PMID- 36905513
OWN - NLM
STAT- MEDLINE
DCOM- 20230314
LR  - 20230424
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2618
DP  - 2023
TI  - In Vitro Generation of Human Dendritic Cell Subsets from CD34+ Cord Blood 
      Progenitors.
PG  - 121-132
LID - 10.1007/978-1-0716-2938-3_9 [doi]
AB  - Dendritic cells (DCs) are professional antigen-presenting cells controlling the 
      activation of T cells and thus regulating adaptive immune response against 
      pathogens or tumors. Modeling human DC differentiation and function is crucial 
      for our understanding of immune response and the development of new therapies. 
      Considering DC rarity in human blood, in vitro systems allowing their faithful 
      generation are needed. This chapter will describe a DC differentiation method 
      based on the co-culture of CD34(+) cord blood progenitors together with 
      mesenchymal stromal cells (eMSCs) engineered to deliver growth factors and 
      chemokines.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Bourdely, Pierre
AU  - Bourdely P
AD  - Institut Cochin, INSERM U1016, CNRS UMR8104, Universite de Paris Cite, Paris, 
      France. pierre.bourdely@inserm.fr.
FAU - Savoldelli, Roberto
AU  - Savoldelli R
AD  - King's College London, Centre for Inflammation Biology and Cancer Immunology, 
      London, UK.
AD  - Universite de Paris, INSERM U1149, CNRS erl8252, Centre for Inflammation 
      Research, Universite de Paris Cite, Paris, France.
FAU - Vetillard, Mathias
AU  - Vetillard M
AD  - Universite de Paris, INSERM U1149, CNRS erl8252, Centre for Inflammation 
      Research, Universite de Paris Cite, Paris, France.
FAU - Anselmi, Giorgio
AU  - Anselmi G
AD  - King's College London, Centre for Inflammation Biology and Cancer Immunology, 
      London, UK.
AD  - Oxford University, Radcliffe Department of Medicine, Oxford, UK.
FAU - Helft, Julie
AU  - Helft J
AD  - Institut Cochin, INSERM U1016, CNRS UMR8104, Universite de Paris Cite, Paris, 
      France.
FAU - Guermonprez, Pierre
AU  - Guermonprez P
AD  - Universite de Paris, INSERM U1149, CNRS erl8252, Centre for Inflammation 
      Research, Universite de Paris Cite, Paris, France. pierre.guermonprez@cnrs.fr.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Antigens, CD34)
RN  - 0 (Cell Adhesion Molecules)
SB  - IM
MH  - Humans
MH  - *Dendritic Cells
MH  - *Fetal Blood
MH  - Cells, Cultured
MH  - Antigens, CD34/metabolism
MH  - Cell Differentiation
MH  - Cell Adhesion Molecules
OTO - NOTNLM
OT  - CD34
OT  - Conventional dendritic cells
OT  - Cord blood
OT  - Dendritic cells
OT  - Hematopoietic stem cells
OT  - In vitro differentiation
OT  - Mesenchymal stromal cells
OT  - Plasmacytoid dendritic cell
EDAT- 2023/03/12 06:00
MHDA- 2023/03/15 06:00
CRDT- 2023/03/11 11:17
PHST- 2023/03/11 11:17 [entrez]
PHST- 2023/03/12 06:00 [pubmed]
PHST- 2023/03/15 06:00 [medline]
AID - 10.1007/978-1-0716-2938-3_9 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2618:121-132. doi: 10.1007/978-1-0716-2938-3_9.