST- 2023/03/07 09:43 [entrez]
PHST- 2023/03/08 06:00 [pubmed]
PHST- 2023/03/08 06:00 [medline]
AID - 10.1094/PDIS-01-23-0150-PDN [doi]
PST - aheadofprint
SO - Plant Dis. 2023 Mar 7. doi: 10.1094/PDIS-01-23-0150-PDN.