PMID- 36906097
OWN - NLM
STAT- MEDLINE
DCOM- 20230404
LR  - 20230425
IS  - 1096-0384 (Electronic)
IS  - 0003-9861 (Linking)
VI  - 739
DP  - 2023 May 1
TI  - Estimation of energy pathway fluxes in cancer cells - Beyond the Warburg effect.
PG  - 109559
LID - S0003-9861(23)00058-9 [pii]
LID - 10.1016/j.abb.2023.109559 [doi]
AB  - Glycolytic and respiratory fluxes were analyzed in cancer and non-cancer cells. 
      The steady-state fluxes in energy metabolism were used to estimate the 
      contributions of aerobic glycolytic and oxidative phosphorylation (OxPhos) 
      pathways to the cellular ATP supply. The rate of lactate production - corrected 
      for the fraction generated by glutaminolysis - is proposed as the appropriate way 
      to estimate glycolytic flux. In general, the glycolytic rates estimated for 
      cancer cells are higher than those found in non-cancer cells, as originally 
      observed by Otto Warburg. The rate of basal or endogenous cellular O(2) 
      consumption corrected for non-ATP synthesizing O(2) consumption, measured after 
      inhibition by oligomycin (a specific, potent and permeable ATP synthase 
      inhibitor), has been proposed as the appropriate way to estimate mitochondrial 
      ATP synthesis-linked O(2) flux or net OxPhos flux in living cells. Detecting 
      non-negligible oligomycin-sensitive O(2) consumption rates in cancer cells has 
      revealed that the mitochondrial function is not impaired, as claimed by the 
      Warburg effect. Furthermore, when calculating the relative contributions to 
      cellular ATP supply, under a variety of environmental conditions and for 
      different types of cancer cells, it was found that OxPhos pathway was the main 
      ATP provider over glycolysis. Hence, OxPhos pathway targeting can be successfully 
      used to block in cancer cells ATP-dependent processes such as migration. These 
      observations may guide the re-design of novel targeted therapies.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Moreno-Sanchez, Rafael
AU  - Moreno-Sanchez R
AD  - Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de 
      Mexico, Carrera de Biologia, Laboratorio de Control Metabolico, Los Reyes 
      Ixtacala, Hab. Los Reyes Ixtacala Barrio de los Arboles/Barrio de los Heroes, 
      Tlalnepantla, 54090, Mexico. Electronic address: rafael.moreno@iztacala.unam.mx.
FAU - Robledo-Cadena, Diana Xochiquetzal
AU  - Robledo-Cadena DX
AD  - Instituto Nacional de Cardiologia, Departamento de Bioquimica, Ciudad de Mexico, 
      14080, Mexico.
FAU - Pacheco-Velazquez, Silvia Cecilia
AU  - Pacheco-Velazquez SC
AD  - Instituto Nacional de Cardiologia, Departamento de Bioquimica, Ciudad de Mexico, 
      14080, Mexico.
FAU - Vargas Navarro, Jorge Luis
AU  - Vargas Navarro JL
AD  - Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de 
      Mexico, Carrera de Biologia, Laboratorio de Control Metabolico, Los Reyes 
      Ixtacala, Hab. Los Reyes Ixtacala Barrio de los Arboles/Barrio de los Heroes, 
      Tlalnepantla, 54090, Mexico.
FAU - Padilla-Flores, Joaquin Alberto
AU  - Padilla-Flores JA
AD  - Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de 
      Mexico, Carrera de Biologia, Laboratorio de Control Metabolico, Los Reyes 
      Ixtacala, Hab. Los Reyes Ixtacala Barrio de los Arboles/Barrio de los Heroes, 
      Tlalnepantla, 54090, Mexico.
FAU - Rodriguez-Enriquez, Sara
AU  - Rodriguez-Enriquez S
AD  - Instituto Nacional de Cardiologia, Departamento de Bioquimica, Ciudad de Mexico, 
      14080, Mexico; Facultad de Estudios Superiores Iztacala, Universidad Nacional 
      Autonoma de Mexico, Carrera de Medicina, Laboratorio de Control Metabolico, Los 
      Reyes Ixtacala, Hab. Los Reyes Ixtacala Barrio de los Arboles/Barrio de los 
      Heroes, Tlalnepantla, 54090, Mexico. Electronic address: saren960104@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230309
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - *Adenosine Triphosphate/metabolism
MH  - Energy Metabolism
MH  - Glycolysis/physiology
MH  - Oxidative Phosphorylation
MH  - Citric Acid Cycle
MH  - *Neoplasms
OTO - NOTNLM
OT  - ATP supply In cancer cells
OT  - Glycolysis
OT  - Metastasis
OT  - Oxidative phosphorylation
COIS- Declaration of competing interest The authors declare no conflicts of interest.
EDAT- 2023/03/12 06:00
MHDA- 2023/04/04 06:42
CRDT- 2023/03/11 19:28
PHST- 2022/10/24 00:00 [received]
PHST- 2023/02/15 00:00 [revised]
PHST- 2023/03/04 00:00 [accepted]
PHST- 2023/04/04 06:42 [medline]
PHST- 2023/03/12 06:00 [pubmed]
PHST- 2023/03/11 19:28 [entrez]
AID - S0003-9861(23)00058-9 [pii]
AID - 10.1016/j.abb.2023.109559 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 2023 May 1;739:109559. doi: 10.1016/j.abb.2023.109559. Epub 
      2023 Mar 9.