PMID- 3688822
OWN - NLM
STAT- MEDLINE
DCOM- 19880119
LR  - 20071114
IS  - 0091-7370 (Print)
IS  - 0091-7370 (Linking)
VI  - 17
IP  - 6
DP  - 1987 Nov-Dec
TI  - Videoanalysis of chemokinesis: characterization of speed, persistence and 
      orientation in an agarose assay.
PG  - 389-97
AB  - Time-lapse video recording and off-line computer analysis were used to 
      characterize the chemokinetic behavior of individual human neutrophils migrating 
      in an agarose assay. When neutrophils were stimulated with an isotropic 
      concentration of formyl-methionyl-leucyl-phenylalanine (fMLP), they migrated with 
      a mean speed of 9.6 micron per min and oriented at random. The ratio of net 
      displacement to total distance travelled (persistence of locomotion) was 0.66, 
      indicating that neutrophils maintained some directional persistence even in the 
      absence of a gradient of fMLP. The speed and persistence of locomotion index were 
      correlated because both faster and slower cells had high persistence, while only 
      slower cells had low persistence. The orientation angle was independent of both 
      speed and persistence of locomotion. These are the first reported direct 
      measurements of the chemokinetic locomotion of neutrophils using the agarose 
      assay.
FAU - Burton, J L
AU  - Burton JL
AD  - Department of Pathology and Laboratory Medicine, Medical University of South 
      Carolina, Charleston 29425.
FAU - Bank, H L
AU  - Bank HL
FAU - Law, P
AU  - Law P
LA  - eng
GR  - AM18115/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Ann Clin Lab Sci
JT  - Annals of clinical and laboratory science
JID - 0410247
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 9012-36-6 (Sepharose)
SB  - IM
MH  - *Chemotaxis, Leukocyte
MH  - Computers
MH  - Humans
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*physiology
MH  - Sepharose
MH  - *Video Recording
EDAT- 1987/11/01 00:00
MHDA- 1987/11/01 00:01
CRDT- 1987/11/01 00:00
PHST- 1987/11/01 00:00 [pubmed]
PHST- 1987/11/01 00:01 [medline]
PHST- 1987/11/01 00:00 [entrez]
PST - ppublish
SO  - Ann Clin Lab Sci. 1987 Nov-Dec;17(6):389-97.