PMID- 36896762
OWN - NLM
STAT- MEDLINE
DCOM- 20230613
LR  - 20230613
IS  - 1860-7314 (Electronic)
IS  - 1860-6768 (Linking)
VI  - 18
IP  - 6
DP  - 2023 Jun
TI  - Biotransformation of ethylene glycol to glycolic acid by Yarrowia lipolytica: A 
      route for poly(ethylene terephthalate) (PET) upcycling.
PG  - e2200521
LID - 10.1002/biot.202200521 [doi]
AB  - Biological recycling of PET waste has been extensively investigated recently to 
      tackle plastic waste pollution, and ethylene glycol (EG) is one of the main 
      building blocks recovered from this process. Wild-type Yarrowia lipolytica IMUFRJ 
      50682 can be a biocatalyst to biodepolymerize PET. Herein, we report its ability 
      to perform oxidative biotransformation of EG into glycolic acid (GA): a higher 
      value-added chemical with varied industrial applications. We found that this 
      yeast tolerates high EG concentrations (up to 2 M) based on maximum 
      non-inhibitory concentration (MNIC) tests. Whole-cell biotransformation assays 
      using resting yeast cells showed GA production uncoupled to cell growth 
      metabolism, and (13) C nuclear magnetic resonance (NMR) analysis confirmed GA 
      production. Moreover, higher agitation speed (450 vs. 350 rpm) resulted in a 
      1.12-fold GA production improvement (from 352 to 429.5 mM) during Y. lipolytica 
      cultivation in bioreactors after 72 h. GA was constantly accumulated in the 
      medium, suggesting that this yeast may also share an incomplete oxidation pathway 
      (i.e., it is not metabolized to carbon dioxide) as seen in acetic acid bacterial 
      group. Additional assays using higher chain-length diols (1,3-propanediol, 
      1,4-butanediol, and 1,6-hexanediol) revealed that C4 and C6 diols were more 
      cytotoxic, suggesting that they underwent different pathways in the cells. We 
      found that this yeast consumed extensively all these diols, however, (13) C NMR 
      analysis from supernatant identified solely the presence of 4-hydroxybutanoic 
      acid from 1,4-butanediol, along with GA from EG oxidation. Findings reported 
      herein reveal a potential route for PET upcycling to a higher value-added 
      product.
CI  - (c) 2023 Wiley-VCH GmbH.
FAU - Carniel, Adriano
AU  - Carniel A
AUID- ORCID: 0000-0002-9675-4813
AD  - Department of Biochemical Engineering, Escola de Quimica, Universidade Federal do 
      Rio de Janeiro (UFRJ) - Cidade Universitaria, Rio de Janeiro, RJ, Brazil.
FAU - Santos, Ariane Gaspar
AU  - Santos AG
AUID- ORCID: 0000-0001-7803-9827
AD  - Department of Biochemical Engineering, Escola de Quimica, Universidade Federal do 
      Rio de Janeiro (UFRJ) - Cidade Universitaria, Rio de Janeiro, RJ, Brazil.
FAU - Chinelatto, Luiz Silvino Junior
AU  - Chinelatto LS Junior
AUID- ORCID: 0000-0002-0974-0465
AD  - Petrobras Research, Development and Innovation Center (Cenpes), sAv. Horacio 
      Macedo, Cidade Universitaria, Rio de Janeiro, RJ, Brazil.
FAU - Castro, Aline M
AU  - Castro AM
AUID- ORCID: 0000-0002-1355-8455
AD  - Petrobras Research, Development and Innovation Center (Cenpes), sAv. Horacio 
      Macedo, Cidade Universitaria, Rio de Janeiro, RJ, Brazil.
FAU - Coelho, Maria Alice Zarur
AU  - Coelho MAZ
AUID- ORCID: 0000-0002-0814-3626
AD  - Department of Biochemical Engineering, Escola de Quimica, Universidade Federal do 
      Rio de Janeiro (UFRJ) - Cidade Universitaria, Rio de Janeiro, RJ, Brazil.
LA  - eng
GR  - 2016/0194-8/Petrobras/
PT  - Journal Article
DEP - 20230417
PL  - Germany
TA  - Biotechnol J
JT  - Biotechnology journal
JID - 101265833
RN  - FC72KVT52F (Ethylene Glycol)
RN  - 7XOO2LE6G3 (1,4-butanediol)
RN  - 0WT12SX38S (glycolic acid)
RN  - 6S7NKZ40BQ (terephthalic acid)
RN  - 0 (Ethylenes)
SB  - IM
MH  - *Ethylene Glycol/metabolism
MH  - *Yarrowia/metabolism
MH  - Biotransformation
MH  - Ethylenes/metabolism
OTO - NOTNLM
OT  - PET upcycling
OT  - Yarrowia lipolytica
OT  - biotransformation
OT  - carboxylic acid
OT  - ethylene glycol
OT  - glycolic acid
EDAT- 2023/03/11 06:00
MHDA- 2023/06/13 06:42
CRDT- 2023/03/10 05:33
PHST- 2023/01/25 00:00 [revised]
PHST- 2022/10/15 00:00 [received]
PHST- 2023/02/22 00:00 [accepted]
PHST- 2023/06/13 06:42 [medline]
PHST- 2023/03/11 06:00 [pubmed]
PHST- 2023/03/10 05:33 [entrez]
AID - 10.1002/biot.202200521 [doi]
PST - ppublish
SO  - Biotechnol J. 2023 Jun;18(6):e2200521. doi: 10.1002/biot.202200521. Epub 2023 Apr 
      17.