PMID- 36905507
OWN - NLM
STAT- MEDLINE
DCOM- 20230314
LR  - 20230424
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2618
DP  - 2023
TI  - In Vivo Tracking of Dendritic Cell Migration.
PG  - 39-53
LID - 10.1007/978-1-0716-2938-3_3 [doi]
AB  - Dendritic cells (DCs) in peripheral tissue serve as a sentinel to invasion and 
      maintain tolerance. They ingest and carry antigens to the draining lymph nodes 
      and present antigens to antigen-specific T cells to initiate acquired immune 
      responses. Thus, understanding DC migration from peripheral tissues and function 
      is critical for understanding DCs' roles in immune homeostasis. Here, we 
      introduced the KikGR in vivo photolabeling system, an ideal tool for monitoring 
      precise cellular movements and related functions in vivo under physiological 
      conditions and during various immune responses that occur in pathologic 
      condition. Using a mouse line expressing photoconvertible fluorescent protein 
      KikGR, we can label DCs in peripheral tissues by changing the color of KikGR from 
      green to red after exposure to violet light and accurately track DC migration 
      from each peripheral tissue to its respective draining lymph nodes.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Tomura, Michio
AU  - Tomura M
AD  - Laboratory of Immunology, Faculty of Pharmacy, Osaka Ohtani University, 
      Tondabayashi, Osaka, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Proteins)
RN  - 0 (Antigens)
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Dendritic Cells
MH  - Cell Movement
MH  - *Proteins
MH  - Antigens
MH  - Adaptive Immunity
MH  - Lymph Nodes
MH  - Mice, Inbred C57BL
OTO - NOTNLM
OT  - Dendritic cells
OT  - In vivo photo labeling
OT  - KikGR mouse
OT  - Migration
OT  - Photoconvertible protein
OT  - Tracking
EDAT- 2023/03/12 06:00
MHDA- 2023/03/15 06:00
CRDT- 2023/03/11 11:17
PHST- 2023/03/11 11:17 [entrez]
PHST- 2023/03/12 06:00 [pubmed]
PHST- 2023/03/15 06:00 [medline]
AID - 10.1007/978-1-0716-2938-3_3 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2618:39-53. doi: 10.1007/978-1-0716-2938-3_3.