PMID- 36905512
OWN - NLM
STAT- MEDLINE
DCOM- 20230314
LR  - 20230424
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2618
DP  - 2023
TI  - In Vitro Generation of Murine CD8alpha(+) DEC205(+) XCR1(+) Cross-Presenting 
      Dendritic Cells from Bone Marrow-Derived Hematopoietic Progenitors.
PG  - 109-119
LID - 10.1007/978-1-0716-2938-3_8 [doi]
AB  - Dendritic cells (DCs) comprise a heterogeneous population of antigen 
      (Ag)-presenting cells that play a critical role in both innate and adaptive 
      immunity. DCs orchestrate protective responses against pathogens and tumors while 
      mediating tolerance to host tissues. Evolutionary conservation between species 
      has allowed the successful use of murine models to identify and characterize DC 
      types and functions relevant to human health. Among DCs, type 1 classical DCs 
      (cDC1) are uniquely capable of inducing antitumor responses and therefore present 
      a promising therapeutic target. However, the rarity of DCs, particularly cDC1, 
      limits the number of cells that can be isolated for study. Despite significant 
      effort, progress in the field has been hampered by inadequate methods to produce 
      large quantities of functionally mature DCs in vitro. To overcome this challenge, 
      we developed a culture system in which mouse primary bone marrow cells are 
      cocultured with OP9 stromal cells expressing Notch ligand Delta-like 1 (OP9-DL1) 
      to produce CD8alpha(+) DEC205(+) XCR1(+) cDC1 (Notch cDC1). This novel method 
      provides a valuable tool to facilitate the generation of unlimited cDC1 for 
      functional studies and translational applications such as antitumor vaccination 
      and immunotherapy.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Kirkling, Margaret E
AU  - Kirkling ME
AD  - Genomic Sciences- R&D, GSK, Collegeville, PA, USA.
FAU - Reizis, Boris
AU  - Reizis B
AD  - Department of Pathology, New York University School of Medicine, New York, NY, 
      USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Mice
MH  - Humans
MH  - Animals
MH  - *Bone Marrow
MH  - *Dendritic Cells
MH  - Bone Marrow Cells
MH  - Immunity
MH  - Coculture Techniques
OTO - NOTNLM
OT  - Adaptive immunity
OT  - Antigen cross-presentation
OT  - Antigen presentation
OT  - Antitumor
OT  - BMDC
OT  - CD8alpha+ dendritic cells
OT  - Classical dendritic cells
OT  - Immunotherapy
OT  - Innate immunity
OT  - Notch signaling
OT  - OP9-DL1
OT  - cDC1
EDAT- 2023/03/12 06:00
MHDA- 2023/03/15 06:00
CRDT- 2023/03/11 11:17
PHST- 2023/03/11 11:17 [entrez]
PHST- 2023/03/12 06:00 [pubmed]
PHST- 2023/03/15 06:00 [medline]
AID - 10.1007/978-1-0716-2938-3_8 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2618:109-119. doi: 10.1007/978-1-0716-2938-3_8.