PMID- 36901754
OWN - NLM
STAT- MEDLINE
DCOM- 20230314
LR  - 20240109
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 5
DP  - 2023 Feb 21
TI  - Transcriptome Analysis of Retinal and Choroidal Pathologies in Aged BALB/c Mice 
      Following Systemic Neonatal Murine Cytomegalovirus Infection.
LID - 10.3390/ijms24054322 [doi]
LID - 4322
AB  - Our previous studies have shown that systemic neonatal murine cytomegalovirus 
      (MCMV) infection of BALB/c mice spread to the eye with subsequent establishment 
      of latency in choroid/RPE. In this study, RNA sequencing (RNA-Seq) analysis was 
      used to determine the molecular genetic changes and pathways affected by ocular 
      MCMV latency. MCMV (50 pfu per mouse) or medium as control were injected 
      intra-peritoneally (i.p.) into BALB/c mice at <3 days after birth. At 18 months 
      post injection, the mice were euthanized, and the eyes were collected and 
      prepared for RNA-Seq. Compared to three uninfected control eyes, we identified 
      321 differentially expressed genes (DEGs) in six infected eyes. Using the QIAGEN 
      Ingenuity Pathway Analysis (QIAGEN IPA), we identified 17 affected canonical 
      pathways, 10 of which function in neuroretinal signaling, with the majority of 
      DEGs being downregulated, while 7 pathways function in upregulated 
      immune/inflammatory responses. Retinal and epithelial cell death pathways 
      involving both apoptosis and necroptosis were also activated. MCMV ocular latency 
      is associated with upregulation of immune and inflammatory responses and 
      downregulation of multiple neuroretinal signaling pathways. Cell death signaling 
      pathways are also activated and contribute to the degeneration of photoreceptors, 
      RPE, and choroidal capillaries.
FAU - Zhang, Xinyan
AU  - Zhang X
AD  - Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta 
      University, Augusta, GA 30912, USA.
AD  - James and Jean Vision Discovery Institute, Medical College of Georgia, Augusta 
      University, Augusta, GA 30912, USA.
FAU - Xu, Jinxian
AU  - Xu J
AD  - Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta 
      University, Augusta, GA 30912, USA.
AD  - James and Jean Vision Discovery Institute, Medical College of Georgia, Augusta 
      University, Augusta, GA 30912, USA.
FAU - Marshall, Brendan
AU  - Marshall B
AD  - Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta 
      University, Augusta, GA 30912, USA.
FAU - Dong, Zheng
AU  - Dong Z
AUID- ORCID: 0000-0003-3538-8095
AD  - Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta 
      University, Augusta, GA 30912, USA.
AD  - Charlie Norwood VA Medical Center, Augusta, GA 30904, USA.
FAU - Liu, Yutao
AU  - Liu Y
AUID- ORCID: 0000-0003-2870-4504
AD  - Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta 
      University, Augusta, GA 30912, USA.
AD  - James and Jean Vision Discovery Institute, Medical College of Georgia, Augusta 
      University, Augusta, GA 30912, USA.
AD  - Center for Biotechnology and Genomic Medicine, Medical College of Georgia, 
      Augusta University, Augusta, GA 30912, USA.
FAU - Espinosa-Heidmann, Diego G
AU  - Espinosa-Heidmann DG
AD  - James and Jean Vision Discovery Institute, Medical College of Georgia, Augusta 
      University, Augusta, GA 30912, USA.
AD  - Department of Ophthamology, Medical College of Georgia, Augusta University, 
      Augusta, GA 30912, USA.
FAU - Zhang, Ming
AU  - Zhang M
AUID- ORCID: 0000-0002-4012-3932
AD  - Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta 
      University, Augusta, GA 30912, USA.
AD  - James and Jean Vision Discovery Institute, Medical College of Georgia, Augusta 
      University, Augusta, GA 30912, USA.
LA  - eng
GR  - P30 EY031631/EY/NEI NIH HHS/United States
GR  - R01 EY023242/EY/NEI NIH HHS/United States
GR  - R21 EY028671/EY/NEI NIH HHS/United States
GR  - RO1 EY026642/EY/NEI NIH HHS/United States
PT  - Journal Article
DEP - 20230221
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
SB  - IM
MH  - Mice
MH  - Animals
MH  - Mice, Inbred BALB C
MH  - *Eye Infections, Viral/metabolism/pathology
MH  - *Cytomegalovirus Infections
MH  - Choroid/metabolism
MH  - *Muromegalovirus/physiology
MH  - Gene Expression Profiling
PMC - PMC10001583
OTO - NOTNLM
OT  - RNA sequencing
OT  - age-related macular degeneration
OT  - cytomegalovirus
OT  - degeneration
OT  - inflammation
OT  - latency
COIS- The authors declare no conflict of interest.
EDAT- 2023/03/12 06:00
MHDA- 2023/03/15 06:00
PMCR- 2023/02/21
CRDT- 2023/03/11 01:15
PHST- 2023/01/25 00:00 [received]
PHST- 2023/02/16 00:00 [revised]
PHST- 2023/02/17 00:00 [accepted]
PHST- 2023/03/11 01:15 [entrez]
PHST- 2023/03/12 06:00 [pubmed]
PHST- 2023/03/15 06:00 [medline]
PHST- 2023/02/21 00:00 [pmc-release]
AID - ijms24054322 [pii]
AID - ijms-24-04322 [pii]
AID - 10.3390/ijms24054322 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Feb 21;24(5):4322. doi: 10.3390/ijms24054322.