PMID- 36890258 OWN - NLM STAT- MEDLINE DCOM- 20230310 LR - 20230310 IS - 1436-5073 (Electronic) IS - 0026-3672 (Linking) VI - 190 IP - 4 DP - 2023 Mar 8 TI - Glucometer-based biosensor for the determination of ractopamine in animal-derived foods using rolling circle amplification. PG - 121 LID - 10.1007/s00604-023-05715-0 [doi] AB - Screening for persistent organic pollutants (POPs) in food is a complex and challenging process, as POPs can be present in very low levels and can be difficult to detect. Herein, we developed an ultrasensitive biosensor based on a rolling circle amplification (RCA) platform using a glucometer to determine POP. The biosensor was constructed using gold nanoparticle probes modified with antibodies and dozens of primers, magnetic microparticle probes conjugated with haptens, and targets. After competition, RCA reactions are triggered, numerous RCA products hybridize with the ssDNA-invertase, and the target is successfully transformed into glucose. Using ractopamine as a model analyte, this strategy obtained a linear detection range of 0.038-5.00 ng mL(-1) and a detection limit of 0.0158 ng mL(-1), which was preliminarily verified by screening in real samples. Compared with conventional immunoassays, this biosensor utilizes the high efficiency of RCA and the portable properties of a glucometer, which effectively improves the sensitivity and simplifies the procedures using magnetic separation technology. Moreover, it has been successfully applied to ractopamine determination in animal-derived foods, revealing its potential as a promising tool for POP screening. CI - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature. FAU - He, Feng AU - He F AD - Institute of Food & Nutrition Science and Technology, Shandong Provincial Key Laboratory of Agro-Products Processing Technology, Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture, Shandong Academy of Agricultural Sciences, Jinan, 250100, China. AD - School of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, 056038, China. FAU - Li, Tengfei AU - Li T AD - School of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, 056038, China. FAU - Wang, Haijie AU - Wang H AD - Institute of Food & Nutrition Science and Technology, Shandong Provincial Key Laboratory of Agro-Products Processing Technology, Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture, Shandong Academy of Agricultural Sciences, Jinan, 250100, China. AD - College of Life Sciences, Yantai University, Yantai, 264005, China. FAU - Du, Pengfei AU - Du P AD - Institute of Food & Nutrition Science and Technology, Shandong Provincial Key Laboratory of Agro-Products Processing Technology, Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture, Shandong Academy of Agricultural Sciences, Jinan, 250100, China. dupengfei2011@163.com. FAU - Wang, Weiting AU - Wang W AD - Institute of Food & Nutrition Science and Technology, Shandong Provincial Key Laboratory of Agro-Products Processing Technology, Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture, Shandong Academy of Agricultural Sciences, Jinan, 250100, China. wangweiting0619@163.com. FAU - Tan, Tianyu AU - Tan T AD - School of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, 056038, China. FAU - Liu, Yaobo AU - Liu Y AD - Institute of Food & Nutrition Science and Technology, Shandong Provincial Key Laboratory of Agro-Products Processing Technology, Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture, Shandong Academy of Agricultural Sciences, Jinan, 250100, China. FAU - Wang, Shoujing AU - Wang S AD - Institute of Food & Nutrition Science and Technology, Shandong Provincial Key Laboratory of Agro-Products Processing Technology, Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture, Shandong Academy of Agricultural Sciences, Jinan, 250100, China. FAU - Ma, Yanli AU - Ma Y AD - Institute of Food & Nutrition Science and Technology, Shandong Provincial Key Laboratory of Agro-Products Processing Technology, Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture, Shandong Academy of Agricultural Sciences, Jinan, 250100, China. FAU - Wang, Yuanshang AU - Wang Y AD - Institute of Food & Nutrition Science and Technology, Shandong Provincial Key Laboratory of Agro-Products Processing Technology, Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture, Shandong Academy of Agricultural Sciences, Jinan, 250100, China. AD - College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. FAU - Hu, Peng AU - Hu P AD - Institute of Food & Nutrition Science and Technology, Shandong Provincial Key Laboratory of Agro-Products Processing Technology, Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture, Shandong Academy of Agricultural Sciences, Jinan, 250100, China. FAU - Abd El-Aty, A M AU - Abd El-Aty AM AD - State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, 250353, China. AD - Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt. AD - Department of Medical Pharmacology, Medical Faculty, Ataturk University, 25240, Erzurum, Turkey. LA - eng GR - ZR2020QC250/Natural Science Foundation of Shandong Province/ GR - CARS-38/Earmarked Fund for China Agriculture Research System/ GR - SDAIT-10-10/Innovation Team Project for Modern Agricultural Industrious Technology System of Shandong Province/ GR - 2021CXGC010809/Key Technology Research and Development Program of Shandong/ GR - 2021TZXD012/Key Technology Research and Development Program of Shandong/ PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20230308 PL - Austria TA - Mikrochim Acta JT - Mikrochimica acta JID - 7808782 RN - 57370OZ3P1 (ractopamine) RN - 7440-57-5 (Gold) RN - 0 (Phenethylamines) SB - IM MH - Animals MH - Gold MH - *Metal Nanoparticles MH - *Biosensing Techniques/methods MH - Phenethylamines OTO - NOTNLM OT - Glucometer OT - Gold nanoparticles OT - Portable sensing OT - Ractopamine OT - Rolling circle amplification EDAT- 2023/03/09 06:00 MHDA- 2023/03/11 06:00 CRDT- 2023/03/08 23:19 PHST- 2022/12/12 00:00 [received] PHST- 2023/02/21 00:00 [accepted] PHST- 2023/03/08 23:19 [entrez] PHST- 2023/03/09 06:00 [pubmed] PHST- 2023/03/11 06:00 [medline] AID - 10.1007/s00604-023-05715-0 [pii] AID - 10.1007/s00604-023-05715-0 [doi] PST - epublish SO - Mikrochim Acta. 2023 Mar 8;190(4):121. doi: 10.1007/s00604-023-05715-0.