PMID- 36905517
OWN - NLM
STAT- MEDLINE
DCOM- 20230314
LR  - 20230424
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2618
DP  - 2023
TI  - Enrichment of Large Numbers of Splenic Mouse Dendritic Cells After Injection of 
      Flt3L-Producing Tumor Cells.
PG  - 173-186
LID - 10.1007/978-1-0716-2938-3_13 [doi]
AB  - Dendritic cells (DCs) are antigen-presenting cells (APCs) that shape innate and 
      adaptive immunity. There are multiple subsets of DCs distinguished according to 
      their phenotype and functional specialization. DCs are present in lymphoid organs 
      and across multiple tissues. However, their frequency and numbers at these sites 
      are very low making their functional study difficult. Multiple protocols have 
      been developed to generate DCs in vitro from bone marrow progenitors, but they do 
      not fully recapitulate DC complexity found in vivo. Therefore, directly 
      amplifying endogenous DCs in vivo appears as an option to overcome this specific 
      caveat. In this chapter, we describe a protocol to amplify murine DCs in vivo by 
      the injection of a B16 melanoma cell line expressing the trophic factor FMS-like 
      tyrosine kinase 3 ligand (Flt3L). We have also compared two methods of magnetic 
      sorting of amplified DCs, both giving high yields of total murine DCs, but 
      different representation of the main DC subsets found in vivo.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Santa, Pauline
AU  - Santa P
AD  - Universite de Bordeaux, CNRS, ImmunoConcEpT, UMR 5164, Bordeaux, France.
FAU - Roubertie, Anais
AU  - Roubertie A
AD  - Universite de Bordeaux, CNRS, ImmunoConcEpT, UMR 5164, Bordeaux, France.
FAU - Loizon, Severine
AU  - Loizon S
AD  - Universite de Bordeaux, CNRS, ImmunoConcEpT, UMR 5164, Bordeaux, France.
FAU - Garreau, Anne
AU  - Garreau A
AD  - Universite de Bordeaux, CNRS, ImmunoConcEpT, UMR 5164, Bordeaux, France.
FAU - Ferriere, Amandine
AU  - Ferriere A
AD  - Universite de Bordeaux, CNRS, ImmunoConcEpT, UMR 5164, Bordeaux, France.
FAU - Duluc, Dorothee
AU  - Duluc D
AD  - Universite de Bordeaux, CNRS, ImmunoConcEpT, UMR 5164, Bordeaux, France. 
      dorothee.duluc@u-bordeaux.fr.
FAU - Sisirak, Vanja
AU  - Sisirak V
AD  - Universite de Bordeaux, CNRS, ImmunoConcEpT, UMR 5164, Bordeaux, France. 
      vanja.sisirak@u-bordeaux.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (flt3 ligand protein)
RN  - 0 (Membrane Proteins)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Membrane Proteins/metabolism
MH  - *Dendritic Cells
MH  - Spleen/metabolism
OTO - NOTNLM
OT  - Conventional dendritic cells
OT  - DC enrichment
OT  - Flt3L
OT  - Plasmacytoid dendritic cells
EDAT- 2023/03/12 06:00
MHDA- 2023/03/15 06:00
CRDT- 2023/03/11 11:17
PHST- 2023/03/11 11:17 [entrez]
PHST- 2023/03/12 06:00 [pubmed]
PHST- 2023/03/15 06:00 [medline]
AID - 10.1007/978-1-0716-2938-3_13 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2618:173-186. doi: 10.1007/978-1-0716-2938-3_13.