PMID- 36905518 OWN - NLM STAT- MEDLINE DCOM- 20230314 LR - 20230424 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 2618 DP - 2023 TI - Isolation and Identification of Dendritic Cell Subsets from Human and Mouse Tumors. PG - 187-197 LID - 10.1007/978-1-0716-2938-3_14 [doi] AB - Dendritic cells (DCs) are professional antigen-presenting cells (APCs) that have the ability to orchestrate adaptive and innate immune responses by antigen phagocytosis and T cell activation across different inflammatory settings such as tumor development. As specific DC identity and how these cells interact with their neighbors is still not fully understood, it remains a challenge to unravel DC heterogeneity, particularly in human cancers. In this chapter, we describe a protocol to isolate and characterize tumor-infiltrating DCs. CI - (c) 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Rocca, Yamila AU - Rocca Y AD - Centre de Recherche en Cancerologie de Lyon, INSERM U1052, CNRS UMR5286, Universite de Lyon, Universite Lyon 1, Centre Leon Berard, Lyon, France. FAU - Voissiere, Aurelien AU - Voissiere A AD - Centre de Recherche en Cancerologie de Lyon, INSERM U1052, CNRS UMR5286, Universite de Lyon, Universite Lyon 1, Centre Leon Berard, Lyon, France. FAU - Valladeau-Guilemond, Jenny AU - Valladeau-Guilemond J AD - Centre de Recherche en Cancerologie de Lyon, INSERM U1052, CNRS UMR5286, Universite de Lyon, Universite Lyon 1, Centre Leon Berard, Lyon, France. FAU - Bendriss-Vermare, Nathalie AU - Bendriss-Vermare N AD - Centre de Recherche en Cancerologie de Lyon, INSERM U1052, CNRS UMR5286, Universite de Lyon, Universite Lyon 1, Centre Leon Berard, Lyon, France. nathalie.bendriss-vermare@lyon.unicancer.fr. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 SB - IM MH - Humans MH - Mice MH - Animals MH - *Dendritic Cells MH - Lymphocyte Activation MH - *Neoplasms/pathology MH - Phagocytosis OTO - NOTNLM OT - DC identification OT - DC isolation OT - DC subsets OT - Dendritic cells OT - Human OT - Mouse OT - Tumors EDAT- 2023/03/12 06:00 MHDA- 2023/03/15 06:00 CRDT- 2023/03/11 11:17 PHST- 2023/03/11 11:17 [entrez] PHST- 2023/03/12 06:00 [pubmed] PHST- 2023/03/15 06:00 [medline] AID - 10.1007/978-1-0716-2938-3_14 [doi] PST - ppublish SO - Methods Mol Biol. 2023;2618:187-197. doi: 10.1007/978-1-0716-2938-3_14.