PMID- 36905525
OWN - NLM
STAT- MEDLINE
DCOM- 20230314
LR  - 20230424
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2618
DP  - 2023
TI  - Tracking Plasmacytoid Dendritic Cell Response to Physical Contact with Infected 
      Cells.
PG  - 289-315
LID - 10.1007/978-1-0716-2938-3_21 [doi]
AB  - Dendritic cells (DCs) are key regulators of both innate and adaptive immunity via 
      varied functions, including cytokine production and antigen presentation. 
      Plasmacytoid DC (pDC) is a DC subset specialized in the production of type I and 
      III interferons (IFNs). They are thus pivotal players of the host antiviral 
      response during the acute phase of infection by genetically distant viruses. The 
      pDC response is primarily triggered by the endolysosomal sensors Toll-like 
      receptors, which recognize nucleic acids from pathogens. In some pathologic 
      contexts, pDC response can also be triggered by host nucleic acids, hereby 
      contributing to the pathogenesis of autoimmune diseases, such as, e.g., systemic 
      lupus erythematosus. Importantly, recent in vitro studies from our laboratory and 
      others uncovered that pDCs sense viral infections when a physical contact is 
      established with infected cells. This specialized synapse-like feature enables a 
      robust type I and III IFN secretion at the infected site. Therefore, this 
      concentrated and confined response likely limits the correlated deleterious 
      impacts of excessive cytokine production to the host, notably due to tissue 
      damages. Here we provide a pipeline of methods for ex vivo studies of pDC 
      antiviral functions, designed to address how pDC activation is regulated by 
      cell-cell contact with virally infected cells and the current approaches enabling 
      to decipher the underlying molecular events leading to an efficient antiviral 
      response.
CI  - (c) 2023. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Ribeiro, Margarida Sa
AU  - Ribeiro MS
AD  - CIRI, Inserm, U1111, Universite Claude Bernard Lyon 1, CNRS, UMR5308, Ecole 
      Normale Superieure de Lyon, Univ Lyon, Lyon, France.
FAU - Joshi, Garima
AU  - Joshi G
AD  - CIRI, Inserm, U1111, Universite Claude Bernard Lyon 1, CNRS, UMR5308, Ecole 
      Normale Superieure de Lyon, Univ Lyon, Lyon, France.
FAU - Decembre, Elodie
AU  - Decembre E
AD  - CIRI, Inserm, U1111, Universite Claude Bernard Lyon 1, CNRS, UMR5308, Ecole 
      Normale Superieure de Lyon, Univ Lyon, Lyon, France.
FAU - Nuovo, Celia
AU  - Nuovo C
AD  - CIRI, Inserm, U1111, Universite Claude Bernard Lyon 1, CNRS, UMR5308, Ecole 
      Normale Superieure de Lyon, Univ Lyon, Lyon, France.
FAU - Bosseboeuf, Adrien
AU  - Bosseboeuf A
AD  - CIRI, Inserm, U1111, Universite Claude Bernard Lyon 1, CNRS, UMR5308, Ecole 
      Normale Superieure de Lyon, Univ Lyon, Lyon, France.
FAU - Bellomo, Alicia
AU  - Bellomo A
AD  - CIRI, Inserm, U1111, Universite Claude Bernard Lyon 1, CNRS, UMR5308, Ecole 
      Normale Superieure de Lyon, Univ Lyon, Lyon, France.
FAU - Venet, Manon
AU  - Venet M
AD  - CIRI, Inserm, U1111, Universite Claude Bernard Lyon 1, CNRS, UMR5308, Ecole 
      Normale Superieure de Lyon, Univ Lyon, Lyon, France.
FAU - Assil, Sonia
AU  - Assil S
AD  - CIRI, Inserm, U1111, Universite Claude Bernard Lyon 1, CNRS, UMR5308, Ecole 
      Normale Superieure de Lyon, Univ Lyon, Lyon, France.
FAU - Dreux, Marlene
AU  - Dreux M
AD  - CIRI, Inserm, U1111, Universite Claude Bernard Lyon 1, CNRS, UMR5308, Ecole 
      Normale Superieure de Lyon, Univ Lyon, Lyon, France. marlene.dreux@ens-lyon.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Antiviral Agents)
RN  - 9008-11-1 (Interferons)
RN  - 0 (Nucleic Acids)
RN  - 0 (Interferon Type I)
SB  - IM
MH  - Immunity, Innate
MH  - Antiviral Agents
MH  - Interferons
MH  - Dendritic Cells
MH  - *Nucleic Acids
MH  - *Interferon Type I/metabolism
OTO - NOTNLM
OT  - Cell-cell contact
OT  - Confocal microscopy analysis
OT  - Imaging flow cytometry
OT  - Inflammation
OT  - Innate immunity
OT  - Interferon
OT  - Live imaging
OT  - Plasmacytoid dendritic cells
OT  - Toll-like receptor
OT  - Virus
EDAT- 2023/03/12 06:00
MHDA- 2023/03/15 06:00
CRDT- 2023/03/11 11:17
PHST- 2023/03/11 11:17 [entrez]
PHST- 2023/03/12 06:00 [pubmed]
PHST- 2023/03/15 06:00 [medline]
AID - 10.1007/978-1-0716-2938-3_21 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2023;2618:289-315. doi: 10.1007/978-1-0716-2938-3_21.