PMID- 36905790
OWN - NLM
STAT- MEDLINE
DCOM- 20230405
LR  - 20230405
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 258
DP  - 2023 Jun 1
TI  - Open flow cytometer with the combination of 3D hydrodynamic single cell focusing 
      and confocal laser-induced fluorescence detection.
PG  - 124424
LID - S0039-9140(23)00175-3 [pii]
LID - 10.1016/j.talanta.2023.124424 [doi]
AB  - Flow cytometry is among the most powerful tools for single-cell analysis, while 
      the high cost and mechanical complexity of the commercial instrumentation limit 
      the applications in personalized single-cell analysis. For this issue, we hereby 
      construct an open and low-cost flow cytometer. It is highly compact to integrate 
      the functions of (1) single cell aligning by a lab-made modularized 3D 
      hydrodynamic focusing device, and (2) fluorescence detection of the single cells 
      by a confocal laser-induced fluorescence (LIF) detector. The ceiling cost of the 
      entire hardware for the LIF detection unit and 3D focusing device is $ 3200 and $ 
      400 respectively. A sheath flow velocity of 150 muL/min produces a focused sample 
      stream of 17.6 mum x 14.6 mum at sample flow of 2 muL/min, based on the LIF response 
      frequency and the laser beam spot diameter. The assay performance of the flow 
      cytometer was evaluated by characterizing fluorescent microparticles and acridine 
      orange (AO) stained HepG2 cells, producing throughputs of 40.5/s and 6.2/s 
      respectively. Favorable assay precision and accuracy were demonstrated by the 
      agreement of frequency histogram with imaging analysis, and good Gaussian-like 
      distributions of fluorescent microparticles and AO-stained HepG2 cells. 
      Practically, the flow cytometer was successfully applied for the evaluation of 
      ROS generation in single HepG2 cells.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Wu, Chengxin
AU  - Wu C
AD  - Research Center for Analytical Sciences, Department of Chemistry, College of 
      Sciences, Northeastern University, Shenyang, 110819, China.
FAU - Wei, Xing
AU  - Wei X
AD  - Research Center for Analytical Sciences, Department of Chemistry, College of 
      Sciences, Northeastern University, Shenyang, 110819, China.
FAU - Men, Xue
AU  - Men X
AD  - Research Center for Analytical Sciences, Department of Chemistry, College of 
      Sciences, Northeastern University, Shenyang, 110819, China.
FAU - Xu, Yulong
AU  - Xu Y
AD  - Research Center for Analytical Sciences, Department of Chemistry, College of 
      Sciences, Northeastern University, Shenyang, 110819, China.
FAU - Bai, Junjie
AU  - Bai J
AD  - Research Center for Analytical Sciences, Department of Chemistry, College of 
      Sciences, Northeastern University, Shenyang, 110819, China.
FAU - Wang, Yu
AU  - Wang Y
AD  - Research Center for Analytical Sciences, Department of Chemistry, College of 
      Sciences, Northeastern University, Shenyang, 110819, China.
FAU - Zhou, Lei
AU  - Zhou L
AD  - State Key Laboratory of Applied Organic Chemistry, College of Chemistry and 
      Chemical Engineering, Lanzhou University, Lanzhou, 730000, China.
FAU - Yu, Yong-Liang
AU  - Yu YL
AD  - Research Center for Analytical Sciences, Department of Chemistry, College of 
      Sciences, Northeastern University, Shenyang, 110819, China.
FAU - Xu, Zhang-Run
AU  - Xu ZR
AD  - Research Center for Analytical Sciences, Department of Chemistry, College of 
      Sciences, Northeastern University, Shenyang, 110819, China.
FAU - Chen, Ming-Li
AU  - Chen ML
AD  - Research Center for Analytical Sciences, Department of Chemistry, College of 
      Sciences, Northeastern University, Shenyang, 110819, China. Electronic address: 
      chenml@mail.neu.edu.cn.
FAU - Wang, Jian-Hua
AU  - Wang JH
AD  - Research Center for Analytical Sciences, Department of Chemistry, College of 
      Sciences, Northeastern University, Shenyang, 110819, China. Electronic address: 
      jianhuajrz@mail.neu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230306
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Coloring Agents)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Flow Cytometry/methods
MH  - *Hydrodynamics
MH  - *Coloring Agents
MH  - Acridine Orange
MH  - Lasers
OTO - NOTNLM
OT  - Flow cytometry
OT  - High-throughput
OT  - Laser-induced fluorescence
OT  - Sheath flow
OT  - Single-cell analysis
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/03/12 06:00
MHDA- 2023/04/05 06:42
CRDT- 2023/03/11 18:11
PHST- 2023/01/28 00:00 [received]
PHST- 2023/02/26 00:00 [revised]
PHST- 2023/03/04 00:00 [accepted]
PHST- 2023/04/05 06:42 [medline]
PHST- 2023/03/12 06:00 [pubmed]
PHST- 2023/03/11 18:11 [entrez]
AID - S0039-9140(23)00175-3 [pii]
AID - 10.1016/j.talanta.2023.124424 [doi]
PST - ppublish
SO  - Talanta. 2023 Jun 1;258:124424. doi: 10.1016/j.talanta.2023.124424. Epub 2023 Mar 
      6.