PMID- 36903516
OWN - NLM
STAT- MEDLINE
DCOM- 20230316
LR  - 20230316
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 28
IP  - 5
DP  - 2023 Feb 28
TI  - H-rGO-Pd NPs Nanozyme Enhanced Silver Deposition Strategy for Electrochemical 
      Detection of Glypican-3.
LID - 10.3390/molecules28052271 [doi]
LID - 2271
AB  - Glypican-3 (GPC3), as an emerging biomarker, has been shown to be beneficial for 
      the early diagnosis and treatment of hepatocellular carcinoma (HCC). In this 
      study, an ultrasensitive electrochemical biosensor for GPC3 detection has been 
      constructed based on the hemin-reduced graphene oxide-palladium nanoparticles 
      (H-rGO-Pd NPs) nanozyme-enhanced silver deposition signal amplification strategy. 
      When GPC3 specifically interacted with GPC3 antibody (GPC3(Ab)) and GPC3 aptamer 
      (GPC3(Apt)), an "H-rGO-Pd NPs-GPC3(Apt)/GPC3/GPC3(Ab)" sandwich complex was 
      formed with peroxidase-like properties which enhanced H(2)O(2) to reduce the 
      silver (Ag) ions in solution to metallic Ag, resulting in the deposition of 
      silver nanoparticles (Ag NPs) on the surface of the biosensor. The amount of 
      deposited Ag, which was derived from the amount of GPC3, was quantified by the 
      differential pulse voltammetry (DPV) method. Under ideal circumstances, the 
      response value was linearly correlated with GPC3 concentration at 10.0-100.0 
      mug/mL with R(2) of 0.9715. When the GPC3 concentration was in the range from 0.01 
      to 10.0 mug/mL, the response value was logarithmically linear with the GPC3 
      concentration with R(2) of 0.9941. The limit of detection was 3.30 ng/mL at a 
      signal-to-noise ratio of three and the sensitivity was 1.535 muAmuM(-1)cm(-2). 
      Furthermore, the electrochemical biosensor detected the GPC3 level in actual 
      serum samples with good recoveries (103.78-106.52%) and satisfactory relative 
      standard deviations (RSDs) (1.89-8.81%), which confirmed the applicability of the 
      sensor in practical applications. This study provides a new analytical method for 
      measuring the level of GPC3 in the early diagnosis of HCC.
FAU - Li, Guiyin
AU  - Li G
AUID- ORCID: 0000-0001-8356-4618
AD  - Guangxi Key Laboratory of Information Materials, School of Life and Environmental 
      Sciences, Guilin University of Electronic Technology, Guilin 541004, China.
AD  - College of Chemistry, Guangdong University of Petrochemical Technology, Guandu 
      Road, Maoming 525000, China.
FAU - Wang, Bo
AU  - Wang B
AD  - Guangxi Key Laboratory of Information Materials, School of Life and Environmental 
      Sciences, Guilin University of Electronic Technology, Guilin 541004, China.
FAU - Li, Ling
AU  - Li L
AD  - Guangxi Key Laboratory of Information Materials, School of Life and Environmental 
      Sciences, Guilin University of Electronic Technology, Guilin 541004, China.
FAU - Li, Xinhao
AU  - Li X
AD  - Guangxi Key Laboratory of Information Materials, School of Life and Environmental 
      Sciences, Guilin University of Electronic Technology, Guilin 541004, China.
FAU - Yan, Ruijie
AU  - Yan R
AD  - Guangxi Key Laboratory of Information Materials, School of Life and Environmental 
      Sciences, Guilin University of Electronic Technology, Guilin 541004, China.
FAU - Liang, Jintao
AU  - Liang J
AD  - Guangxi Key Laboratory of Information Materials, School of Life and Environmental 
      Sciences, Guilin University of Electronic Technology, Guilin 541004, China.
FAU - Zhou, Xinchun
AU  - Zhou X
AD  - Guangdi Maoming Chemical Co., Ltd., Maoming High-Tech Industrial Development 
      Zone, Maoming 525000, China.
FAU - Li, Liuxun
AU  - Li L
AUID- ORCID: 0000-0001-9762-4183
AD  - Solid Tumour Target Discovery Laboratory, Translational and Clinical Research 
      Institute, Newcastle University Centre for Cancer, Faculty of Medical Sciences, 
      Newcastle University, Newcastle upon Tyne NE2 4HH, UK.
FAU - Zhou, Zhide
AU  - Zhou Z
AD  - Guangxi Key Laboratory of Information Materials, School of Life and Environmental 
      Sciences, Guilin University of Electronic Technology, Guilin 541004, China.
LA  - eng
GR  - Nos. 62161009 and 62261010/the National Nature Science Foundation of China/
GR  - RC-XJ2022000401/Projects of Talents Recruitment of GDUPT/
GR  - No. 211022-K/the Open Fund of Guangxi Key Laboratory of Information Materials/
GR  - No. 20-065-76/the Fund of Guangxi Key laboratory of Metabolic Diseases Research/
PT  - Journal Article
DEP - 20230228
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Glypicans)
RN  - 0 (GPC3 protein, human)
RN  - 0 (graphene oxide)
RN  - 7782-42-5 (Graphite)
RN  - 743LRP9S7N (Hemin)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - 5TWQ1V240M (Palladium)
RN  - 3M4G523W1G (Silver)
SB  - IM
MH  - Humans
MH  - *Biosensing Techniques/methods
MH  - Carcinoma, Hepatocellular
MH  - Electrochemical Techniques/methods
MH  - *Glypicans
MH  - *Graphite/chemistry
MH  - Hemin/chemistry
MH  - Hydrogen Peroxide
MH  - Liver Neoplasms
MH  - *Metal Nanoparticles/chemistry
MH  - Palladium
MH  - Silver/chemistry
PMC - PMC10004744
OTO - NOTNLM
OT  - Glypican-3
OT  - H-rGO-Pd NPs nanozyme
OT  - electrochemical nanobiosensor
OT  - hepatocellular carcinoma
OT  - peroxidase-like catalytic silver deposition
COIS- The authors declare no conflict of interest.
EDAT- 2023/03/12 06:00
MHDA- 2023/03/15 06:00
PMCR- 2023/02/28
CRDT- 2023/03/11 01:26
PHST- 2023/01/05 00:00 [received]
PHST- 2023/02/11 00:00 [revised]
PHST- 2023/02/15 00:00 [accepted]
PHST- 2023/03/11 01:26 [entrez]
PHST- 2023/03/12 06:00 [pubmed]
PHST- 2023/03/15 06:00 [medline]
PHST- 2023/02/28 00:00 [pmc-release]
AID - molecules28052271 [pii]
AID - molecules-28-02271 [pii]
AID - 10.3390/molecules28052271 [doi]
PST - epublish
SO  - Molecules. 2023 Feb 28;28(5):2271. doi: 10.3390/molecules28052271.