PMID- 36882148
OWN - NLM
STAT- MEDLINE
DCOM- 20230321
LR  - 20230321
IS  - 1879-0712 (Electronic)
IS  - 0014-2999 (Linking)
VI  - 945
DP  - 2023 Apr 15
TI  - Influence of adrenalectomy on the gut microbiome and MDMA-induced hyperthermia.
PG  - 175643
LID - S0014-2999(23)00154-1 [pii]
LID - 10.1016/j.ejphar.2023.175643 [doi]
AB  - The increased use of the stimulant drug, 3,4-methylenedioxymethamphetamine 
      (MDMA), more commonly known as Ecstasy, Molly or X, has been linked to the 
      development of life-threatening hyperthermia in human and animal models. The 
      current study aimed to investigate the role of the gut-adrenal axis in 
      MDMA-induced hyperthermia by assessing the influence of the acute exogenous 
      supplementation with norepinephrine (NE) or corticosterone (CORT) to 
      adrenalectomized (ADX) rats following MDMA administration. MDMA (10 mg/kg, sc) 
      resulted in significant increase of body temperature in SHAM animals compared to 
      ADX animals at 30-, 60- and 90-min timepoints post-MDMA treatment. The attenuated 
      MDMA-mediated hyperthermic response seen in ADX animals was partially restored by 
      the exogenous administration of NE (3 mg/kg, ip) or CORT (3 mg/kg, ip) 30 min 
      after MDMA treatment. Additionally, 16 S rRNA analysis revealed distinct changes 
      in the gut microbiome composition and diversity notable by the higher abundance 
      of minor phyla Actinobacteria, Verrucomicrobia and Proteobacteria in ADX rats 
      compared to control and SHAM rats. Furthermore, MDMA administration resulted in 
      marked changes in the dominant phyla Firmicutes and Bacteroidetes and minor phyla 
      Actinobacteria, Verrucomicrobia and Proteobacteria in ADX animals. The most 
      notable changes in the gut microbiome upon CORT treatment were reported with 
      increase in Bacteroidetes and decrease in Firmicutes phyla whereas NE treatment 
      resulted in increase in Firmicutes and decrease in Bacteroidetes and 
      Proteobacteria post treatment. These results suggest a correlation between the 
      sympathoadrenal axis, gut microbiome structure and diversity and MDMA-mediated 
      hyperthermia.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Aburahma, Amal
AU  - Aburahma A
AD  - The Ohio Attorney General's Center for the Future of Forensic Science, USA.
FAU - Rana, Srishti
AU  - Rana S
AD  - The Department of Biological Sciences, Bowling Green State University, Bowling 
      Green, OH, 43403, USA.
FAU - Larsen, Ray
AU  - Larsen R
AD  - The Department of Biological Sciences, Bowling Green State University, Bowling 
      Green, OH, 43403, USA.
FAU - Ward, Christopher S
AU  - Ward CS
AD  - The Department of Biological Sciences, Bowling Green State University, Bowling 
      Green, OH, 43403, USA.
FAU - Sprague, Jon E
AU  - Sprague JE
AD  - The Ohio Attorney General's Center for the Future of Forensic Science, USA. 
      Electronic address: jesprag@bgsu.edu.
LA  - eng
PT  - Journal Article
DEP - 20230305
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - W980KJ009P (Corticosterone)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Humans
MH  - Rats
MH  - Animals
MH  - *N-Methyl-3,4-methylenedioxyamphetamine/pharmacology
MH  - Adrenalectomy
MH  - *Gastrointestinal Microbiome
MH  - Body Temperature
MH  - *Hyperthermia, Induced
MH  - Corticosterone/pharmacology
MH  - Norepinephrine
EDAT- 2023/03/08 06:00
MHDA- 2023/03/22 06:00
CRDT- 2023/03/07 19:14
PHST- 2022/12/20 00:00 [received]
PHST- 2023/02/28 00:00 [revised]
PHST- 2023/03/02 00:00 [accepted]
PHST- 2023/03/08 06:00 [pubmed]
PHST- 2023/03/22 06:00 [medline]
PHST- 2023/03/07 19:14 [entrez]
AID - S0014-2999(23)00154-1 [pii]
AID - 10.1016/j.ejphar.2023.175643 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 2023 Apr 15;945:175643. doi: 10.1016/j.ejphar.2023.175643. Epub 
      2023 Mar 5.