PMID- 36899801
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230313
IS  - 2076-2615 (Print)
IS  - 2076-2615 (Electronic)
IS  - 2076-2615 (Linking)
VI  - 13
IP  - 5
DP  - 2023 Mar 6
TI  - Molecular Cytogenetics in Domestic Bovids: A Review.
LID - 10.3390/ani13050944 [doi]
LID - 944
AB  - The discovery of the Robertsonian translocation (rob) involving cattle 
      chromosomes 1 and 29 and the demonstration of its deleterious effects on 
      fertility focused the interest of many scientific groups on using chromosome 
      banding techniques to reveal chromosome abnormalities and verify their effects on 
      fertility in domestic animals. At the same time, comparative banding studies 
      among various species of domestic or wild animals were found useful for 
      delineating chromosome evolution among species. The advent of molecular 
      cytogenetics, particularly the use of fluorescence in situ hybridization (FISH), 
      has allowed a deeper investigation of the chromosomes of domestic animals 
      through: (a) the physical mapping of specific DNA sequences on chromosome 
      regions; (b) the use of specific chromosome markers for the identification of the 
      chromosomes or chromosome regions involved in chromosome abnormalities, 
      especially when poor banding patterns are produced; (c) better anchoring of 
      radiation hybrid and genetic maps to specific chromosome regions; (d) better 
      comparisons of related and unrelated species by comparative FISH mapping and/or 
      Zoo-FISH techniques; (e) the study of meiotic segregation, especially by 
      sperm-FISH, in some chromosome abnormalities; (f) better demonstration of 
      conserved or lost DNA sequences in chromosome abnormalities; (g) the use of 
      informatic and genomic reconstructions, in addition to CGH arrays, to predict 
      conserved or lost chromosome regions in related species; and (h) the study of 
      some chromosome abnormalities and genomic stability using PCR applications. This 
      review summarizes the most important applications of molecular cytogenetics in 
      domestic bovids, with an emphasis on FISH mapping applications.
FAU - Iannuzzi, Alessandra
AU  - Iannuzzi A
AUID- ORCID: 0000-0002-2693-3111
AD  - Institute for Animal Production System in Mediterranean Environment, National 
      Research Council, 80055 Portici, Italy.
FAU - Iannuzzi, Leopoldo
AU  - Iannuzzi L
AUID- ORCID: 0000-0002-5445-883X
AD  - Institute for Animal Production System in Mediterranean Environment, National 
      Research Council, 80055 Portici, Italy.
FAU - Parma, Pietro
AU  - Parma P
AD  - Department of Agricultural and Environmental Sciences, University of Milan, 20133 
      Milan, Italy.
LA  - eng
GR  - 486 GENOBU/PON1/
PT  - Journal Article
PT  - Review
DEP - 20230306
PL  - Switzerland
TA  - Animals (Basel)
JT  - Animals : an open access journal from MDPI
JID - 101635614
PMC - PMC10000107
OTO - NOTNLM
OT  - FISH mapping
OT  - PCR
OT  - animal cytogenetics
OT  - cattle
OT  - goat
OT  - river buffalo
OT  - sheep
COIS- The authors declare no conflict of interest.
EDAT- 2023/03/12 06:00
MHDA- 2023/03/12 06:01
PMCR- 2023/03/06
CRDT- 2023/03/11 01:03
PHST- 2023/01/31 00:00 [received]
PHST- 2023/02/28 00:00 [revised]
PHST- 2023/03/02 00:00 [accepted]
PHST- 2023/03/11 01:03 [entrez]
PHST- 2023/03/12 06:00 [pubmed]
PHST- 2023/03/12 06:01 [medline]
PHST- 2023/03/06 00:00 [pmc-release]
AID - ani13050944 [pii]
AID - animals-13-00944 [pii]
AID - 10.3390/ani13050944 [doi]
PST - epublish
SO  - Animals (Basel). 2023 Mar 6;13(5):944. doi: 10.3390/ani13050944.