PMID- 36891946
OWN - NLM
STAT- MEDLINE
DCOM- 20230417
LR  - 20230417
IS  - 1753-0407 (Electronic)
IS  - 1753-0393 (Print)
IS  - 1753-0407 (Linking)
VI  - 15
IP  - 4
DP  - 2023 Apr
TI  - U-shaped association between serum IGF2BP3 and T2DM: A cross-sectional study in 
      Chinese population.
PG  - 349-361
LID - 10.1111/1753-0407.13378 [doi]
AB  - OBJECTIVE: To clarify the expression of N6-methyladenosine (m(6) A) modulators 
      involved in the pathogenesis of type 2 diabetes mellitus (T2DM). We further 
      explored the association of serum insulin-like growth factor 2 mRNA-binding 
      proteins 3 (IGF2BP3) levels and odds of T2DM in a high-risk population. METHODS: 
      The gene expression data set GSE25724 was obtained from the Gene Expression 
      Omnibus, and a cluster heatmap was generated by using the R package 
      ComplexHeatmap. Differential expression analysis for 13 m(6) A RNA methylation 
      regulators between nondiabetic controls and T2DM subjects was performed using an 
      unpaired t test. A cross-sectional design, including 393 subjects (131 patients 
      with newly diagnosed T2DM, 131 age- and sex-matched subjects with prediabetes, 
      and 131 healthy controls), was carried out. The associations between serum 
      IGF2BP3 concentrations and T2DM were modeled by restricted cubic spline and 
      logistic regression models. RESULTS: Two upregulated (IGF2BP2 and IGF2BP3) and 5 
      downregulated (methyltransferase-like 3 [METTL3], alkylation repair homolog 
      protein 1 [ALKBH1], YTH domain family 2 [YTHDF2], YTHDF3, and heterogeneous 
      nuclear ribonucleoprotein [HNRNPC]) m(6) A-related genes were found in islet 
      samples of T2DM patients. A U-shaped association existed between serum IGF2BP3 
      levels and odds of T2DM according to cubic natural spline analysis models, after 
      adjustment for body mass index, waist circumference, diastolic blood pressure, 
      total cholesterol, and triglyeride. Multivariate logistic regression showed that 
      progressively higher odds of T2DM were observed when serum IGF2BP3 levels were 
      below 0.62 ng/mL (odds ratio 3.03 [95% confidence interval 1.23-7.47]) in model 
      4. CONCLUSION: Seven significantly altered m(6) A RNA methylation genes were 
      identified in T2DM. There was a U-shaped association between serum IGF2BP3 levels 
      and odds of T2DM in the general Chinese adult population. This study provides 
      important evidence for further examination of the role of m(6) A RNA methylation, 
      especially serum IGF2BP3 in T2DM risk assessment.
CI  - (c) 2023 The Authors. Journal of Diabetes published by Ruijin Hospital, Shanghai 
      JiaoTong University School of Medicine and John Wiley & Sons Australia, Ltd.
FAU - Wu, Xiaoying
AU  - Wu X
AD  - Department of Endocrinology, Sun Yat-sen Memorial Hospital, Sun Yat-sen 
      University, Guangzhou, People's Republic of China.
AD  - Department of Endocrinology, National Center of Gerontology, Beijing Hospital, 
      Peking University Fifth School of Clinical Medicine, Beijing, China.
FAU - Wang, Wei
AU  - Wang W
AD  - Department of Endocrinology, Sun Yat-sen Memorial Hospital, Sun Yat-sen 
      University, Guangzhou, People's Republic of China.
FAU - Fan, Shujin
AU  - Fan S
AD  - Department of Endocrinology, Sun Yat-sen Memorial Hospital, Sun Yat-sen 
      University, Guangzhou, People's Republic of China.
FAU - You, Lili
AU  - You L
AD  - Department of Endocrinology, Sun Yat-sen Memorial Hospital, Sun Yat-sen 
      University, Guangzhou, People's Republic of China.
FAU - Li, Feng
AU  - Li F
AD  - Department of Endocrinology, Sun Yat-sen Memorial Hospital, Sun Yat-sen 
      University, Guangzhou, People's Republic of China.
FAU - Zhang, Xiaoyun
AU  - Zhang X
AD  - Department of Endocrinology, Sun Yat-sen Memorial Hospital, Sun Yat-sen 
      University, Guangzhou, People's Republic of China.
FAU - Wu, Hongshi
AU  - Wu H
AD  - Department of Endocrinology, Sun Yat-sen Memorial Hospital, Sun Yat-sen 
      University, Guangzhou, People's Republic of China.
FAU - Tang, Juying
AU  - Tang J
AD  - Department of Endocrinology, Sun Yat-sen Memorial Hospital, Sun Yat-sen 
      University, Guangzhou, People's Republic of China.
FAU - Qi, Yiqin
AU  - Qi Y
AD  - Department of Endocrinology, Sun Yat-sen Memorial Hospital, Sun Yat-sen 
      University, Guangzhou, People's Republic of China.
FAU - Feng, Wanting
AU  - Feng W
AD  - Department of Endocrinology, Sun Yat-sen Memorial Hospital, Sun Yat-sen 
      University, Guangzhou, People's Republic of China.
FAU - Yan, Li
AU  - Yan L
AD  - Department of Endocrinology, Sun Yat-sen Memorial Hospital, Sun Yat-sen 
      University, Guangzhou, People's Republic of China.
FAU - Ren, Meng
AU  - Ren M
AUID- ORCID: 0000-0001-9935-1449
AD  - Department of Endocrinology, Sun Yat-sen Memorial Hospital, Sun Yat-sen 
      University, Guangzhou, People's Republic of China.
LA  - eng
GR  - 81900752/National Natural Science Foundation of China/
GR  - U20A20352/National Natural Science Foundation of China/
GR  - 2020B1111170009/Guangdong Clinical Research Center for Metabolic Diseases/
GR  - 2019A1515011199/Natural Science Foundation of Guangdong Province/
PT  - Journal Article
DEP - 20230309
PL  - Australia
TA  - J Diabetes
JT  - Journal of diabetes
JID - 101504326
RN  - EC 1.14.11.33 (AlkB Homolog 1, Histone H2a Dioxygenase)
RN  - EC 1.14.11.33 (ALKBH1 protein, human)
RN  - 0 (IGF2BP2 protein, human)
RN  - EC 2.1.1.- (Methyltransferases)
RN  - EC 2.1.1.62 (METTL3 protein, human)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (IGF2BP3 protein, human)
SB  - IM
MH  - Adult
MH  - Humans
MH  - AlkB Homolog 1, Histone H2a Dioxygenase/metabolism
MH  - Cross-Sectional Studies
MH  - *Diabetes Mellitus, Type 2
MH  - East Asian People
MH  - Methyltransferases/genetics/metabolism
MH  - Risk Factors
MH  - *RNA-Binding Proteins/blood
PMC - PMC10101838
OTO - NOTNLM
OT  - 2型糖尿病
OT  - N6 -甲基腺苷(m6A)
OT  - N6-methyladenosine (m6A) RNA methylation
OT  - RNA甲基化
OT  - gene expression omnibus
OT  - insulin-like growth factor 2 mRNA-binding protein (IGF2BP)3
OT  - type 2 diabetes mellitus
OT  - 基因表达数据库
OT  - 胰岛素样生长因子2 mRNA结合蛋白3 (IGF2BP3)
COIS- The authors have nothing to disclose.
EDAT- 2023/03/10 06:00
MHDA- 2023/04/17 06:41
PMCR- 2023/03/09
CRDT- 2023/03/09 05:32
PHST- 2023/02/03 00:00 [revised]
PHST- 2022/09/12 00:00 [received]
PHST- 2023/02/20 00:00 [accepted]
PHST- 2023/04/17 06:41 [medline]
PHST- 2023/03/10 06:00 [pubmed]
PHST- 2023/03/09 05:32 [entrez]
PHST- 2023/03/09 00:00 [pmc-release]
AID - JDB13378 [pii]
AID - 10.1111/1753-0407.13378 [doi]
PST - ppublish
SO  - J Diabetes. 2023 Apr;15(4):349-361. doi: 10.1111/1753-0407.13378. Epub 2023 Mar 
      9.