PMID- 36905457
OWN - NLM
STAT- MEDLINE
DCOM- 20230714
LR  - 20230718
IS  - 1877-8755 (Electronic)
IS  - 1138-7548 (Linking)
VI  - 79
IP  - 3
DP  - 2023 Aug
TI  - Guf1 overexpression improves pancreatic beta cell functions in type 2 diabetes 
      mellitus rats with Roux-en-Y gastric bypass (RYGB) surgery.
PG  - 569-582
LID - 10.1007/s13105-023-00952-6 [doi]
AB  - The Roux-en-Y gastric bypass (RYGB) is a one-of-a-kind treatment among 
      contemporary bariatric surgical procedures, and its therapeutic effects for type 
      2 diabetes mellitus (T2DM) are satisfactory. The present study performed isobaric 
      tags for relative and absolute quantification (iTRAQ) combined with liquid 
      chromatography-tandem mass spectrometry (LC-MS/MS) analysis identifying different 
      proteomics between T2DM rats with or without Roux-en-Y gastric bypass (RYGB) 
      surgery, and GTP binding elongation factor GUF1 (Guf1) was first found to be 
      significantly upregulated in rats from the T2DM plus RYGB group. In the cellular 
      lipotoxicity model induced by palmitic acid stimulation of rat pancreatic beta 
      cell line, INS-1, palmitic acid treatment inhibited cell viability, suppressed 
      GSIS, promoted lipid droplet formation, promoted cell apoptosis, and induced 
      mitochondrial membrane potential loss. The effects of palmitic acid on INS-1 
      cells mentioned above could be partially eliminated by Guf1 overexpression but 
      aggravated by Guf1 knockdown. Last, under palmitic acid treatment, Guf1 
      overexpression promotes the PI3K/Akt and NF-kappaB signaling but inhibits the AMPK 
      activation. Guf1 is upregulated in T2DM rats who received RYGB, and Guf1 
      overexpression improves cell mitochondrial functions, increases cell 
      proliferation, inhibits cell apoptosis, and promotes cell functions in palmitic 
      acid-treated beta cells.
CI  - (c) 2023. The Author(s) under exclusive licence to University of Navarra.
FAU - Li, Pengzhou
AU  - Li P
AD  - Department of General Surgery, Third Xiangya Hospital, Central South University, 
      Changsha, 410013, China.
FAU - Dai, Song
AU  - Dai S
AD  - Department of General Surgery, Third Xiangya Hospital, Central South University, 
      Changsha, 410013, China.
FAU - Gao, Xiang
AU  - Gao X
AD  - Department of General Surgery, Third Xiangya Hospital, Central South University, 
      Changsha, 410013, China.
FAU - Li, Weizheng
AU  - Li W
AUID- ORCID: 0000-0002-7183-9554
AD  - Department of General Surgery, Third Xiangya Hospital, Central South University, 
      Changsha, 410013, China. 2204060326@csu.edu.cn.
LA  - eng
GR  - YX202106/Wisdom Accumulation and Talent Cultivation Project of the Third Xiangya 
      Hospital of Central South University/
PT  - Journal Article
DEP - 20230311
PL  - Spain
TA  - J Physiol Biochem
JT  - Journal of physiology and biochemistry
JID - 9812509
RN  - 2V16EO95H1 (Palmitic Acid)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Diabetes Mellitus, Type 2/surgery/metabolism
MH  - *Gastric Bypass/methods
MH  - *Insulin-Secreting Cells/metabolism
MH  - Chromatography, Liquid
MH  - Palmitic Acid
MH  - Phosphatidylinositol 3-Kinases
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Guf1
OT  - Pancreatic beta cell
OT  - Roux-en-Y gastric bypass (RYGB)
OT  - Type 2 diabetes mellitus (T2DM)
EDAT- 2023/03/12 06:00
MHDA- 2023/07/14 13:07
CRDT- 2023/03/11 11:15
PHST- 2022/06/09 00:00 [received]
PHST- 2023/02/20 00:00 [accepted]
PHST- 2023/07/14 13:07 [medline]
PHST- 2023/03/12 06:00 [pubmed]
PHST- 2023/03/11 11:15 [entrez]
AID - 10.1007/s13105-023-00952-6 [pii]
AID - 10.1007/s13105-023-00952-6 [doi]
PST - ppublish
SO  - J Physiol Biochem. 2023 Aug;79(3):569-582. doi: 10.1007/s13105-023-00952-6. Epub 
      2023 Mar 11.