PMID- 36905510
OWN - NLM
STAT- MEDLINE
DCOM- 20230322
LR  - 20230424
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2618
DP  - 2023
TI  - In Vitro Generation of Murine Bone Marrow-Derived Dendritic Cells.
PG  - 83-92
LID - 10.1007/978-1-0716-2938-3_6 [doi]
AB  - Dendritic cells (DCs) are mononuclear phagocytes of hematopoietic origin residing 
      in lymphoid and nonlymphoid tissues. DCs are often referred as the sentinels of 
      the immune system as they can sense pathogens and danger signals. Upon 
      activation, DCs migrate to the draining lymph nodes and present antigens to naive 
      T cells to trigger adaptive immunity. Hematopoietic progenitors for DCs reside in 
      the adult bone marrow (BM). Therefore, BM cell culture systems have been 
      developed to generate large amounts of primary DCs in vitro conveniently enabling 
      to analyze their developmental and functional features. Here, we review various 
      protocols enabling to generate DCs in vitro from murine BM cells and discuss the 
      cellular heterogeneity of each culture system.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Gerber-Ferder, Yohan
AU  - Gerber-Ferder Y
AD  - PSL University, Institut Curie Research Center, INSERM U932, Center for Cancers 
      Immunotherapy, Paris, France.
FAU - Bourdely, Pierre
AU  - Bourdely P
AD  - Universite de Paris, Centre for Inflammation Research, CNRS ERL8252, INSERM1149, 
      Paris, France.
FAU - Vetillard, Mathias
AU  - Vetillard M
AD  - Universite de Paris, Centre for Inflammation Research, CNRS ERL8252, INSERM1149, 
      Paris, France.
FAU - Guermonprez, Pierre
AU  - Guermonprez P
AD  - Universite de Paris, Centre for Inflammation Research, CNRS ERL8252, INSERM1149, 
      Paris, France.
FAU - Helft, Julie
AU  - Helft J
AD  - PSL University, Institut Curie Research Center, INSERM U932, Center for Cancers 
      Immunotherapy, Paris, France. julie.helft@inserm.fr.
AD  - Universite de Paris, Centre for Inflammation Research, CNRS ERL8252, INSERM1149, 
      Paris, France. julie.helft@inserm.fr.
AD  - Universite de Paris - Inserm - Cnrs, Institut Cochin, Paris, France. 
      julie.helft@inserm.fr.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Mice
MH  - *Bone Marrow
MH  - Bone Marrow Cells
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Dendritic Cells
MH  - Mice, Inbred C57BL
MH  - *T-Lymphocytes
OTO - NOTNLM
OT  - Bone marrow hematopoietic progenitors
OT  - Dendritic cells
OT  - FLT3L
OT  - GM-CSF
EDAT- 2023/03/12 06:00
MHDA- 2023/03/15 06:00
CRDT- 2023/03/11 11:17
PHST- 2023/03/11 11:17 [entrez]
PHST- 2023/03/12 06:00 [pubmed]
PHST- 2023/03/15 06:00 [medline]
AID - 10.1007/978-1-0716-2938-3_6 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2618:83-92. doi: 10.1007/978-1-0716-2938-3_6.