PMID- 36905516
OWN - NLM
STAT- MEDLINE
DCOM- 20230314
LR  - 20230424
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2618
DP  - 2023
TI  - Clonal Analysis of Human Dendritic Cell Progenitors Using a Stromal Cell Culture.
PG  - 155-170
LID - 10.1007/978-1-0716-2938-3_12 [doi]
AB  - Dendritic cells (DCs) are a heterogenous population of professional 
      antigen-presenting cells that play an "educator" role in immunity. Multiple DC 
      subsets collaboratively initiate and orchestrate innate and adaptive immune 
      responses. Recent advances in our ability to investigate cellular transcription, 
      signaling, and function at the single-cell level have opened opportunities to 
      examine heterogeneous populations at unprecedented resolutions. Culturing of 
      mouse DC subsets from single bone marrow hematopoietic progenitor cells, that is, 
      clonal analysis, has enabled identification of multiple progenitors with distinct 
      potentials and furthered understanding of mouse DC development. However, studies 
      of human DC development have been hampered by the lack of a corresponding system 
      to generate multiple human DC subsets. Here, we describe a protocol to 
      functionally profile the differentiation potentials of single human hematopoietic 
      stem and progenitor cells (HSPCs) to multiple DC subsets, myeloid and lymphoid 
      cells that will facilitate investigation of human DC lineage specification and 
      reveal its molecular bases.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Liu, Kang
AU  - Liu K
AD  - Cancer Immunology and Immune Modulation, Boehringer Ingelheim, Ridgefield, CT, 
      USA. kang_3.liu@boehringer-ingelheim.com.
FAU - Lee, Jaeyop
AU  - Lee J
AD  - Memorial Sloan Kettering Cancer Center, New York, NY, USA.
FAU - Luh, Thomas
AU  - Luh T
AD  - University of California San Francisco, School of Medicine, San Francisco, CA, 
      USA.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Humans
MH  - Mice
MH  - Animals
MH  - *Dendritic Cells
MH  - *Hematopoietic Stem Cells
MH  - Cell Differentiation
MH  - Stromal Cells
MH  - Cell Culture Techniques
OTO - NOTNLM
OT  - CD34+
OT  - Cord blood
OT  - Culture
OT  - Dendritic cell
OT  - Differentiation
OT  - Flow cytometry
OT  - Human
OT  - Progenitor
OT  - Single cell
OT  - Stromal cells
EDAT- 2023/03/12 06:00
MHDA- 2023/03/15 06:00
CRDT- 2023/03/11 11:17
PHST- 2023/03/11 11:17 [entrez]
PHST- 2023/03/12 06:00 [pubmed]
PHST- 2023/03/15 06:00 [medline]
AID - 10.1007/978-1-0716-2938-3_12 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2618:155-170. doi: 10.1007/978-1-0716-2938-3_12.