PMID- 36881963
OWN - NLM
STAT- MEDLINE
DCOM- 20230404
LR  - 20230404
IS  - 2772-9508 (Electronic)
IS  - 2772-9508 (Linking)
VI  - 148
DP  - 2023 May
TI  - Biogenic silver NPs alleviate LPS-induced neuroinflammation in a human fetal 
      brain-derived cell line: Molecular switch to the M2 phenotype, modulation of 
      TLR4/MyD88 and Nrf2/HO-1 signaling pathways, and molecular docking analysis.
PG  - 213363
LID - S2772-9508(23)00086-9 [pii]
LID - 10.1016/j.bioadv.2023.213363 [doi]
AB  - Silver nanoparticles (AgNPs) have inconsistent findings against inflammation. 
      Although a wealth of literature on the beneficial effects of green-synthesized 
      AgNPs has been published, a detailed mechanistic study of green AgNPs on the 
      protective effects against lipopolysaccharide (LPS)-induced neuroinflammation 
      using human microglial cells (HMC3) has not yet been reported. For the first 
      time, we studied the inhibitory effect of biogenic AgNPs on inflammation and 
      oxidative stress induced by LPS in HMC3 cells. X-ray photoelectron spectroscopy, 
      Fourier-transform infrared spectroscopy, and transmission electron microscopy 
      were used to characterize AgNPs produced from honeyberry. Co-treatment with AgNPs 
      significantly reduced mRNA expressions of inflammatory molecules such as 
      interleukin (IL)-6 and tumor necrosis factor-alpha, while increasing the expressions 
      of anti-inflammatory markers such as IL-10 and transforming growth factor 
      (TGF)-beta. HMC3 cells were also switched from M1 to M2, as shown by lower 
      expression of M1 markers such as cluster of differentiation (CD)80, CD86, and 
      CD68 and higher expression of M2 markers such as CD206, CD163, and triggering 
      receptors expressed on myeloid cells (TREM2). Furthermore, AgNPs inhibited 
      LPS-induced toll-like receptor (TLR)4 signaling, as evidenced by decreased 
      expression of myeloid differentiation factor 88 (MyD88) and TLR4. In addition, 
      AgNPs reduced the production of reactive oxygen species (ROS) and enhanced the 
      expression of nuclear factor-E2-related factor 2 (Nrf2) and heme oxygenase-1 
      (HO-1), while decreasing the expression of inducible nitric oxide synthase. The 
      docking score of the honeyberry phytoconstituents ranged from -14.93 to - 4.28 
      KJ/mol. In conclusion, biogenic AgNPs protect against neuroinflammation and 
      oxidative stress by targeting TLR4/MyD88 and Nrf2/HO-1 signaling pathways in a 
      LPS-induced in vitro model. Biogenic AgNPs could be utilized as potential 
      nanomedicine against LPS-induced inflammatory disorders.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Sharma, Anshul
AU  - Sharma A
AD  - College of BioNano Technology, Department of Food and Nutrition, Gachon 
      University, Gyeonggi-do 13120, Republic of Korea.
FAU - Sanjay
AU  - Sanjay
AD  - Department of Food Science and Biotechnology, Gachon University, Gyeonggi-do 
      13120, Republic of Korea.
FAU - Jaiswal, Varun
AU  - Jaiswal V
AD  - College of BioNano Technology, Department of Food and Nutrition, Gachon 
      University, Gyeonggi-do 13120, Republic of Korea.
FAU - Park, Miey
AU  - Park M
AD  - College of BioNano Technology, Department of Food and Nutrition, Gachon 
      University, Gyeonggi-do 13120, Republic of Korea; Institute for Aging and 
      Clinical Nutrition Research, Gachon University, Gyeonggi-do 13120, Republic of 
      Korea.
FAU - Lee, Hae-Jeung
AU  - Lee HJ
AD  - College of BioNano Technology, Department of Food and Nutrition, Gachon 
      University, Gyeonggi-do 13120, Republic of Korea; Institute for Aging and 
      Clinical Nutrition Research, Gachon University, Gyeonggi-do 13120, Republic of 
      Korea; Department of Health Sciences and Technology, GAIHST, Gachon University, 
      Incheon 21999, Republic of Korea. Electronic address: skysea@gachon.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20230303
PL  - Netherlands
TA  - Biomater Adv
JT  - Biomaterials advances
JID - 9918383886206676
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 3M4G523W1G (Silver)
RN  - 0 (NF-E2-Related Factor 2)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (TLR4 protein, human)
SB  - IM
MH  - Humans
MH  - *Lipopolysaccharides/toxicity
MH  - Myeloid Differentiation Factor 88/genetics/metabolism/pharmacology
MH  - Molecular Docking Simulation
MH  - Silver/pharmacology
MH  - NF-E2-Related Factor 2/genetics/metabolism/pharmacology
MH  - Neuroinflammatory Diseases
MH  - Heme Oxygenase-1/genetics/metabolism/pharmacology
MH  - Toll-Like Receptor 4/genetics/metabolism
MH  - *Metal Nanoparticles/therapeutic use
MH  - Signal Transduction
MH  - Phenotype
MH  - Cell Line
MH  - Inflammation/chemically induced/metabolism
MH  - Brain/metabolism
OTO - NOTNLM
OT  - Inflammation
OT  - Interleukin
OT  - Lipopolysaccharide
OT  - Microglia
OT  - Silver nanoparticles
OT  - Toll-like receptor
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/03/08 06:00
MHDA- 2023/04/04 06:42
CRDT- 2023/03/07 18:03
PHST- 2022/09/29 00:00 [received]
PHST- 2023/02/23 00:00 [revised]
PHST- 2023/02/25 00:00 [accepted]
PHST- 2023/04/04 06:42 [medline]
PHST- 2023/03/08 06:00 [pubmed]
PHST- 2023/03/07 18:03 [entrez]
AID - S2772-9508(23)00086-9 [pii]
AID - 10.1016/j.bioadv.2023.213363 [doi]
PST - ppublish
SO  - Biomater Adv. 2023 May;148:213363. doi: 10.1016/j.bioadv.2023.213363. Epub 2023 
      Mar 3.