PMID- 36902060
OWN - NLM
STAT- MEDLINE
DCOM- 20230410
LR  - 20230410
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 5
DP  - 2023 Feb 27
TI  - Isocorydine Ameliorates IL-6 Expression in Bone Marrow-Derived Macrophages and 
      Acute Lung Injury Induced by Lipopolysaccharide.
LID - 10.3390/ijms24054629 [doi]
LID - 4629
AB  - Isocorydine (ICD) is a type of isoquinoline alkaloid originating from Corydalis 
      edulis, which has been used to relieve spasm, dilate blood vessels, and treat 
      malaria as well as hypoxia in clinic. However, its effect on inflammation and 
      underlying mechanisms remains unclear. The aim of our study was to determine the 
      potential effects and mechanisms of ICD on pro-inflammatory interleukin-6 (IL-6) 
      expression in bone marrow-derived macrophages (BMDMs) and acute lung injury mouse 
      model. A mouse model of acute lung injury was established by intraperitoneal 
      injection of LPS and treated with different doses of ICD. The body weight and 
      food intake of mice were monitored to determine the toxicity of ICD. The tissue 
      samples of lung, spleen and blood were taken to assess the pathological symptoms 
      of acute lung injury and the expression levels of IL-6. Further, BMDMs isolated 
      from C57BL/6 mice were cultured in vitro and treated with granulocyte-macrophage 
      colony-stimulating factor (GM-CSF), LPS and different doses of ICD. CCK-8 assay 
      and flow cytometry were performed to assess the viability of BMDMs. The 
      expression of IL-6 was detected by RT-PCR and ELISA. RNA-seq was carried out to 
      detect the differential expression genes of ICD-treated BMDMs. Western blotting 
      was used to detect the change in MAPK and NF-kappaB signaling pathways. Our findings 
      show that ICD ameliorates IL-6 expression and attenuates phosphorylation of p65 
      and JNK in BMDMs, and can protect mice from acute lung injury.
FAU - Tu, Yifan
AU  - Tu Y
AD  - Key Laboratory of Cancer Prevention and Intervention of China National Ministry 
      of Education, Cancer Institute, Second Affiliated Hospital, Zhejiang University 
      School of Medicine, 88 Jiefang Road, Hangzhou 310009, China.
AD  - Institute of Genetics, Department of Human Genetics, Zhejiang University School 
      of Medicine, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
FAU - Li, Xiaodong
AU  - Li X
AD  - Institute of Genetics, Department of Human Genetics, Zhejiang University School 
      of Medicine, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
FAU - Fu, Yuanzheng
AU  - Fu Y
AD  - Institute of Genetics, Department of Human Genetics, Zhejiang University School 
      of Medicine, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
FAU - Chen, Yunyun
AU  - Chen Y
AD  - Institute of Genetics, Department of Human Genetics, Zhejiang University School 
      of Medicine, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
FAU - Fang, Hui
AU  - Fang H
AD  - Institute of Genetics, Department of Human Genetics, Zhejiang University School 
      of Medicine, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
FAU - Li, Yuan
AU  - Li Y
AD  - Institute of Genetics, Department of Human Genetics, Zhejiang University School 
      of Medicine, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
FAU - Gu, Ying
AU  - Gu Y
AD  - Key Laboratory of Cancer Prevention and Intervention of China National Ministry 
      of Education, Cancer Institute, Second Affiliated Hospital, Zhejiang University 
      School of Medicine, 88 Jiefang Road, Hangzhou 310009, China.
AD  - Institute of Genetics, Department of Human Genetics, Zhejiang University School 
      of Medicine, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China.
FAU - Zhang, Jiawei
AU  - Zhang J
AD  - Key Laboratory of Cancer Prevention and Intervention of China National Ministry 
      of Education, Cancer Institute, Second Affiliated Hospital, Zhejiang University 
      School of Medicine, 88 Jiefang Road, Hangzhou 310009, China.
LA  - eng
GR  - 32070630/National Natural Science Foundation of China/
GR  - 32100477/National Natural Science Foundation of China/
GR  - 2019KY574/Medical Health Science and Technology Project of Zhejiang Provincial 
      Health Commission/
GR  - 2020R01006/the Zhejiang Innovation Team Grant/
PT  - Journal Article
DEP - 20230227
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Interleukin-6)
RN  - 3B5E87JEOX (isocorydine)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Aporphines)
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Acute Lung Injury/metabolism
MH  - *Interleukin-6/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Lung/pathology
MH  - Macrophages/metabolism
MH  - Mice, Inbred C57BL
MH  - NF-kappa B/metabolism
MH  - *Aporphines/pharmacology
MH  - *Anti-Inflammatory Agents, Non-Steroidal/pharmacology
PMC - PMC10003757
OTO - NOTNLM
OT  - acute lung injury
OT  - interleukin-6
OT  - isocorydine
OT  - lipopolysaccharide
OT  - macrophages
COIS- The authors declare no conflict of interest.
EDAT- 2023/03/12 06:00
MHDA- 2023/03/15 06:00
PMCR- 2023/02/27
CRDT- 2023/03/11 01:17
PHST- 2022/12/17 00:00 [received]
PHST- 2023/02/16 00:00 [revised]
PHST- 2023/02/22 00:00 [accepted]
PHST- 2023/03/11 01:17 [entrez]
PHST- 2023/03/12 06:00 [pubmed]
PHST- 2023/03/15 06:00 [medline]
PHST- 2023/02/27 00:00 [pmc-release]
AID - ijms24054629 [pii]
AID - ijms-24-04629 [pii]
AID - 10.3390/ijms24054629 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Feb 27;24(5):4629. doi: 10.3390/ijms24054629.