PMID- 36881561 OWN - NLM STAT- MEDLINE DCOM- 20230412 LR - 20240502 IS - 1522-1504 (Electronic) IS - 1040-0605 (Print) IS - 1040-0605 (Linking) VI - 324 IP - 5 DP - 2023 May 1 TI - Rat bronchoalveolar lavage proteome changes following e-cigarette aerosol exposures. PG - L571-L583 LID - 10.1152/ajplung.00016.2023 [doi] AB - E-cigarette liquids are complex mixtures of chemicals consisting of humectants, such as propylene glycol (PG) and vegetable glycerin (VG), with nicotine or flavorings added. Published literature emphasizes the toxicity of e-cigarette aerosols with flavorings whereas much less attention has been given to the biologic effects of humectants. The purpose of the current study was to provide a comprehensive view of the acute biologic effects of e-cigarette aerosols on rat bronchoalveolar lavage (BAL) using mass spectrometry-based global proteomics. Sprague-Dawley rats were exposed to e-cigarette aerosol for 3 h/day for three consecutive days. Groups included: PG/VG alone, PG/VG + 2.5% nicotine (N), or PG/VG + N + 3.3% vanillin (V). Right lung lobes were lavaged for BAL and supernatants prepared for proteomics. Extracellular BAL S100A9 concentrations and BAL cell staining for citrullinated histone H3 (citH3) were also performed. From global proteomics, approximately 2,100 proteins were identified from rat BAL. The greatest change in number of BAL proteins occurred with PG/VG exposures alone compared with controls with biological pathways enriched for acute phase responses, extracellular trap formation, and coagulation. Extracellular BAL S100A9 concentrations and the number of citH3 + BAL cells also increased significantly in PG/VG and PG/VG + 2.5% N. In contrast to PG/VG or PG/VG + N, the addition of vanillin to PG/VG + N increased BAL neutrophilia and downregulated lipid transport proteins. In summary, global proteomics support e-cigarette aerosol exposures to PG/VG alone as having a significant biologic effect on the lung independent of nicotine or flavoring with increased markers of extracellular trap formation. FAU - Day, Nicholas J AU - Day NJ AD - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, United States. FAU - Wang, Juan AU - Wang J AD - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, United States. FAU - Johnston, Carl J AU - Johnston CJ AD - Division of Pulmonology, Department of Pediatric Pulmonology, University of Rochester Medical Center, Rochester, New York, United States. AD - Department of Environmental Medicine, University of Rochester Medical Center, Rochester, New York, United States. FAU - Kim, So-Young AU - Kim SY AUID- ORCID: 0000-0001-8724-9770 AD - Division of Pulmonology, Department of Pediatric Pulmonology, University of Rochester Medical Center, Rochester, New York, United States. AD - Department of Environmental Medicine, University of Rochester Medical Center, Rochester, New York, United States. FAU - Olson, Heather M AU - Olson HM AUID- ORCID: 0000-0002-4501-9587 AD - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, United States. FAU - House, Emma L AU - House EL AD - Division of Pulmonology, Department of Pediatric Pulmonology, University of Rochester Medical Center, Rochester, New York, United States. AD - Department of Environmental Medicine, University of Rochester Medical Center, Rochester, New York, United States. FAU - Attah, Isaac Kwame AU - Attah IK AD - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, United States. FAU - Clair, Geremy C AU - Clair GC AUID- ORCID: 0000-0003-0478-8520 AD - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, United States. FAU - Qian, Wei-Jun AU - Qian WJ AUID- ORCID: 0000-0002-5393-2827 AD - Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, United States. FAU - McGraw, Matthew D AU - McGraw MD AUID- ORCID: 0000-0002-5646-3560 AD - Division of Pulmonology, Department of Pediatric Pulmonology, University of Rochester Medical Center, Rochester, New York, United States. AD - Department of Environmental Medicine, University of Rochester Medical Center, Rochester, New York, United States. LA - eng SI - figshare/10.6084/m9.figshare.22121615.v2 GR - L40 ES030909/ES/NIEHS NIH HHS/United States GR - P30 ES001247/ES/NIEHS NIH HHS/United States GR - R01 HL139335/HL/NHLBI NIH HHS/United States GR - T32 HL066988/HL/NHLBI NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20230307 PL - United States TA - Am J Physiol Lung Cell Mol Physiol JT - American journal of physiology. Lung cellular and molecular physiology JID - 100901229 RN - 6M3C89ZY6R (Nicotine) RN - CHI530446X (vanillin) RN - 0 (Proteome) RN - 0 (Hygroscopic Agents) RN - 6DC9Q167V3 (Propylene Glycol) RN - PDC6A3C0OX (Glycerol) RN - 0 (Aerosols) RN - 0 (Histones) RN - 0 (Flavoring Agents) RN - 0 (Biological Products) SB - IM MH - Rats MH - Animals MH - Nicotine MH - *Electronic Nicotine Delivery Systems MH - Proteome MH - Hygroscopic Agents MH - Rats, Sprague-Dawley MH - Propylene Glycol/pharmacology MH - Glycerol/pharmacology MH - Aerosols MH - Histones MH - Flavoring Agents MH - Bronchoalveolar Lavage MH - *Biological Products PMC - PMC10085554 OTO - NOTNLM OT - NETosis OT - PG/VG OT - e-cigarette OT - nicotine OT - vanillin COIS- No conflicts of interest, financial or otherwise, are declared by the authors. EDAT- 2023/03/08 06:00 MHDA- 2023/04/12 06:42 PMCR- 2024/05/01 CRDT- 2023/03/07 13:23 PHST- 2023/04/12 06:42 [medline] PHST- 2023/03/08 06:00 [pubmed] PHST- 2023/03/07 13:23 [entrez] PHST- 2024/05/01 00:00 [pmc-release] AID - L-00016-2023 [pii] AID - 10.1152/ajplung.00016.2023 [doi] PST - ppublish SO - Am J Physiol Lung Cell Mol Physiol. 2023 May 1;324(5):L571-L583. doi: 10.1152/ajplung.00016.2023. Epub 2023 Mar 7.