PMID- 36895397
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230311
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 9
IP  - 3
DP  - 2023 Mar
TI  - Development and validation of a novel HPLC-UV method for simultaneous 
      determination of azathioprine metabolites in human red blood cells.
PG  - e13870
LID - 10.1016/j.heliyon.2023.e13870 [doi]
LID - e13870
AB  - A rapid, specific and accurate high-performance liquid chromatography with 
      tunable ultraviolet detection method was developed to simultaneously determine 
      azathioprine metabolites, 6-thioguanine nucleotides (6-TGN) and 6-methyl 
      mercaptopurine riboside (6-MMPr) in human red blood cells. Erythrocyte lysate 
      sample was precipitated by perchloric acid under the protection of 
      dithiothreitol, with 6-TGN and 6-MMPr being acid hydrolyzed to produce 
      6-thioguanine (6-TG) and 6-methymercaptopurine (6-MMP). A Waters Cortecs C(18) 
      column (2.1 x 150 mm, 2.7 mum) was used for chromatographic separation with a 
      water (containing 0.01 mol/L ammonium acetate and 0.2% acetic acid)/methanol 
      linear gradient at a flow rate of 0.45 mL/min in a 5.5 min. UV detection 
      wavelengths were 340 nm for 6-TG, 303 nm for 6-MMP and the IS (5-bromouracil). 
      The calibration curves fitted a least squares model (weighed 1/x (2)) from 0.15 
      to 15 mumol/L for 6-TG (r (2)  = 0.9999) and from 1 to 100 mumol/L for 6-MMP (r (2) 
       = 0.9998). This method was validated according to the FDA bioanalytical method 
      validation guidance and ICH M10 bioanalytical method validation and study sample 
      analysis guidance for industry, and successfully utilized in ten IBD patients 
      receiving azathioprine therapy.
CI  - (c) 2023 Tongji Hospital, Tongji Medical College, Huazhong University of Science 
      and Technology. Published by Elsevier Ltd.
FAU - Yu, Hengyi
AU  - Yu H
AD  - Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Hubei Wuhan 430030, China.
FAU - Li, Dongyan
AU  - Li D
AD  - Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Hubei Wuhan 430030, China.
FAU - Xiang, Dong
AU  - Xiang D
AD  - Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Hubei Wuhan 430030, China.
FAU - Li, Xiping
AU  - Li X
AD  - Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Hubei Wuhan 430030, China.
FAU - Liu, Lu
AU  - Liu L
AD  - Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Hubei Wuhan 430030, China.
FAU - Liu, Dong
AU  - Liu D
AD  - Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Hubei Wuhan 430030, China.
FAU - Gong, Xuepeng
AU  - Gong X
AD  - Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Hubei Wuhan 430030, China.
LA  - eng
PT  - Journal Article
DEP - 20230221
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC9988558
OTO - NOTNLM
OT  - Azathioprine
OT  - HPLC-UV
OT  - Inflammatory bowel disease
OT  - Therapeutic drug monitoring
EDAT- 2023/03/11 06:00
MHDA- 2023/03/11 06:01
PMCR- 2023/02/21
CRDT- 2023/03/10 02:26
PHST- 2022/10/04 00:00 [received]
PHST- 2023/02/06 00:00 [revised]
PHST- 2023/02/14 00:00 [accepted]
PHST- 2023/03/10 02:26 [entrez]
PHST- 2023/03/11 06:00 [pubmed]
PHST- 2023/03/11 06:01 [medline]
PHST- 2023/02/21 00:00 [pmc-release]
AID - S2405-8440(23)01077-0 [pii]
AID - e13870 [pii]
AID - 10.1016/j.heliyon.2023.e13870 [doi]
PST - epublish
SO  - Heliyon. 2023 Feb 21;9(3):e13870. doi: 10.1016/j.heliyon.2023.e13870. eCollection 
      2023 Mar.