PMID- 36899278
OWN - NLM
STAT- MEDLINE
DCOM- 20230501
LR  - 20230501
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 50
IP  - 5
DP  - 2023 May
TI  - miR-122 dysregulation is associated with type 2 diabetes mellitus-induced 
      dyslipidemia and hyperglycemia independently of its rs17669 variant.
PG  - 4217-4224
LID - 10.1007/s11033-023-08344-1 [doi]
AB  - BACKGROUND: miR-122 is a liver specific micro-RNA that participates in the 
      regulation of carbohydrate and lipid metabolism. The rs17669 variant of miR-122 
      is positioned at the flanking region of miR-122 and may affect its stability and 
      maturation. Therefore, this study was aimed to investigate the association of the 
      rs17669 polymorphism with the miR-122 circulating level, risk of type 2 diabetes 
      mellitus (T2DM) development, and biochemical parameters in T2DM patients and 
      matched healthy controls. METHODS AND RESULTS: This study involved 295 subjects 
      (controls: n = 145 and T2DM: n = 150). The rs17669 variant genotyping was done by 
      ARMS-PCR. Serum biochemical parameters including lipid profile, small-dense low 
      density lipoprotein (sdLDL) and glucose were measured by colorimetric kits. 
      Insulin and Glycated hemoglobin (HbA1c) were assayed using ELISA and capillary 
      electrophoresis methods, respectively. miR-122 expression was measured by 
      real-time PCR. There was no significant difference between study groups in terms 
      of allele and genotype distribution (P > 0.05). The rs17669 variant did not have 
      any significant association with miR-122 gene expression and biochemical 
      parameters (P > 0.05). miR-122 expression level in T2DM patients was 
      significantly higher than that in control subjects (5.7 +/- 2.4 vs. 1.4 +/- 0.78) 
      (P < 0.001). Furthermore, miR-122 fold change had a positive and significant 
      correlation with low-density lipoprotein cholesterol (LDL-C), sdLDL, fasting 
      blood sugar (FBS), and insulin resistance (P < 0.05). CONCLUSION: It can be 
      concluded that the rs17669 variant of miR-122 is not associated with the miR-122 
      expression and T2DM-associated serum parameters. Furthermore, it can be suggested 
      that miR-122 dysregulation is involved in T2DM development through inducing 
      dyslipidemia, hyperglycemia, and resistance to insulin.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Mokhtari Ardekani, Abnoos
AU  - Mokhtari Ardekani A
AD  - Endocrinology and Metabolism Research Center, Institute of Basic and Clinical 
      Physiology Science & Physiology Research Center, Kerman University of Medical 
      Sciences, Kerman, Iran.
FAU - Mohammadzadehsaliani, Saman
AU  - Mohammadzadehsaliani S
AD  - Ophthalmology Department, Buraimi Hospital, Buraimi, Oman.
FAU - Behrouj, Hamid
AU  - Behrouj H
AD  - Behbahan Faculty of Medical Sciences, Behbahan, Iran.
FAU - Moridi, Heresh
AU  - Moridi H
AD  - Department of Medical Laboratory Sciences, Faculty of Paramedical Sciences, 
      Kurdistan University of Medical Sciences, Sanandaj, Iran.
FAU - Moradi, Mohammad Nabi
AU  - Moradi MN
AD  - Department of Clinical Laboratory Sciences, School of Allied Medicine, Lorestan 
      University of Medical Sciences, Khorramabad, Iran, Lorestan University of Medical 
      Sciences, Lorestan, Iran.
FAU - Ghasemi, Hassan
AU  - Ghasemi H
AUID- ORCID: 0000-0001-7809-2732
AD  - Department of Clinical Biochemistry, Abadan University of Medical Sciences, 
      Abadan, Iran. clinicalbiochem.1365@gmail.com.
LA  - eng
GR  - 97U-355/Abadan University of Medical Sciences/
PT  - Journal Article
DEP - 20230310
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (MicroRNAs)
RN  - 0 (Insulin)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Blood Glucose)
RN  - 0 (MIRN122 microRNA, human)
SB  - IM
MH  - Humans
MH  - *Diabetes Mellitus, Type 2
MH  - *Hyperglycemia/genetics
MH  - *MicroRNAs/genetics
MH  - Insulin
MH  - Lipoproteins, LDL
MH  - *Dyslipidemias/genetics
MH  - Blood Glucose/metabolism
OTO - NOTNLM
OT  - Insulin resistance
OT  - Type 2 diabetes mellitus
OT  - miR-122
OT  - rs17669
EDAT- 2023/03/11 06:00
MHDA- 2023/05/01 06:42
CRDT- 2023/03/10 23:28
PHST- 2022/12/31 00:00 [received]
PHST- 2023/02/15 00:00 [accepted]
PHST- 2023/05/01 06:42 [medline]
PHST- 2023/03/11 06:00 [pubmed]
PHST- 2023/03/10 23:28 [entrez]
AID - 10.1007/s11033-023-08344-1 [pii]
AID - 10.1007/s11033-023-08344-1 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2023 May;50(5):4217-4224. doi: 10.1007/s11033-023-08344-1. Epub 
      2023 Mar 10.