PMID- 36905259
OWN - NLM
STAT- MEDLINE
DCOM- 20230314
LR  - 20230314
IS  - 1165-158X (Electronic)
IS  - 0145-5680 (Linking)
VI  - 68
IP  - 9
DP  - 2022 Sep 30
TI  - Investigation on Antimicrobial, Antioxidant, and Anti-cancerous activity of 
      Agaricus bisporus derived beta-Glucan particles against cervical cancer cell line.
PG  - 150-159
LID - 10.14715/cmb/2022.68.9.24 [doi]
AB  - Cervical cancer is one of the leading causes of death among women. Due to 
      incomplete knowledge and hidden symptoms, it is not easily diagnosable. After the 
      diagnosis of cervical cancer at an advanced stage, treatment such as chemotherapy 
      and radiation therapy become too much costly along with having many side effects 
      such as hair loss, loss of appetite, nausea, tiredness, etc. beta-Glucan does a 
      novel polysaccharide has many immunomodulatory properties. In our research, we 
      have tested the efficacy of Agaricus bisporus derived beta-Glucan particles (ADGPs) 
      as an antimicrobial, antioxidant and anticancer agent against the cervical cancer 
      HeLa cells. Prepared particles were quantified for carbohydrate content by 
      anthrone test and further HPTLC analysis to confirm the polysaccharide nature and 
      1,3 glycosidic linkages of beta-Glucan. ADGPs were found to have efficient 
      antimicrobial activity against various fungal and bacterial tested strains. DPPH 
      assay confirmed the antioxidant activity of ADGPs. Cell viability was assessed 
      against the cervical cancer cell line by using the MTT and IC50 was found at 
      54mug/ml. Furthermore, beta-Glucan was found to induce a significant amount of ROS, 
      leading to the apoptosis of cells. The same was also assessed with the help of 
      Propidium Iodide (PI) staining. With the help of JC-1 staining, beta-Glucan was 
      found to disrupt the Mitochondrial Membrane Potential (MMP), ultimately resulting 
      in the cancer cell HeLa death. Based on our experimental findings, we found that 
      ADGPs can be proven as an efficient therapy for cervical cancer treatment and 
      work as an antimicrobial and antioxidant agent.
FAU - Ankita Bhosale
AU  - Ankita Bhosale
AD  - Department of Biotechnology, Parul Institute of Applied Sciences and Centre of 
      Research for Development, Parul University, Vadodara, Gujarat, 391760, India. 
      ankitabhosale1682@gmail.com.
FAU - Rashmi Trivedi
AU  - Rashmi Trivedi
AD  - Department of Biotechnology, Parul Institute of Applied Sciences and Centre of 
      Research for Development, Parul University, Vadodara, Gujarat, 391760, India. 
      rashmitrivedi888@gmail.com.
FAU - Tarun Kumar Upadhyay
AU  - Tarun Kumar Upadhyay
AD  - Department of Biotechnology, Parul Institute of Applied Sciences and Centre of 
      Research for Development, Parul University, Vadodara, Gujarat, 391760, India. 
      tarun_bioinfo@yahoo.co.in.
FAU - Dhruvi Gurjar
AU  - Dhruvi Gurjar
AD  - Department of Biotechnology, Parul Institute of Applied Sciences and Centre of 
      Research for Development, Parul University, Vadodara, Gujarat, 391760, India. 
      dhruvigurjar00@gmail.com.
FAU - Faranak Aghaz
AU  - Faranak Aghaz
AD  - Nano Drug Delivery Research Center, Faculty of Pharmacy, Health Technology 
      Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran. 
      faranak.aghaz@kums.ac.ir.
FAU - Fahad Khan
AU  - Fahad Khan
AD  - Department of Biotechnology, Noida Institute of Engineering & Technology, Greater 
      Noida, 201306, India. fahadintegralian@gmail.com.
FAU - Pratibha Pandey
AU  - Pratibha Pandey
AD  - Department of Biotechnology, Noida Institute of Engineering & Technology, Greater 
      Noida, 201306, India. shukla.pratibha1985@gmail.com.
FAU - Zeyaullah, Md
AU  - Zeyaullah M
AD  - Department of Basic Medical Science, College of Applied Medical Sciences, Khamis 
      Mushayt Campus, King Khalid University (KKU), Abha, KSA. mdhafed@kku.edu.sa.
FAU - Alam, Md Shane
AU  - Alam MS
AD  - Department of Medical Laboratory Technology, College of Applied Medical Sciences, 
      Jazan University, Jazan, KSA. mshanealam8@gmail.com.
FAU - Al-Najjar, Mohammad A A
AU  - Al-Najjar MAA
AD  - Department of pharmaceutical sciences and pharmaceutics. Faculty of Pharmacy, 
      Applied Science Private University, Amman. Jordan. moh_alnajjar@asu.edu.Jo.
FAU - Samra Siddiqui
AU  - Samra Siddiqui
AD  - Department Health Services Management, College of Public Health and Health 
      Informatics, University of Hail, Hail P.O. Box 2240, Saudi Arabia. 
      s.siddiqui@uoh.edu.sa.
LA  - eng
PT  - Journal Article
DEP - 20220930
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - 0 (Glucans)
RN  - 0 (beta-Glucans)
RN  - 0 (Antioxidants)
RN  - 0 (Polysaccharides)
RN  - 0 (Anti-Infective Agents)
RN  - Agaricus bisporus
SB  - IM
MH  - Humans
MH  - Female
MH  - Glucans
MH  - *beta-Glucans/analysis/pharmacology/therapeutic use
MH  - *Uterine Cervical Neoplasms/drug therapy
MH  - Antioxidants/pharmacology
MH  - HeLa Cells
MH  - Polysaccharides/pharmacology
MH  - *Anti-Infective Agents/pharmacology
MH  - Apoptosis
EDAT- 2023/03/12 06:00
MHDA- 2023/03/15 06:00
CRDT- 2023/03/11 06:11
PHST- 2022/10/16 00:00 [received]
PHST- 2023/03/11 06:11 [entrez]
PHST- 2023/03/12 06:00 [pubmed]
PHST- 2023/03/15 06:00 [medline]
AID - 10.14715/cmb/2022.68.9.24 [doi]
PST - epublish
SO  - Cell Mol Biol (Noisy-le-grand). 2022 Sep 30;68(9):150-159. doi: 
      10.14715/cmb/2022.68.9.24.